Aberrant Expression of Some Circulating miRNAs in Childhood Acute Lymphoblastic Leukemia

Swellam, Menha; Hashim, Maha; Mahmoud, Magda Sayed; Ramadan, Amal; Hassan, Noha

doi:10.1007/s10528-018-9844-y

Cited by 22 publications

(18 citation statements)

References 27 publications

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“…Swellam et al[ 46 ] investigated the expression signature of miRNA-125b-1 and miRNA-203 among childhood ALL, and they proposed these miRNAs as useful molecular markers for the diagnosis of childhood ALL. They noticed that the expression level of miRNA-125b-1 was significantly higher in peripheral blood (PB) isolated from 43 newly diagnosed children with ALL, while the miRNA-203 level was significantly lower in childhood ALL compared to control samples.…”

Section: Role Of Microrna In Diagnosis and Classificationmentioning

confidence: 99%

“…They noticed that the expression level of miRNA-125b-1 was significantly higher in peripheral blood (PB) isolated from 43 newly diagnosed children with ALL, while the miRNA-203 level was significantly lower in childhood ALL compared to control samples. Moreover, miRNA-125-1 was increased in T-ALL compared to other ALL phenotypes, and the miRNA-203 expression level was high in T-ALL followed by pre-B-ALL[ 46 ].…”

Section: Role Of Microrna In Diagnosis and Classificationmentioning

confidence: 99%

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Role of microRNA dysregulation in childhood acute leukemias: diagnostics, monitoring and therapeutics: A comprehensive review

Szczepanek

2020

WJCO

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MicroRNAs (miRNAs) are short noncoding RNAs that regulate the expression of genes by sequence-specific binding to mRNA to either promote or block its translation; they can also act as tumor suppressors ( e.g ., let-7b, miR-29a, miR-99, mir-100, miR-155, and miR-181) and/or oncogenes ( e.g ., miR-29a, miR-125b, miR-143-p3, mir-155, miR-181, miR-183, miR-196b, and miR-223) in childhood acute leukemia (AL). Differentially expressed miRNAs are important factors associated with the initiation and progression of AL. As shown in many studies, they can be used as noninvasive diagnostic and prognostic biomarkers, which are useful in monitoring early stages of AL development or during therapy ( e.g ., miR-125b, miR-146b, miR-181c, and miR-4786), accurate classification of different cellular or molecular AL subgroups ( e.g ., let-7b, miR-98, miR-100, miR-128b, and miR-223), and identification and development of new therapeutic agents ( e.g ., mir-10, miR-125b, miR-203, miR-210, miR-335). Specific miRNA patterns have also been described for commonly used AL therapy drugs ( e.g ., miR-125b and miR-223 for doxorubicin, miR-335 and miR-1208 for prednisolone, and miR-203 for imatinib), uncovering miRNAs that are associated with treatment response. In the current review, the role of miRNAs in the development, progression, and therapy monitoring of pediatric ALs will be presented and discussed.

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Section: Role Of Microrna In Diagnosis and Classificationmentioning

confidence: 99%

Section: Role Of Microrna In Diagnosis and Classificationmentioning

confidence: 99%

Role of microRNA dysregulation in childhood acute leukemias: diagnostics, monitoring and therapeutics: A comprehensive review

Szczepanek

2020

WJCO

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“…A study discovered the abnormal expression of miRNAs in blood samples of children with AL, and further indicated that miR-125b and miR-203 are potential molecular markers for diagnosing childhood AL (18). In addition, the study by Swellam et al (19) denoted that the levels of miR-143 and miR-182 in bone marrow samples of children with AL are decreased, and the prognosis of patients is poor. It reveals that abnormally expressed miRNAs play a key role in the development and progression of childhood AL.…”

Section: Discussionmentioning

confidence: 99%

GEM on proliferation and apoptosis of childhood AL cells through inhibiting c‑myc expression by upregulating miR‑125a‑3p

Xing

Yin

et al. 2020

Oncol Lett

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Effect of gemcitabine (GEM) on proliferation and apoptosis of childhood acute leukemia (AL) cells and the mechanism of action were investigated. Bone marrow and peripheral blood of 18 newly diagnosed children with childhood AL admitted to Yidu Central Hospital of Weifang were selected, and the miR-125a-3p level in peripheral blood of healthy children and children with AL was detected by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Leukemia cells from the bone marrow of children with AL were primarily cultured and purified to observe the morphology. miR-125a-3p mimic was transfected into childhood AL cells. The cells were randomly divided into three groups: control group, GEM group and GEM + miR-125a-3p mimic group. 5-ethynyl-2'-deoxyuridine (EdU) staining assay was chosen to detect the proliferation of childhood AL cells in each group. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining assay was adopted to determine apoptosis of childhood AL cells. The protein level of c-myc was measured via western blotting. Compared with that in the healthy children, the level of miR-125a-3p in the peripheral blood of children with AL was remarkably decreased. Compared with those in the control group, GEM inhibited proliferation and promoted apoptosis of childhood AL cells, and impeded the protein expression of c-myc in these cells. Compared with those in the GEM group, GEM + miR-125a-3p mimic notably reduced the proliferation and enhanced apoptosis of cells, and the protein expression of c-myc in cells was overtly reduced. The level of miR-125a-3p in peripheral blood of children with AL is obviously decreased. It is suggested in this study that GEM can inhibit the proliferation and promote apoptosis of childhood AL cells, and the mechanism may be related to upregulated miR-125a-3p inhibiting the expression of c-myc.

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“…Significant higher median levels of miR-100, miR-196a, and miR-146a were reported in blood samples of affected children compared to controls, but the diagnostic efficacy for miR-146a analysis presented superior sensitivity and specificity [ 211 ]. These same authors recently reported the significant increased expression of circulating miR-125b-1 and low levels of miR-203 in serum samples from untreated newly diagnosed children with ALL ( n = 43), as detected by quantitative RT-PCR analysis [ 212 ]. They also showed higher levels of miR-125b-1 in T-ALL samples as compared to other ALL phenotypes [ 212 ].…”

Section: Mirnas In Clinicsmentioning

confidence: 99%

“…These same authors recently reported the significant increased expression of circulating miR-125b-1 and low levels of miR-203 in serum samples from untreated newly diagnosed children with ALL ( n = 43), as detected by quantitative RT-PCR analysis [ 212 ]. They also showed higher levels of miR-125b-1 in T-ALL samples as compared to other ALL phenotypes [ 212 ]. More recently, circulating miR-652-3p was found downregulated in serum from ALL patients and levels reported as restored when patients attained in complete remission [ 47 ].…”

Section: Mirnas In Clinicsmentioning

confidence: 99%

MiRNA Dysregulation in Childhood Hematological Cancer

Oliveira

Roberto

Baroni

et al. 2018

IJMS

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For decades, cancer biology focused largely on the protein-encoding genes that have clear roles in tumor development or progression: cell-cycle control, apoptotic evasion, genome instability, drug resistance, or signaling pathways that stimulate growth, angiogenesis, or metastasis. MicroRNAs (miRNAs), however, represent one of the more abundant classes of cell modulators in multicellular organisms and largely contribute to regulating gene expression. Many of the ~2500 miRNAs discovered to date in humans regulate vital biological processes, and their aberrant expression results in pathological and malignant outcomes. In this review, we highlight what has been learned about the roles of miRNAs in some of the most common human pediatric leukemias and lymphomas, along with their value as diagnostic/prognostic factors.

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Aberrant Expression of Some Circulating miRNAs in Childhood Acute Lymphoblastic Leukemia

Cited by 22 publications

References 27 publications

Role of microRNA dysregulation in childhood acute leukemias: diagnostics, monitoring and therapeutics: A comprehensive review

Role of microRNA dysregulation in childhood acute leukemias: diagnostics, monitoring and therapeutics: A comprehensive review

GEM on proliferation and apoptosis of childhood AL cells through inhibiting c‑myc expression by upregulating miR‑125a‑3p

MiRNA Dysregulation in Childhood Hematological Cancer

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