Aberrant expression of vimentin predisposes oral premalignant lesion derived cells towards transformation

Dmello, Crismita; Sawant, Sharada; Chaudhari, Pratik R.; Dongre, Harsh; Ahire, Chetan; D'Souza, Zinia Charlotte; Charles, Silvania Emlit; Rane, Pallavi; Costea, Daniela Elena; Chaukar, Davendra; Kane, Shubhada; Vaidya, Milind M.

doi:10.1016/j.yexmp.2018.08.010

Cited by 11 publications

(15 citation statements)

References 22 publications

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“…Many studies found an abnormal expression of vimentin in a variety of epithelial tumors including OSCC and its aberrant expression is an independent adverse prognostic factor in OSCC as evaluated by multivariate analysis 46,47 . Notably, vimentin expression is negatively correlated with the expression of E‐cadherin in OSCC 46,47,56 . There has a study even suggested that combined E‐cadherin and vimentin expression as a measure of EMT showed a superior prognostic significance compared with individual protein expression 56 …”

Section: Alteration Of the Cytoskeletonmentioning

confidence: 99%

“…46,47,56 There has a study even suggested that combined E-cadherin and vimentin expression as a measure of EMT showed a superior prognostic significance compared with individual protein expression. 56 In addition to the above proteins, other cytoskeletal proteins are involved in EMT, including βIII-tubulin, acetylated tubulin and S100A4, which are considered as potential targets in OSCC therapy. [57][58][59] 4 | EMT-INDUCING TRANSCRIPTION FACTORS…”

Section: Vimentin Is a Type III Intermediate Filament Protein Constitmentioning

confidence: 99%

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Epithelial‐to‐mesenchymal transition in oral squamous cell carcinoma: Challenges and opportunities

Ling

Cheng

Tao

2020

Intl Journal of Cancer

146

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Oral squamous cell carcinoma (OSCC) is the most common malignancy representing 90% of all forms of oral cancer worldwide. Although great efforts have been made in the past decades, the 5‐year survival rate of OSCC patients is no more than 60% due to tumor metastasis and subsequent recurrence. The metastasis from the primary site is due to a complex process known as epithelial‐to‐mesenchymal transition (EMT). During the EMT, epithelial cells gradually acquire the structural and functional characteristics of mesenchymal cells, leading to the upregulation of cell migration and the promotion of tumor cell dissemination. Therefore, EMT attracted broad attention due to its close relationship with cancer invasion and metastasis. Therefore, in the present review, an extensive description of the current research on OSCC and the role of EMT in this cancer type is provided, including diverse EMT markers, regulatory networks and crucial EMT‐inducing transcription factors in OSCC. Moreover, a brief summary was made regarding the current application of EMT‐correlated indexes in the prognostic analysis of OSCC patients, and the potential therapeutic approaches against OSCC and difficulties in the development of an effective anti‐EMT treatment are discussed. Our aim is to provide novel insights to develop new strategies to combat OSCC by targeting EMT.

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Section: Alteration Of the Cytoskeletonmentioning

confidence: 99%

Section: Vimentin Is a Type III Intermediate Filament Protein Constitmentioning

confidence: 99%

Epithelial‐to‐mesenchymal transition in oral squamous cell carcinoma: Challenges and opportunities

Ling

Cheng

Tao

2020

Intl Journal of Cancer

146

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“…Vimentin is an important marker of EMT that has an crucial role in cancer metastasis. 35 MMP3 can degrade extracellular matrix to promote cancer progression including invasion and metastasis. 36,37 Here, we revealed for the first time that vimentin and MMP3 are target genes of miR-515-3p.…”

Section: Discussionmentioning

confidence: 99%

Identification of miR-515-3p and its targets, vimentin and MMP3, as a key regulatory mechanism in esophageal cancer metastasis: functional and clinical significance

Xu²,

Zhang

et al. 2020

Sig Transduct Target Ther

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Metastasis is the main factor of treatment failure in cancer patients, but the underlying mechanism remains to be elucidated and effective new treatment strategies are urgently needed. This study aims to explore novel key metastasis-related microRNAs (miRNAs) in esophageal squamous cell carcinoma (ESCC). By comparing miRNA profiles of the highly metastatic ESCC cell sublines, we established through serial in vivo selection with the parental cells, we found that the expression level of miR-515-3p was lower in ESCC tumor tissues than adjacent normal tissues, further decreased in metastatic tumors, and moreover, markedly associated with advanced stage, metastasis and patient survival. The in vitro and in vivo assays suggested that miR-515-3p could increase the expression of the epithelial markers as well as decrease the expression of the mesenchymal markers, and more importantly, suppress invasion and metastasis of ESCC cells. Mechanistically, we revealed that miR-515-3p directly regulated vimentin and matrix metalloproteinase-3 (MMP3) expression by binding to the coding sequence and 3′untranslated region, respectively. In addition, the data from whole-genome methylation sequencing and methylation-specific PCR indicated that the CpG island within miR-515-3p promoter was markedly hypermethylated in ESCC cell lines and ESCC tumor tissues, which may lead to deregulation of miR-515-3p expression in ESCC. Furthermore, our preclinical experiment provides solid evidence that systemic delivery of miR-515-3p oligonucleotide obviously suppressed the metastasis of ESCC cells in nude mice. Taken together, this study demonstrates that miR-515-3p suppresses tumor metastasis and thus represents a promising prognostic biomarker and therapeutic strategy in ESCC.

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“…Recent in vitro studies confirm the role of EMT markers in the progression of oral epithelial dysplasia. Dmello et al (46) evaluated the expression of vimentin in epithelial cells derived from OPMDs. In that study, the exogenous expression of vimentin contributed to the occurrence of EMT and subsequent malignant transformation.…”

Section: Discussionmentioning

confidence: 99%

Prognostic value of the immunohistochemical detection of epithelial-mesenchymal transition biomarkers in oral epithelial dysplasia: A systematic review

Morais

Pinheiro²,

Lira³

et al. 2020

Med Oral

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Background: Oral potentially malignant disorders (OPMDs) comprise a range of clinical-pathological alterations that are frequently characterized as architectural and cytological derangements upon histological analysis. Epithelial-mesenchymal transition (EMT) has been proposed as a critical mechanism for the acquisition of the malignant phenotype in neoplastic epithelial processes. This study aims to systematically review the current findings on the immunohistochemical expression of epithelial-mesenchymal transition markers in oral potentially malignant disorders and to evaluate their possible application as biomarkers associated with the progression of oral epithelial dysplasias. Material and Methods: A systematic search was performed in the following databases: PubMed, EMBASE, Chinese BioMedical Literature Database, and Cochrane Library. Articles that evaluated the relationship between the expression of EMT markers and the degree of oral epithelial dysplasia were selected for the systematic review. The quality of each eligible study was evaluated by independent reviewers that used operationalized prognostic biomarker reporting guidelines (REMARK). Results: Seventeen articles met all inclusion criteria and were selected. The EMT markers analyzed exhibited an important association with the prognosis of the cases evaluated. The results showed a progressive increase in the expression of nuclear transcription factors and markers of mesenchymal differentiation, as well as negative regulation of epithelial and cell adhesion markers, according to the stage of oral epithelial dysplasia.

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Aberrant expression of vimentin predisposes oral premalignant lesion derived cells towards transformation

Cited by 11 publications

References 22 publications

Epithelial‐to‐mesenchymal transition in oral squamous cell carcinoma: Challenges and opportunities

Epithelial‐to‐mesenchymal transition in oral squamous cell carcinoma: Challenges and opportunities

Identification of miR-515-3p and its targets, vimentin and MMP3, as a key regulatory mechanism in esophageal cancer metastasis: functional and clinical significance

Prognostic value of the immunohistochemical detection of epithelial-mesenchymal transition biomarkers in oral epithelial dysplasia: A systematic review

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