Aberrant histone methylation in mouse early preimplantation embryos derived from round spermatid injection

Ooga, Masatoshi; Kikuchi, Yasuyuki; Ito, Daiyu; Kazama, Kousuke; Inoue, Rei; Sakamoto, Mizuki; Wakayama, Sayaka; Wakayama, Teruhiko

doi:10.1016/j.bbrc.2023.09.020

Cited by 1 publication

(2 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, in addition to oogenesis, spermiogenesis is important for chromatin structure and associated gene expression in early preimplantation embryos. Recently, we attempted to correct inherited histone methylation in ROSI zygotes [ 67 ]. A previous study revealed that ROSI zygotes had abnormal DNA and H3K9me3 methylation and that Trichostatin A (TSA), a histone deacetylase inhibitor, could correct this DNA methylation abnormality, and not H3K9me3 [ 62 ].…”

Section: Paternal Contribution To the Zygotic Chromatin Structurementioning

confidence: 99%

“…Furthermore, H3K36me3 was identified as a novel aberrant histone abnormality in ROSI-zygotes and could not be corrected by TSA treatment of ROSI-zygotes, but rather enhanced abnormality. Collectively, our findings suggest that histone replacement during spermiogenesis is important for the establishment of a proper zygotic chromatin structure and that it is not easy to correct the abnormality once it has occurred by bypassing histone-protamine replacement during spermiogenesis [ 67 ].…”

Section: Paternal Contribution To the Zygotic Chromatin Structurementioning

confidence: 99%

See 1 more Smart Citation

Chromatin structure in totipotent mouse early preimplantation embryos

OOGA

2024

Journal of Reproduction and Development

Self Cite

View full text Add to dashboard Cite

Totipotency refers to the ability of a single cell to give rise to all the different cell types in the body. Terminally differentiated germ cells (sperm and oocytes) undergo reprogramming, which results in the acquisition of totipotency in zygotes. Since the 1990s, numerous studies have focused on the mechanisms of totipotency. With the emergence of the concept of epigenetic reprogramming, which is important for the undifferentiated and differentiated states of cells, the epigenomes of germ cells and fertilized oocytes have been thoroughly analyzed. However, in early immunostaining studies, detailed epigenomic information was difficult to obtain. In recent years, the explosive development of next-generation sequencing has made it possible to acquire genome-wide information and the rise of genome editing has facilitated the analysis of knockout mice, which was previously difficult. In addition, live imaging can effectively analyze zygotes and 2-cell embryos, for which the number of samples is limited, and provides biological insights that cannot be obtained by other methods. In this review, the progress of our research using these advanced techniques is traced back from the present to its earliest years.

show abstract

Section: Paternal Contribution To the Zygotic Chromatin Structurementioning

confidence: 99%

Section: Paternal Contribution To the Zygotic Chromatin Structurementioning

confidence: 99%