Aberrant lncRNA–mRNA expression profile and function networks during the adipogenesis of mesenchymal stem cells from patients with ankylosing spondylitis

Cen, Shuizhong; Cai, Mingxi; Wang, Yihan; Lu, Xiaoyan; Chen, Zhipeng; Chen, Haobo; Fang, Yingdong; Wu, Changping; Qiu, Sujun; Liu, Zhenhua

doi:10.3389/fgene.2022.991875

Cited by 6 publications

(5 citation statements)

References 39 publications

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“…The ceRNA network regulatory map was used to predict all possible interactions of the differential lncRNAs, miRNAs, and mRNAs, calculate the correlation of targeting relationships, and visualize them using Cytoscape (v3.8.2). The Coexpression Network (CN) assumes that co-expressed genomes are involved in the same pathway, regulated by the same stimuli, and have similar biological activity ( Cen et al, 2022 ). Based on the Pearson correlation values algorithm, lncRNAs and coding genes were identified.…”

Section: Methodsmentioning

confidence: 99%

Long non-coding RNA SNHG9 regulates viral replication in rhabdomyosarcoma cells infected with enterovirus D68 via miR-150-5p/c-Fos axis

et al. 2023

Front. Microbiol.

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BackgroundThe Enterovirus D68 (EV-D68) epidemic has increased knowledge of the virus as a pathogen capable of causing serious respiratory and neurological illnesses. It has been shown that long noncoding RNAs (lncRNAs) regulate viral replication and infection via multiple mechanisms or signaling pathways. However, the precise function of lncRNAs in EV-D68 infection remains unknown.MethodsThe differential expression profiles of lncRNA in EV-D68-infected and uninfected rhabdomyosarcoma (RD) cells were studied using high-throughput sequencing technology. The knockdown through small interfering RNA (siRNA) and overexpression of lncRNA SNHG9 (small ribonucleic acid host gene 9) were applied to investigate how lncRNA SNHG9 regulates EV-D68 propagation. The targeted interactions of lncRNA SNHG9 with miR-150-5p and miR-150-5p with c-Fos were validated using dual luciferase reporter system. LncRNA SNHG9 knockdown and miR-150-5p inhibitor were co-transfected with RD cells. QRT-PCR and western blot were used to detect RNA and protein levels, of c-Fos and VP1, respectively. Median tissue culture infectious dose (TCID50) was applied to detect viral titers.ResultsThe results demonstrated that a total of 375 lncRNAs were highly dysregulated in the EV-D68 infection model. In the EV-D68 infection model, lncRNA SNHG9 and c-Fos were increased in EV-D68-infected RD cells. However, the expression level of miR-150-5p was downregulated. In addition, overexpression of SNHG9 in RD cells resulted in decreased viral replication levels and viral titers following infection with EV-D68, and further experiments revealed that overexpression of SNHG9 inhibited the viral replication by targeting increased miR-150-5p binding and significantly increased c-Fos expression in RD cells.ConclusionOur findings indicate that the SNHG9/miR-150-5p/c-Fos axis influences EV-D68 replication in host cells and that SNHG9 may be a possible target for anti-EV-D68 infection therapies.

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Section: Methodsmentioning

confidence: 99%

Long non-coding RNA SNHG9 regulates viral replication in rhabdomyosarcoma cells infected with enterovirus D68 via miR-150-5p/c-Fos axis

et al. 2023

Front. Microbiol.

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“…Numerous lncRNAs have been documented to exert regulatory effects on the adipogenic differentiation process ( Squillaro et al, 2020 ). In a study by Cen S et al, the analysis of ASMSCs during adipogenesis in comparison to human-derived mesenchymal stem cells revealed 137 upregulated and 126 downregulated lncRNAs ( Cen et al, 2022 ). Notably, three of the top 10 differentially expressed lncRNAs (ENST00000429588.1, ENST00000400755.3, and ENST00000512300.1) exhibited significant co-expression with differentially expressed mRNAs.…”

Section: Lncrnas Involved In the Pathogenesis Of Asmentioning

confidence: 99%

A review of long non-coding RNAs in ankylosing spondylitis: pathogenesis, clinical assessment, and therapeutic targets

Wang,

Yang,

et al. 2024

Front. Cell Dev. Biol.

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Ankylosing spondylitis (AS) is a chronic immune-mediated type of inflammatory arthritis characterized by inflammation, bone erosion, and stiffness of the spine and sacroiliac joints. Despite great efforts put into the investigation of the disease, the pathogenesis of AS remains unclear, posing challenges in identifying ideal targets for diagnosis and treatment. To enhance our understanding of AS, an increasing number of studies have been conducted. Some of these studies reveal that long non-coding RNAs (lncRNAs) play crucial roles in the etiology of AS. Some certain lncRNAs influence the development of AS by regulating inflammatory responses, autophagy, apoptosis, and adipogenesis, as well as the proliferation and differentiation of cells. Additionally, some lncRNAs demonstrate potential as biomarkers, aiding in monitoring disease progression and predicting prognosis. In this review, we summarize recent studies concerning lncRNAs in AS to elucidate the underlying mechanisms in which lncRNAs are involved and their potential values as biomarkers for disease assessment and druggable targets for therapy.

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“…Cen et al ( 95 ) compared mesenchymal stem cells from patients with AS (ASMSCs) and healthy donors (HDMSCs) and found that HDMSCs had a stronger adipogenic differentiation ability. A total of 263 lncRNAs showed differential expression during adipogenesis in ASMSCs, and reverse transcription quantitative-polymerase chain reaction results showed that the expression of the top 10 lncRNAs was consistent with the high-throughput sequencing results.…”

Section: Research Progress On Lncrna Expression In Asmentioning

confidence: 99%

“…A total of 263 lncRNAs showed differential expression during adipogenesis in ASMSCs, and reverse transcription quantitative-polymerase chain reaction results showed that the expression of the top 10 lncRNAs was consistent with the high-throughput sequencing results. This is expected to provide new targets for future interventions in adipogenic metaplasia in AS ( 95 ). Another study found 200 upregulated and 70 downregulated lncRNAs in a comparison between patients with AS and healthy individuals ( 96 ).…”

Section: Research Progress On Lncrna Expression In Asmentioning

confidence: 99%

Research progress on long non‑coding RNAs in non‑infectious spinal diseases (Review)

Ma,

Liu,

Zhang

et al. 2024

Mol Med Rep

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Spinal diseases, including intervertebral disc degeneration (IDD), ankylosing spondylitis, spinal cord injury and other non-infectious spinal diseases, severely affect the quality of life of patients. Current treatments for IDD and other spinal diseases can only relieve symptoms and do not completely cure the disease. Therefore, there is an urgent need to explore the causes of these diseases and develop new treatment approaches. Long non-coding RNA (lncRNA), a form of non-coding RNA, is abundant in diverse sources, has numerous functions, and plays an important role in the occurrence and development of spinal diseases such as IDD. However, the mechanism of action of lncRNAs has not been fully elucidated, and significant challenges remain in the use of lncRNAs as new therapeutic targets. The present article reviews the sources, classification and functions of lncRNAs, and introduces the role of lncRNAs in spinal diseases, such as IDD, and their therapeutic potential.

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Aberrant lncRNA–mRNA expression profile and function networks during the adipogenesis of mesenchymal stem cells from patients with ankylosing spondylitis

Cited by 6 publications

References 39 publications

Long non-coding RNA SNHG9 regulates viral replication in rhabdomyosarcoma cells infected with enterovirus D68 via miR-150-5p/c-Fos axis

Long non-coding RNA SNHG9 regulates viral replication in rhabdomyosarcoma cells infected with enterovirus D68 via miR-150-5p/c-Fos axis

A review of long non-coding RNAs in ankylosing spondylitis: pathogenesis, clinical assessment, and therapeutic targets

Research progress on long non‑coding RNAs in non‑infectious spinal diseases (Review)

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