Aberrant methylation scanning by quantitative DNA melting analysis with hybridization probes as exemplified by liquid biopsy of SEPT9 and HIST1H4F in colorectal cancer

Botezatu, Irina V.; Kondratova, Valentina N.; Stroganova, Anna M.; Dranko, Svetlana L.; Lichtenstein, Anatoly V.

doi:10.1016/j.cca.2023.117591

Clinica Chimica Acta

2023

DOI: 10.1016/j.cca.2023.117591

|View full text |Cite

Aberrant methylation scanning by quantitative DNA melting analysis with hybridization probes as exemplified by liquid biopsy of SEPT9 and HIST1H4F in colorectal cancer

Irina V. Botezatu,

Valentina N. Kondratova,

Anna M. Stroganova

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2024

Publication Types

Select...

Article1

Relationship

Self Cite0

Independent1

Authors

Journals

Cited by 1 publication

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

LINE-1 hypomethylation and HIST1H4F hypermethylation as oncomarkers in liquid biopsy of colorectal cancer

Kondratova,

Botezatu,

Stroganova

et al. 2024

Usp. mol. onkol

View full text Add to dashboard Cite

Introduction. Local hypermethylation of gene promoters and global genome hypomethylation are well-known manifestations of aberrant methylation associated with carcinogenesis. We investigated this phenomenon as a possible diagnostic marker for liquid biopsy of colorectal cancer using the original quantitative DNA melting analysis with hybridiza-tion probes (qDMA-HP) method. Aim. To quantify the methylation of HIST1H4F promoter and LINE-1 transposon in circulating blood plasma DNA of colorectal cancer patients. Materials and methods. Bisulfite-treated DNA samples isolated from blood plasma of healthy donors and cancer patients were analyzed. HIST1H4F methylation was assessed by asymmetric polymerase chain reaction with hybridized probe and post-amplification melting of probe / amplicon hybrids. To test for repetitive and highly polymorphic LINE-1 sequences, asymmetric polymerase chain reaction with hybridized probe and SYBR Green intercalating dye was used, followed by melting of hybrids and analysis of multicomponent melt curves. Results. High diagnostic efficiency of LINE-1 and HIST1H4F methylation markers in liquid biopsy of colorectal cancer was demonstrated with the area under the ROC curve = 0.92, sensitivity – 100 %, specificity – 84 %. Cross validation supports this result. Hypermethylation of HIST1H4F and hypomethylation of LINE-1 are statistically significantly correlated (Spearman correlation coefficient r = 0.4; p = 0.01). Conclusion. The qDMA-HP is suitable for quantitative assessment of aberrant methylation of various clinically significant genes.

show abstract

LINE-1 hypomethylation and HIST1H4F hypermethylation as oncomarkers in liquid biopsy of colorectal cancer

Kondratova,

Botezatu,

Stroganova

et al. 2024

Usp. mol. onkol

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Aberrant methylation scanning by quantitative DNA melting analysis with hybridization probes as exemplified by liquid biopsy of SEPT9 and HIST1H4F in colorectal cancer

Cited by 1 publication

References 60 publications

<i>LINE-1</i> hypomethylation and <i>HIST1H4F</i> hypermethylation as oncomarkers in liquid biopsy of colorectal cancer

<i>LINE-1</i> hypomethylation and <i>HIST1H4F</i> hypermethylation as oncomarkers in liquid biopsy of colorectal cancer

Contact Info

Product

Resources

About