Abnormal Crystallization Sequence of Calcium Carbonate in the Presence of <i>Synechococcus</i> sp. PCC 7942

Yang, Guoguo; Li, Fu-Chun; Deng, Zelan; Wang, Yazhi; Su, Zhimeng; Huang, Lingjie; Yin, Ling; Ji, Chen

doi:10.1080/01490451.2022.2100948

Cited by 5 publications

(4 citation statements)

References 43 publications

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“…Additionally, cell viability of bioprinted PCC 7002 was also analyzed in mineralization medium, where it also was very high throughout the 14-day cultivation period (74.2–80.6%), confirming the stability of PCC 7002 against high salinities ( Ludwig and Bryant, 2012 ). Since the mineralization process of cyanobacteria is fast and takes place within a few days or even hours, the viability tested in this study is considered high enough for the fabrication of the LBM ( Heveran et al, 2020 ; Sidhu et al, 2022 ; Yang et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

3D bioprinting of mineralizing cyanobacteria as novel approach for the fabrication of living building materials

Reinhardt

Ihmann

Ahlhelm

et al. 2023

Front. Bioeng. Biotechnol.

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Living building materials (LBM) are gaining interest in the field of sustainable alternative construction materials to reduce the significant impact of the construction industry on global CO2 emissions. This study investigated the process of three-dimensional bioprinting to create LBM incorporating the cyanobacterium Synechococcus sp. strain PCC 7002, which is capable of producing calcium carbonate (CaCO3) as a biocement. Rheology and printability of biomaterial inks based on alginate-methylcellulose hydrogels containing up to 50 wt% sea sand were examined. PCC 7002 was incorporated into the bioinks and cell viability and growth was characterized by fluorescence microscopy and chlorophyll extraction after the printing process. Biomineralization was induced in liquid culture and in the bioprinted LBM and observed by scanning electron microscopy, energy-dispersive X-ray spectroscopy, and through mechanical characterization. Cell viability in the bioprinted scaffolds was confirmed over 14 days of cultivation, demonstrating that the cells were able to withstand shear stress and pressure during the extrusion process and remain viable in the immobilized state. CaCO3 mineralization of PCC 7002 was observed in both liquid culture and bioprinted LBM. In comparison to cell-free scaffolds, LBM containing live cyanobacteria had a higher compressive strength. Therefore, bioprinted LBM containing photosynthetically active, mineralizing microorganisms could be proved to be beneficial for designing environmentally friendly construction materials.

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Section: Discussionmentioning

confidence: 99%

3D bioprinting of mineralizing cyanobacteria as novel approach for the fabrication of living building materials

Reinhardt

Ihmann

Ahlhelm

et al. 2023

Front. Bioeng. Biotechnol.

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“…In our study, eCA activity was ~ 1.6-2.0 times higher during the exponential growth phase and reduced gradually through the stationary phase (Supplementary figure, Figure S1). Similarly, Yang et al (2023) measured the CA anhydrase in solution over a 30-day growth experiment with Synechococcus PCC 7942 and reported an increase over the lag phase and large fluctuations over the exponential phase. During the stationary phase, CA did not vary greatly but a minor decrease was recorded in the late stationary phase (Yang et al, 2023).…”

Section: Exponential Growth Phasementioning

confidence: 96%

“…Similarly, Yang et al (2023) measured the CA anhydrase in solution over a 30-day growth experiment with Synechococcus PCC 7942 and reported an increase over the lag phase and large fluctuations over the exponential phase. During the stationary phase, CA did not vary greatly but a minor decrease was recorded in the late stationary phase (Yang et al, 2023). In our study, the higher eCA activity recorded could explain the strongly stained ~45-47-kDa band that was only identified in our SDS-PAGE gels of EPS produced during the exponential phase (Figure 4, lanes 2-3).…”

Section: Exponential Growth Phasementioning

confidence: 96%

Properties of exopolymeric substances (EPS) produced during cyanobacterial growth: potential role in whiting events

Brito

Bundeleva

Marin

et al. 2023

Preprint

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Abstract. Extracellular polymeric substances (EPS) are an important organic carbon reservoir in many pelagic and benthic environments. The production of EPS is intimately associated with the growth of phyto- and picoplankton. EPS plays a critical role in carbonate precipitation through the binding of cations and by acting as a nucleation site for minerals. Large-scale episodes of fine-grained calcium carbonate precipitation in the water column (whiting events) have been linked to cyanobacterial blooms, including of Synechococcus spp.,. The mechanisms that trigger these precipitation events are still debated. We pose that the cyanobacterial EPS, produced during exponential and stationary growth phases plays a critical role in the formation of whitings. The aim of this study was to investigate the production of EPS during a two-month cyanobacterial growth, mimicking a bloom. We further evaluated the potential role of EPS in carbonate precipitation. The production and properties of EPS produced at different Synechococcus spp. growth stages were investigated and carbonate mineral formation within these EPS matrices was determined in forced precipitation experiments. EPS produced during the early and late stationary phase contained a larger amount of negatively charged groups than present in EPS produced during the exponential phase. Consequently, a higher Ca2+ binding affinity of the stationary phase-EPS led to the formation of a larger amount of smaller carbonate minerals (<50 µm) compared to crystals formed in exponential phase-EPS, which were less and larger (> 50 µm). These findings were used to establish a conceptual model for picoplankton bloom-mediated CaCO3 precipitation that can explain the role of EPS in whitings (see graphical abstract).

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“…Microbial mineralization occurs in various environments, including soil, freshwater, marine ecosystems, and oilfield wastewater. Environmental conditions play a crucial role in determining the specific type of calcium carbonate (CaCO 3 ) mineral formed through bacterial precipitation [23][24][25]. Since Synechocystis sp.…”

Section: Introductionmentioning

confidence: 99%

Effect of Magnesium and Ferric Ions on the Biomineralization of Calcium Carbonate Induced by Synechocystis sp. PCC 6803

Zhao,

Han,

Liang

et al. 2023

Minerals

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The discovery of cyanobacteria fossils in microbialite prompts the investigation of carbonate biomineralization using cyanobacteria. However, the impact of coexisting magnesium and iron in microbialite on carbonate biomineralization has been overlooked. Here, Synechocystis sp. PCC 6803 was used to induce calcium carbonate in the presence of coexisting magnesium and ferric ions. The findings demonstrate that cell concentration, pH, carbonic anhydrase activity, and carbonate and bicarbonate concentrations decreased with increasing concentrations of magnesium and calcium ions. Ferric ions yielded a contrasting effect. The levels of deoxyribonucleic acid, protein, polysaccharides, and humic substances in extracellular polymeric substances increased in the presence of separated or coexisting calcium, magnesium, and ferric ions. Magnesium ions inhibited calcium ion precipitation, whereas ferric ions exhibited the opposite effect. Protein secondary structures became more abundant and O-C=O and N-C=O contents increased with increasing ion concentrations by Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analyses. Scanning electron microscopy revealed that ferric ions lead to rougher surfaces and incomplete rhombohedral structures of calcite, whereas magnesium ions promoted greater diversity in morphology. Magnesium ions enhanced the incorporation of ferric ions. This work aims to further understand the effect of magnesium and ferric ions on calcium carbonate biomineralization induced by cyanobacteria.

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Abnormal Crystallization Sequence of Calcium Carbonate in the Presence of Synechococcus sp. PCC 7942

Cited by 5 publications

References 43 publications

3D bioprinting of mineralizing cyanobacteria as novel approach for the fabrication of living building materials

3D bioprinting of mineralizing cyanobacteria as novel approach for the fabrication of living building materials

Properties of exopolymeric substances (EPS) produced during cyanobacterial growth: potential role in whiting events

Effect of Magnesium and Ferric Ions on the Biomineralization of Calcium Carbonate Induced by Synechocystis sp. PCC 6803

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