Abnormal DNA repair activities in lymphocytes of workers exposed to 1,3-butadiene

Hallberg, Lance; Bechtold, William E.; Grady, James J.; Legator, Marvin S.; Au, William W.

doi:10.1016/s0921-8777(97)00004-9

Cited by 56 publications

(27 citation statements)

References 28 publications

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“…Furthermore, no increases in chromosome aberrations were found in the Texas facilities, although a "Challenge Assay," in which cells were exposed to gamma rays in G1 in vitro, was positive [29]. The latter could perhaps reflect the induction of abnormal DNA repair after exposure to BD [13].…”

Section: Discussionmentioning

confidence: 96%

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Lack of increased genetic damage in 1,3-butadiene-exposed Chinese workers studied in relation to EPHX1 and GST genotypes

Zhang

Hayes

Guo

et al. 2004

Mutation Research/Genetic Toxicology and Environmental Mutagene

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Abstract1,3-Butadiene (BD) is an important industrial chemical and pollutant. Its ability to induce genetic damage and cause hematological malignancies in humans is controversial. We have examined chromosome damage by fluorescence in situ hybridization (FISH) and mutations in the HPRT gene in the blood of Chinese workers exposed to BD. Peripheral blood samples were collected and cultured from 39 workers exposed to BD (median level 2 ppm, 6 h time-weighted average) and 38 matched controls in Yanshan, China. No difference in the level of aneuploidy or structural changes in chromosomes 1, 7, 8, and 12 was detected in metaphase cells from exposed subjects in comparison with matched controls, nor was there an increase in the frequency of HPRT mutations in the BDexposed workers. Because genetic polymorphisms in glutathione S-transferase (GST) enzymes and microsomal epoxide hydrolase (EPHX1) may affect the genotoxic effects of BD and its metabolites, we also related chromosome alterations and gene mutations to GSTT1, GSTM1 and EPHX1 genotypes. Overall, there was no effect of variants in these genotypes on numerical or structural changes in chromosomes 1, 7, 8 and 12 or on HPRT mutant frequency in relation to BD exposure, but the GST genotypes did influence background levels of both hyperdiploidy and HPRT mutant frequency. In conclusion, our data show no increase in chromosomal aberrations or HPRT mutations among workers exposed to BD, even in potentially susceptible genetic subgroups. The study is, however, quite small and the levels of BD exposure are not extremely high, but our findings in China do support those from a similar study conducted in the Czech Republic. Together, these studies suggest that low levels of occupational BD exposure do not pose a significant risk of genetic damage.

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Section: Discussionmentioning

confidence: 96%

“…To provide mechanistic information on the potential carcinogenicity of BD in humans, we [7][8][9] and others [10][11][12][13][14][15] have investigated genotoxic effects in BD-exposed workers. In a study at a polybutadiene production facility in China, we examined a broad spectrum of genotoxic and other potential effects of BD.…”

Section: Introductionmentioning

confidence: 99%

Lack of increased genetic damage in 1,3-butadiene-exposed Chinese workers studied in relation to EPHX1 and GST genotypes

Zhang

Hayes

Guo

et al. 2004

Mutation Research/Genetic Toxicology and Environmental Mutagene

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“…Leukocytes produce heat shock proteins (Haire et al 1988), antioxidant enzymes (Pereira et al 1999), and metallothioneins (Garte et al 1995, Yurkowi & Makhijani 1998. Leukocytes are also capable of DNA repair (Hallberg et al 1997) and have multidrug resistance transporters (Legrand et al 1996); both processes are of high adaptive value when cells are stressed by xenobiotic chemicals.…”

Section: Introductionmentioning

confidence: 99%

Variations of transcript profiles between sea otters Enhydra lutris from Prince William Sound, Alaska, and clinically normal reference otters

Miles¹,

Bowen²,

Ballachey³

et al. 2012

Mar. Ecol. Prog. Ser.

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“…They were determined in 13 studies. In 11 studies effects of exposure to PAHs in coke ovens (7), hydrocarbons (25), benzene (31,32), acrylonitrile together with dimethyl formamide (35), BD (37,39), pesticides in farmers (41), fluorides (47), cytostatics (49), and cosmic radiation (54) were observed. Only in two studies, greenhouse workers exposed to pesticides (44) and crews exposed to cosmic radiation (55), were no effects of exposure found.…”

Section: Discussionmentioning

confidence: 99%

“…Only in two studies, greenhouse workers exposed to pesticides (44) and crews exposed to cosmic radiation (55), were no effects of exposure found. Positive effects with exposure to BD (39) and pesticides (41) were observed using a challenge assay, indicating some abnormalities in the DNA repair response.…”

Section: Discussionmentioning

confidence: 99%

Molecular Epidemiology Studies on Occupational and Environmental Exposure to Mutagens and Carcinogens, 1997-1999

Srám¹,

Binková²

2000

Environmental Health Perspectives

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Molecular epidemiology is a new and evolving area of research, combining laboratory measurement of internal dose, biologically effective dose, biologic effects, and influence of individual susceptibility with epidemiologic methodologies. Biomarkers evaluated were selected according to basic scheme: biomarkers of exposure-metabolites in urine, DNA adducts, protein adducts, and Comet assay parameters; biomarkers of effect-chromosomal aberrations, sister chromatid exchanges, micronuclei, mutations in the hypoxanthine-guanine phosphoribosyltransferase gene, and the activation of oncogenes coding for p53 or p21 proteins as measured on protein levels; biomarkers of susceptibility-genetic polymorphisms of genes CYP1A1, GSTM1, GSTT1, NAT2. DNA adducts measured by 32P-postlabeling are the biomarker of choice for the evaluation of exposure to polycyclic aromatic hydrocarbons. Protein adducts are useful as a biomarker for exposure to tobacco smoke (4-aminobiphenyl) or to smaller molecules such as acrylonitrile or 1,3-butadiene. Of the biomarkers of effect, the most common are cytogenetic end points. Epidemiologic studies support the use of chromosomal breakage as a relevant biomarker of cancer risk. The use of the Comet assay and methods analyzing oxidative DNA damage needs reliable validation for human biomonitoring. Until now there have not been sufficient data to interpret the relationship between genotypes, biomarkers of exposure, and biomarkers of effect for assessing the risk of human exposure to mutagens and carcinogens. Key words: air pollution, benzene, biomarkers, coke oven, genotypes, occupational exposure, PAHs, pesticides, tobacco smoke. -Envwron Health Perspect 1 08(suppl 1):57-70 (2000). http.//ehpnetl. niehs. nih.gov/docs/2000/suppl-1/57-70sram/abstract. html A new and evolving area of research termed molecular epidemiology attempts to merge sophisticated and highly sensitive laboratory methods (many of them developed during the recent revolution in molecular biology) with analytical epidemiologic methods. Molecular epidemiology bridges basic research in molecular biology and studies of human cancer causation by combining laboratory measurement of internal dose, biologically effective dose, biologic effects, and the influence of individual susceptibility with epidemiologic methodologies (1). The most common view is that the approach represents a natural convergence of molecular biology and epidemiology (2).The number of biomarkers available for evaluating genetic and cancer risk in humans is quite large. Their utility-for human biomonitoring is suggested by the well-known paradigm of environmentally induced cancer, which represents end points for assessing the entire spectrum of human-genotoxicant interactions (3). These biomarkers begin with exposure and include absorption, metabolism, distribution, critical target interaction (i.e., DNA damage and repair), genetic changes, and finally, disease, which is the province of traditional epidemiology. The development of biomarkers has given rise to the field of ...

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Abnormal DNA repair activities in lymphocytes of workers exposed to 1,3-butadiene

Cited by 56 publications

References 28 publications

Lack of increased genetic damage in 1,3-butadiene-exposed Chinese workers studied in relation to EPHX1 and GST genotypes

Lack of increased genetic damage in 1,3-butadiene-exposed Chinese workers studied in relation to EPHX1 and GST genotypes

Variations of transcript profiles between sea otters Enhydra lutris from Prince William Sound, Alaska, and clinically normal reference otters

Molecular Epidemiology Studies on Occupational and Environmental Exposure to Mutagens and Carcinogens, 1997-1999

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