2007
DOI: 10.1182/blood-2006-11-060707
|View full text |Cite
|
Sign up to set email alerts
|

Abnormal hematopoiesis in Gab2 mutant mice

Abstract: Gab2 is an important adapter molecule for cytokine signaling. Despite its major role in signaling by receptors associated with hematopoiesis, the role of Gab2 in hematopoiesis has not been addressed. We report that despite normal numbers of peripheral blood cells, bone marrow cells, and c-Kit ؉ Lin ؊ Sca-1 ؉ (KLS) cells, Gab2-deficient hematopoietic cells are deficient in cytokine responsiveness. Significant reductions in the number of colonyforming units in culture (CFU-C) in the presence of limiting cytokine… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

3
48
0

Year Published

2010
2010
2022
2022

Publication Types

Select...
5
2

Relationship

1
6

Authors

Journals

citations
Cited by 47 publications
(51 citation statements)
references
References 51 publications
3
48
0
Order By: Relevance
“…49,50 Deficiency in Gab2 decreases mast cell development 28 and multilineage repopulating activity of HSCs. 29 In the present study we found that D61G mutation in the N-SH2 domain of Shp-2 greatly enhanced the interaction between Shp-2 and tyrosine phosphorylated Gab2 in IL-3 signaling ( Figure 5C). Elevated baseline and cytokine (IL-3 and GM-CSF)-stimulated differentiation of Ptpn11 D61G/ϩ myeloid progenitors ( Figure 6D-E, supplemental Figure 6B) and LSK cells ( Figure 7D) was corrected by deletion of Gab2 in vivo and in vitro.…”
Section: Discussionmentioning
confidence: 56%
See 1 more Smart Citation
“…49,50 Deficiency in Gab2 decreases mast cell development 28 and multilineage repopulating activity of HSCs. 29 In the present study we found that D61G mutation in the N-SH2 domain of Shp-2 greatly enhanced the interaction between Shp-2 and tyrosine phosphorylated Gab2 in IL-3 signaling ( Figure 5C). Elevated baseline and cytokine (IL-3 and GM-CSF)-stimulated differentiation of Ptpn11 D61G/ϩ myeloid progenitors ( Figure 6D-E, supplemental Figure 6B) and LSK cells ( Figure 7D) was corrected by deletion of Gab2 in vivo and in vitro.…”
Section: Discussionmentioning
confidence: 56%
“…Gab2 ϩ/Ϫ mice had been backcrossed with C57BL/6 mice at least 8 generations. 29 Ptpn11 D61G/ϩ mice were backcrossed with C57BL/6 mice for 3 generations and then used to cross Gab2 ϩ/Ϫ mice to generate Ptpn11 D61G/ϩ /Gab2 ϩ/Ϫ mice. Ptpn11 D61G/ϩ / Gab2 ϩ/Ϫ mice were intercrossed to produce various types of mice (4th generation backcross to the C57BL6 background) for this study.…”
Section: Micementioning
confidence: 99%
“…Ϫ/Ϫ mice have reduced numbers of mast cells (68) and exhibit abnormal allergic responses (21) and defective competitive BM repopulation (70). Similar to CSF-1-null and CSF-1R-null mice, Gab2…”
Section: Gab2mentioning
confidence: 99%
“…Gab1's role in the myeloid lineage is unknown, whereas Gab3 deletion has no effect on hematopoiesis (51). A study examining the combined effects of early acting cytokines in Gab2 Ϫ/Ϫ mice noted a reduction in colony-forming activity (70). By focusing on the MNP lineage, we uncovered an essential requirement for Gab2 in CSF-1-dependent MNP development.…”
Section: Bm Cells In Vitro Correlated With Fewer F4/80mentioning
confidence: 99%
See 1 more Smart Citation