Abnormal meiotic chromosome behavior and gametic variation induced by intersectional hybridization in Populus L.

Wang, Jun; You, Hanli; Tian, Jun; Wang, Yongfu; Liu, Minghu; Duan, Wula

doi:10.1007/s11295-015-0880-z

Cited by 11 publications

(7 citation statements)

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“…Meiotic abnormalities related to irregular chromosome segregation, such as precocious chromosome migration to the poles in metaphase I (up to 38.09%) and metaphase II (up to 3.85%), and laggard chromosomes at anaphase I and II (up to 24.65%), were also detected. All the abovementioned meiotic abnormalities could alter the regular chromosome segregation, and the chromosomes that ultimately cannot be incorporated into the telophase I and II nuclei, leading to the origin of micronuclei (Ortiz et al., 2011; Pagliarini, 2000; Seijo & Solis Neffa, 2006; Wang et al., 2015). In our analysis, one micronucleus was observed at anaphase I, telophase I, and metaphase II (up to 12.5%), and one to two micronuclei were observed at anaphase II and telophase II (up to 8.33%).…”

Section: Resultsmentioning

confidence: 99%

Occurrence of 2nmicrospore production in diploid interspecific hybrids between the wild parental species of peanut (Arachis hypogaeaL., Leguminosae) and its relevance in the genetic origin of the cultigen

et al. 2020

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Unreduced (2n) gametes are the driving force that leads to polyploidization of plants in nature and an important tool for ploidy breeding. Peanut (Arachis hypogaea L., Leguminosae) seems most likely to have originated by sexual polyploidization through interspecific hybridization between the wild diploid relatives—A. duranensis Krapov. & W.C. Greg. and A. ipaënsis Krapov. & W.C. Greg—and subsequent binding of the unreduced gametes in the hybrid. Therefore, aiming to contribute to understanding the event of polyploid origin of this important crop, we made diploid hybrids between A. duranensis and A. ipaënsis and then investigated the production of unreduced gametes in the hybrids by analyzing the size range of pollen, the constitution of the sporads, and the microsporogenesis process. The meiotic abnormalities found, such as persistent bridges, tripolar spindles, and cytomixis processes, would lead to the formation of restitution nuclei in both the first and second meiotic division. These failures in the regularity of meiosis lead to the formation of dyads and triads, and consequently to 2n pollen grains. These findings constitute the first evidence of the formation of unreduced gametes in intergenomic hybrids obtained between the wild progenitors of A. hypogaea and enable us to propose a model of origin of the crop through sexual polyploidization.

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Section: Resultsmentioning

confidence: 99%

Occurrence of 2nmicrospore production in diploid interspecific hybrids between the wild parental species of peanut (Arachis hypogaeaL., Leguminosae) and its relevance in the genetic origin of the cultigen

et al. 2020

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“…italica , abnormal meiotic phenomena such as multinuclei, multinucleoli, monads, dyads, and polyads have been observed [ 49 ]. Previous studies on diploid Populus hybrids also showed that hybridization tends to induce occurrence of meiotic abnormalities [ 45 , 50 ]. However, compared with the diploid species and hybrids of Populus , although meiosis in ‘Yinzhong’ showed similar abnormalities, the frequency of abnormalities was high in ‘Yinzhong’, suggesting that more abnormalities might be induced by its distant genomic composition and polyploidization.…”

Section: Discussionmentioning

confidence: 99%

“…Production of unreduced gametes has been reported in many species and hybrids of the genus Populus [ 44 , 45 , 50 – 54 ]. There are multiple cytological mechanisms of unreduced gamete formation, which have been reviewed frequently [ 55 , 56 ].…”

Section: Discussionmentioning

confidence: 99%

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Abnormal meiosis in an intersectional allotriploid of Populus L. and segregation of ploidy levels in 2x × 3x progeny

et al. 2017

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Triploid plants are usually highly aborted owing to unbalanced meiotic chromosome segregation, but limited viable gametes can participate in the transition to different ploidy levels. In this study, numerous meiotic abnormalities were found with high frequency in an intersectional allotriploid poplar (Populus alba × P. berolinensis ‘Yinzhong’), including univalents, precocious chromosome migration, lagging chromosomes, chromosome bridges, micronuclei, and precocious cytokinesis, indicating high genetic imbalance in this allotriploid. Some micronuclei trigger mini-spindle formation in metaphase II and participate in cytokinesis to form polyads with microcytes. Unbalanced chromosome segregation and chromosome elimination resulted in the formation of microspores with aneuploid chromosome sets. Fusion of sister nuclei occurs in microsporocytes with precocious cytokinesis, which could form second meiotic division restitution (SDR)-type gametes. However, SDR-type gametes likely contain incomplete chromosome sets due to unbalanced segregation of homologous chromosomes during the first meiotic division in triploids. Misorientation of spindles during the second meiotic division, such as fused and tripolar spindles with low frequency, could result in the formation of first meiotic division restitution (FDR)-type unreduced gametes, which most likely contain three complete chromosome sets. Although ‘Yinzhong’ yields 88.7% stainable pollen grains with wide diameter variation from 23.9 to 61.3 μm, the pollen viability is poor (2.78% ± 0.38). A cross of ‘Yinzhong’ pollen with a diploid female clone produced progeny with extensive segregation of ploidy levels, including 29 diploids, 18 triploids, 4 tetraploids, and 48 aneuploids, suggesting the formation of viable aneuploidy and unreduced pollen in ‘Yinzhong’. Individuals with different chromosome compositions are potential to analyze chromosomal function and to integrate the chromosomal dosage variation into breeding programs of Populus.

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“…It is necessary to further improve the variety 'Xiaohuyang-2'. Wang et al (2015) found that the microsporocyte meiosis of 'Xiaohuyang-2' is highly abnormal in chromosome pairing and appointment, causing sterile of gametes. It is necessary to induce amphidiploid induction from the distant hybrid 'Xiaohuyang-2'.…”

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In Vitro Amphidiploid Induction of A Distant Hybrid Populus Simonii × P. Euphratica Cv. ‘Xiaohuyang-2’ and its Effect on Plant Morphology and Anatomy

Zhang

Cui

et al. 2022

Preprint

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In plants, highly gametic sterility of distant hybrids usually restricts their utilization in breeding programs. Amphidiploid induction produced by somatic chromosome doubling of distant hybrids can effectively restore their gametic fertility. In this study, nodal-segment and leaf explants of a distant hybrid Populus simonii × P. euphratica cv. ‘Xiaohuyang-2’ were used to induce chromosome doubling with colchicine in vitro. Although chromosome doubling of the nodal-segment explants only produced mixoploids, the treatments of leaf explants on adventitious bud regeneration medium successfully produced 4 amphidiploids, which might be attributed to the direct organogenesis of the adventitious buds on the leaf explants. The highest amphidiploid induction frequency was 16.7%. Both the explant survival rate and polyploidization frequency were significantly affected by colchicine concentration and exposure time. The amphidiploid plants were significantly differed from the diploid and mixoploid plants on morphological and anatomical characteristics. They had larger, thicker, and greener leaves than the diploids and mixoploids. The changes of stomatal features also accompanied with increase of ploidy level. The induced amphidiploid plants of the distant hybrid ‘Xiaohuyang-2’ are expected to play important roles in breeding programs of Populus in future, which can be used as a bridge parent with ability of unreduced gamete formation to cross with fast-growth germplasms to produce triploids pyramiding desirable traits of fast growth, easy cutting propagation, and salt and drought tolerances.

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Abnormal meiotic chromosome behavior and gametic variation induced by intersectional hybridization in Populus L.

Cited by 11 publications

References 34 publications

Occurrence of 2nmicrospore production in diploid interspecific hybrids between the wild parental species of peanut (Arachis hypogaeaL., Leguminosae) and its relevance in the genetic origin of the cultigen

Occurrence of 2nmicrospore production in diploid interspecific hybrids between the wild parental species of peanut (Arachis hypogaeaL., Leguminosae) and its relevance in the genetic origin of the cultigen

Abnormal meiosis in an intersectional allotriploid of Populus L. and segregation of ploidy levels in 2x × 3x progeny

In Vitro Amphidiploid Induction of A Distant Hybrid Populus Simonii × P. Euphratica Cv. ‘Xiaohuyang-2’ and its Effect on Plant Morphology and Anatomy

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