Abnormal PMC microtubule distribution pattern and chromosome behavior resulted in low pollen fertility of an intersubspecific autotetraploid rice hybrid

He, Jin-Hua; Shahid, Muhammad Qasim; Chen, Zhixiong; Xing-an, Chen; Liu, Xiangdong; Lu, Yue

doi:10.1007/s00606-010-0386-y

Cited by 31 publications

(36 citation statements)

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“…Autotetraploid rice has four sets of homologous chromosomes, and chromosome behaviour is more complicated in autotetraploid rice than in the diploid counterpart. Abnormal chromosome behaviour is a major reason for pollen abortion in autotetraploid rice (Luan et al 2007, 2009; He et al 2011a, b; Wu et al 2014). There are many types of abnormal chromosome behaviour in the autotetraploid rice, including chromosome straggling and chromosome lagging (Wu et al 2014).…”

Section: Discussionmentioning

confidence: 99%

“…Autotetraploid rice has strong biological advantages over diploid rice, such as huge vegetative organs, high yield potential, and wide adaptability (Shahid et al 2011, 2012; Tu et al 2014; Yang et al 2014). However, low fertility is a major obstacle in the utilization of autotetraploid rice (He et al 2011a; Shahid et al 2013a; Wu et al 2015; Li et al 2017). To improve the fertility and yield of autotetraploid rice, it is of utmost importance to investigate pollen development and its molecular mechanism in this organism.…”

Section: Introductionmentioning

confidence: 99%

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Cytological and transcriptome analyses reveal abrupt gene expression for meiosis and saccharide metabolisms that associated with pollen abortion in autotetraploid rice

Chen

Shahid

et al. 2018

Mol Genet Genomics

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Autotetraploid rice is a useful germplasm that has four chromosome sets and strong biological advantages; however, low fertility limits its commercial utilization. Little information is available about the DNA variation and differential gene expressions associated with low fertility in autotetraploid rice. In the present study, 81 SNPs and 182 InDels were identified in T449 (an autotetraploid rice line with low fertility) compared to E249 (diploid counterpart) by whole-genome re-sequencing. We detected only three non-synonymous SNPs and six large-effect InDels, which were associated with three and six genes, respectively. A total of 75 meiosis-related differentially expressed genes were detected during the meiosis stage by transcriptome analysis, including OsMTOPVIB, which is essential for meiotic DSB formation, and OsMOF, which takes part in homologous chromosome pairing and synapsis. Approximately 20.69% lagging chromosome at metaphase I and 4.65% abnormal tetrad were observed in T449. Moreover, transcriptome analysis revealed down-regulation of a sucrose transporter (OsSUT5) and two monosaccharide transporters (OsMST1 and OsMST8) in T449 at the single microspore stage, and their expression levels were verified by qRT-PCR. Cytological observation of saccharide distribution showed abnormal accumulation of saccharides in T449 and the contents of fructose and glucose were markedly higher in T449 than E249 at the single microspore stage. Our results suggested that polyploidy not only induces abrupt expression changes in the meiosis-related genes that lead to abnormal chromosome behavior, but also causes changes in the saccharide distribution and expression patterns of saccharide-related genes, which jointly causes sterility in the autotetraploid rice.Electronic supplementary materialThe online version of this article (10.1007/s00438-018-1471-0) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cytological and transcriptome analyses reveal abrupt gene expression for meiosis and saccharide metabolisms that associated with pollen abortion in autotetraploid rice

Chen

Shahid

et al. 2018

Mol Genet Genomics

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“…Bright-field microscopy, 4',6-diamidino-2-phenylindole (DAPI) fluorescence staining, and laser capture of individual cells have made it possible to dissect these cells (Fujita et al, 2010;Tang et al, 2010;Yang et al, 2011). Using DAPI fluorescence staining and WE-CLSM techniques, we were able to obtain PMCs at precise stages of meiosis and identify genes that were differentially expressed during these stages in rice (Feng et al, 2001;Lu et al, 2002;Zhang et al, 2005Zhang et al, , 2006He et al, 2011aHe et al, , 2011bWu et al, 2014). Theoretically, the fact that the PSILs have the same genetic background, except at the Sa, Sb, and Sc loci, eliminates the effect of different parental genomes, thus providing insight into the contribution of IE, PE, and IPE but not hybridization of the whole genome on genome-wide expression patterns.…”

Section: Polyploidy Enhanced Epistatic Interactions Between Pollen Stmentioning

confidence: 99%

“…japonica) hybrids have biological advantages over diploid hybrids, such as greater adaptability and yield potential Shahid et al, 2011Shahid et al, , 2012Wu et al, 2013). However, because autotetraploid rice hybrids have low seed set, largely because of high pollen abortion rates, it is challenging to produce hybrids with commercially favorable characteristics (He et al, 2011a(He et al, , 2011b. The ability to develop high-yielding intersubspecific autotetraploid rice hybrids depends on a detailed understanding of the cytological and molecular mechanisms underlying pollen abortion in these lines (He et al, 2011b).…”

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confidence: 99%

“…Therefore, abnormal chromosome behavior was considered to be an important cause of pollen abortion in autotetraploid rice hybrids (Luan et al, , 2009He et al, 2011aHe et al, , 2011b. However, we found that interactions between hybrid pollen sterility loci, including Sa, Sb, and Sc, contributed greatly to low pollen fertility in autotetraploid rice hybrids (Zhao et al, 2006;He et al, 2011b).…”

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Polyploidy enhances F1 pollen sterility loci interactions that increase meiosis abnormalities and pollen sterility in autotetraploid rice

Shahid

Chen

et al. 2015

Plant Physiol.

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Intersubspecific autotetraploid rice (Oryza sativa ssp. indica 3 japonica) hybrids have greater biological and yield potentials than diploid rice. However, the low fertility of intersubspecific autotetraploid hybrids, which is largely caused by high pollen abortion rates, limits their commercial utility. To decipher the cytological and molecular mechanisms underlying allelic interactions in autotetraploid rice, we developed an autotetraploid rice hybrid that was heterozygous (S i S j ) at F 1 pollen sterility loci (Sa, Sb, and Sc) using near-isogenic lines. Cytological studies showed that the autotetraploid had higher percentages (.30%) of abnormal chromosome behavior and aberrant meiocytes (.50%) during meiosis than did the diploid rice hybrid control. Analysis of gene expression profiles revealed 1,888 genes that were differentially expressed between the autotetraploid and diploid hybrid lines at the meiotic stage, among which 889 and 999 were up-and down-regulated, respectively. Of the 999 down-regulated genes, 940 were associated with the combined effect of polyploidy and pollen sterility loci interactions (IPE). Gene Ontology enrichment analysis identified a prominent functional gene class consisting of seven genes related to photosystem I (Gene Ontology 0009522). Moreover, 55 meiosis-related or meiosis stage-specific genes were associated with IPE in autotetraploid rice, including Os02g0497500, which encodes a DNA repair-recombination protein, and Os02g0490000, which encodes a component of the ubiquitin-proteasome pathway. These results suggest that polyploidy enhances epistatic interactions between alleles of pollen sterility loci, thereby altering the expression profiles of important meiosis-related or meiosis stage-specific genes and resulting in high pollen sterility.

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Photosynthetic Physiology and Molecular Response Mechanisms of Indica–Japonica Intersubspecific Tetraploid Rice Seedlings to Ion Beams

Huang

Jinzhe

et al. 2020

J Plant Growth Regul

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Abnormal PMC microtubule distribution pattern and chromosome behavior resulted in low pollen fertility of an intersubspecific autotetraploid rice hybrid

Cited by 31 publications

References 20 publications

Cytological and transcriptome analyses reveal abrupt gene expression for meiosis and saccharide metabolisms that associated with pollen abortion in autotetraploid rice

Cytological and transcriptome analyses reveal abrupt gene expression for meiosis and saccharide metabolisms that associated with pollen abortion in autotetraploid rice

Polyploidy enhances F1 pollen sterility loci interactions that increase meiosis abnormalities and pollen sterility in autotetraploid rice

Photosynthetic Physiology and Molecular Response Mechanisms of Indica–Japonica Intersubspecific Tetraploid Rice Seedlings to Ion Beams

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