Abnormal protein expression profiles in human ovaries from polycystic ovary syndrome patients

Jin, Min; Cai, Jie; Lu, Xiu-Min; Luo, Qing; Huang, Hua

doi:10.1016/j.fertnstert.2007.07.630

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2H), which may explain abnormalities in ovulation. Jin et al found that CTSD levels decreased significantly in the ovaries of patients with PCOS [76], which is consistent with the regulatory mode of CTSA in IL chickens. Other members of the cathepsin family, CTSH and CTSC, were both highly expressed in IL_ F1 cells (Fig.…”

Section: Proteases Play a Role In Chicken Ovulationmentioning

confidence: 55%

Proteo-transcriptomic profiles reveal key regulatory pathways and functions of LDHA in the ovulation of domestic chickens (Gallus gallus)

Nie,

Zhang,

Tian

et al. 2024

J Animal Sci Biotechnol

View full text Add to dashboard Cite

Background In poultry, the smooth transition of follicles from the preovulatory-to-postovulatory phase impacts egg production in hens and can benefit the poultry industry. However, the regulatory mechanism underlying follicular ovulation in avians is a complex biological process that remains unclear. Results Critical biochemical events involved in ovulation in domestic chickens (Gallus gallus) were evaluated by transcriptomics, proteomics, and in vitro assays. Comparative transcriptome analyses of the largest preovulatory follicle (F1) and postovulatory follicle (POF1) in continuous laying (CL) and intermittent laying (IL) chickens indicated the greatest difference between CL_F1 and IL_F1, with 950 differentially expressed genes (DEGs), and the smallest difference between CL_POF1 and IL_POF1, with 14 DEGs. Additionally, data-independent acquisition proteomics revealed 252 differentially abundant proteins between CL_F1 and IL_F1. Perivitelline membrane synthesis, steroid biosynthesis, lysosomes, and oxidative phosphorylation were identified as pivotal pathways contributing to ovulation regulation. In particular, the regulation of zona pellucida sperm-binding protein 3, plasminogen activator, cathepsin A, and lactate dehydrogenase A (LDHA) was shown to be essential for ovulation. Furthermore, the inhibition of LDHA decreased cell viability and promoted apoptosis of ovarian follicles in vitro. Conclusions This study reveals several important biochemical events involved in the process of ovulation, as well as crucial role of LDHA. These findings improve our understanding of ovulation and its regulatory mechanisms in avian species.

show abstract

Section: Proteases Play a Role In Chicken Ovulationmentioning

confidence: 55%