Abnormalities in sexual development and pollinator limitation in Michelia coriacea (Magnoliaceae), a critically endangered endemic to Southeast Yunnan, China

Zhao, Xing; Sun, Weibang

doi:10.1016/j.flora.2008.07.001

Cited by 18 publications

(11 citation statements)

References 28 publications

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“…Labeled fruits were harvested one month after the pollination treatment, in early September of each year and viable seeds were distinguished from aborted ones by the color of the aril (blood red vs. white or green, respectively) ( Zhao and Sun, 2009 ). The effect of pollination treatments was analyzed for two traits: fruit set [(number of fruits/number of tested flowers) × 100] and seed set [(number of viable seeds/number of initial ovules) × 100] by One-Way ANOVA using SPSS 16.0.…”

Section: Methodsmentioning

confidence: 99%

Floral characteristics and pollination ecology of Manglietia ventii (Magnoliaceae), a plant species with extremely small populations (PSESP) endemic to South Yunnan of China

Wang

Chen

et al. 2017

Plant Diversity

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Manglietia ventii is a highly endangered plant species endemic to Yunnan province in China, where there are only five known small populations. Despite abundant flowering there is very low fruit and seed set, and very few seedlings in natural populations, indicating problems with reproduction. The causes of low fecundity in M. ventii are not known, largely because of insufficient knowledge of the species pollination ecology and breeding system. We conducted observations and pollination experiments, and analyzed floral scents to understand the pollinator–plant interactions and the role of floral scent in this relationship, as well as the species breeding system. Like the majority of Magnoliaceae, M. ventii has protogynous and nocturnal flowers that emit a strong fragrance over two consecutive evenings. There is a closing period (the pre-staminate stage) during the process of anthesis of a flower, and we characterize the key flowering process as an “open-close-reopen” flowering rhythm with five distinct floral stages observed throughout the floral period of this species: pre-pistillate, pistillate, pre-staminate, staminate, and post-staminate. Flowers are in the pistillate stage during the first night of anthesis and enter the staminate stage the next night. During anthesis, floral scent emission occurs in the pistillate and staminate stages. The effective pollinators were weevils (Sitophilus sp.) and beetles (Anomala sp.), while the role of Rove beetles (Aleochara sp.) and thrips (Thrips sp.) in pollination of M. ventii appears to be minor or absent. The major chemical compounds of the floral scents were Limonene, β-Pinene, α-Pinene, 1,8-Cineole, Methyl-2-methylbutyrate, p-Cymene, Methyl-3-methyl-2-butenoate and 2-Methoxy-2-methyl-3-buten, and the relative proportions of these compounds varied between the pistillate and staminate stages. Production of these chemicals coincided with flower visitation by weevils and beetles. The results of pollination experiments suggest that M. ventii is pollinator-dependent, and low seed set in natural populations is a result of insufficient pollen deposition. Thus, conservation of the species should focus on improving pollination service through the introduction of genetically variable individuals and increase in density of reproducing trees.

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Section: Methodsmentioning

confidence: 99%

Floral characteristics and pollination ecology of Manglietia ventii (Magnoliaceae), a plant species with extremely small populations (PSESP) endemic to South Yunnan of China

Wang

Chen

et al. 2017

Plant Diversity

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“…Although the six sampled M. shiluensis groups formed a single large population comprising several smaller subpopulations [63], the genetic diversity in each subpopulation was relatively high. The reasons for high genetic diversity may be as follows: first, we speculate that gene exchange between M. shiluensis populations has historically been frequent, and thus, the existing population inherited rich ancestral genetic variation [64]; second, M. shiluensis is a perennial plant, and therefore, the recent sharp decline of individuals has not increased the chance of inbreeding, which reduces genetic diversity [65]; third, plants of the Michelia genus tend to cross-pollinate, which can reduce the loss of genetic diversity through large gene flows [55,66,67].…”

Section: High Level Of Genetic Diversitymentioning

confidence: 99%

High Genetic Diversity and Low Differentiation in Michelia shiluensis, an Endangered Magnolia Species in South China

Deng

Liu

Xie

et al. 2020

Forests

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Research Highlights: This study is the first to examine the genetic diversity of Michelia shiluensis (Magnoliaceae). High genetic diversity and low differentiation were detected in this species. Based on these results, we discuss feasible protection measures to provide a basis for the conservation and utilization of M. shiluensis. Background and Objectives: Michelia shiluensis is distributed in Hainan and Guangdong province, China. Due to human disturbance, the population has decreased sharply, and there is thus an urgent need to evaluate genetic variation within this species in order to identify an optimal conservation strategy. Materials and Methods: In this study, we used eight nuclear single sequence repeat (nSSR) markers and two chloroplast DNA (cpDNA) markers to assess the genetic diversity, population structure, and dynamics of 78 samples collected from six populations. Results: The results showed that the average observed heterozygosity (Ho), expected heterozygosity (He), and percentage of polymorphic loci (PPL) from nSSR markers in each population of M. shiluensis were 0.686, 0.718, and 97.92%, respectively. For cpDNA markers, the overall haplotype diversity (Hd) was 0.674, and the nucleotide diversity was 0.220. Analysis of markers showed that the genetic variation between populations was much lower based on nSSR than on cpDNA (10.18% and 77.56%, respectively, based on an analysis of molecular variance (AMOVA)). Analysis of the population structure based on the two markers shows that one of the populations (DL) is very different from the other five. Conclusions: High genetic diversity and low population differentiation of M. shiluensis might be the result of rich ancestral genetic variation. The current decline in population may therefore be due to human disturbance rather than to inbreeding or genetic drift. Management and conservation strategies should focus on maintaining the genetic diversity in situ, and on the cultivation of seedlings ex-situ for transplanting back to their original habitat.

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“…M. coriacea has been recently evaluated as a critically endangered tree based on IUCN criteria (Cicuzza et al 2007). As the species could bear abounding flowers almost every year, but its fruit and seed set are very low in the field, and thus the process of megaspore and microspore genesis and the development of gametophytes were recently observed (Zhao et al 2008;Zhao and Sun 2009). As a critically endangered plant, the effective and long-team conservation is urgently needed.…”

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confidence: 99%

Isolation and characterization of 12 microsatellite loci for Michelia coriacea (Magnoliaceae), a critically endangered endemic to Southeast Yunnan, China

et al. 2009

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By using a modified biotin-streptavidin capture method, a total of 12 microsatellite loci were developed and characterized for Michelia coriacea (Magnoliaceae), a critically endangered endemic to Southeast Yunnan, China. The number of alleles (A) ranged from two to six in 30 samples of this species. The ranges of observed (H O ) and expected (H E ) heterozygosities were 0.033-0.8000 and 0.033-0.7910, respectively. Cross-species amplification in M. yunnanensis showed that a subset of these markers holds promise for congeneric species. These polymorphic SSR markers would be useful tools for population genetics studies on M. coriacea and other congeneric species.

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Abnormalities in sexual development and pollinator limitation in Michelia coriacea (Magnoliaceae), a critically endangered endemic to Southeast Yunnan, China

Cited by 18 publications

References 28 publications

Floral characteristics and pollination ecology of Manglietia ventii (Magnoliaceae), a plant species with extremely small populations (PSESP) endemic to South Yunnan of China

Floral characteristics and pollination ecology of Manglietia ventii (Magnoliaceae), a plant species with extremely small populations (PSESP) endemic to South Yunnan of China

High Genetic Diversity and Low Differentiation in Michelia shiluensis, an Endangered Magnolia Species in South China

Isolation and characterization of 12 microsatellite loci for Michelia coriacea (Magnoliaceae), a critically endangered endemic to Southeast Yunnan, China

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