Abrupt Increase in the Level of Hydrogen Peroxide in Leaves of Winter Wheat Is Caused by Cold Treatment

Okuda, Tohru; Matsuda, Yusuke; Yamanaka, Akira; Sagisaka, Shonosuke

doi:10.1104/pp.97.3.1265

Cited by 408 publications

(206 citation statements)

References 10 publications

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“…The H 2 O 2 content was determined according to Okuda et al 50 by grinding leaf samples in ice-cold 200 ml of perchloric acid. After centrifugation at 1,200x g for 10 min, perchloric acid of the supernatant was neutralized with 4 M KOH.…”

Section: Methodsmentioning

confidence: 99%

Differential cadmium stress tolerance in five Indian mustard (Brassica junceaL.) cultivars

Gill

Khan

Tuteja

2011

Plant Signaling & Behavior

144

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IntroductionAgricultural soils worldwide are slightly to moderately contaminated with toxic heavy metals that restrict the crop plants to reach their full genetic potential and cause significant loss by reducing the crop productivity. 1 Among heavy metals, Cd is a non-essential and most deleterious heavy metal pollutant commonly released into the arable soil from various industrial, mining and farming practices, 2 and has been ranked No. 7 among the top 20 toxins which affect the human health by entering in the food chain. 3,4 Although Cd is a highly phytotoxic metal, it is easily taken up by plant roots growing on Cd-contaminated soils and transported to above ground plant parts. 5-7 The regulatory limit of Cd in agricultural soils is 100 mg Cd kg -1 soil, 8 but this threshold is continuously increasing because of anthropogenic and industrial activities. Plants growing on Cd contaminated soil result in Cd accumulation in all plant parts, which inhibits plant growth, affect nutrient uptake, alters the chloroplast ultrastructure, inactivates enzymes of CO 2 fixation, inhibits photosynthesis and induces lipid peroxidation and antioxidant machinery. 4,[9][10][11] However, Cd is a non redox-active metal, but it induces the generation of reactive oxygen species (ROS) including superoxide radical (O 2•-), hydrogen peroxide (H 2 O 2 ) and hydroxyl radicalThe presence of cadmium (cd) in the agricultural soils affects horticultural cultivars and constrains the crop productivity. a pot experiment was performed using five cultivars of mustard (Brassica juncea L.) to evaluate the difference in their response to cd toxicity under greenhouse conditions. The pots containing reconstituted soil were supplied with different concentration of cdcl 2 (0, 25, 50, 100 or 150 mg cd kg -1 soil). Increasing concentration of cd in the soil resulted in decreased growth, photosynthesis and yield. Maximum significant reduction in growth, photosynthesis and yield were observed with 150 mg cd kg -1 soil in all the cultivars. Our results indicate that the cultivar alankar is found to be more tolerant to cd stress, recording higher plant dry mass, net photosynthesis rate, associated with high antioxidant activity and low cd content in the plant leaves and thus less oxidative damage. cultivar rh30 experienced maximum damage in terms of reduction in growth, photosynthesis, yield characteristics and oxidative damage and emerged as sensitive cultivar. The data of tolerance index of alankar were found to be higher among all tested mustard cultivars which indicate its higher tolerance to cd. Better coordination of antioxidants protected alankar from cd toxicity, whereas lesser antioxidant activity in rh30 resulted in maximum damage. cultivars of mustard were ranked with respect to their tolerance to cd: alankar > Varuna > Pusa Bold > Sakha > rh30, respectively.Differential cadmium stress tolerance in five indian mustard (Brassica juncea L.) cultivars

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Section: Methodsmentioning

confidence: 99%

Differential cadmium stress tolerance in five Indian mustard (Brassica junceaL.) cultivars

Gill

Khan

Tuteja

2011

Plant Signaling & Behavior

144

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“…Subsequently, 1 mL trichloroacetic acid (w/v) was added, centrifuged for 10 min at 3,000 × g, and the absorbance of the supernatant was measured at a wavelength of 545 nm. 16 : 0.8 mL 12.5 mM 3-dimethylaminobenzoic acid (adjusted with 0.375 M phosphate buffer at pH 6.5), 160 μL 1.4 mM 3-methyl-2-benzo-thiazolinone hydrazone, and 40 μL 0.25 units peroxidase were added to a 2 mL flow-through collected in the analysis of the amount of O 2˙¯, incubated for 10 min at 25℃, and absorbance was measured at a wavelength of 590 nm.…”

Section: Measurement and Analysismentioning

confidence: 99%

Ameliorated Effect of Shading Treatment in Strong Light Period on Senescence and Photosynthesis in Cucumber ^|^lsquo;Jinchun No. 5^|^rsquo; Seedlings

Li¹,

Nishimura²,

Zhao³

et al. 2013

JARQ

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“…The content of H 2 O 2 was determined following the method of Okuda et al 51 Fresh leaf tissues (50 mg) were ground in an ice cold 200 mM perchloric acid. After centrifugation at 1200 £ g for 10 min, perchloric acid of the supernatant was neutralized with 4 M KOH.…”

Section: Estimation Of Content Of Ions Lipid Peroxidation and H 2 O mentioning

confidence: 99%

Exogenous salicylic acid improves photosynthesis and growth through increase in ascorbate-glutathione metabolism and S assimilation in mustard under salt stress

Nazar

Umar

Khan

2015

Plant Signaling & Behavior

202

114

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Ascorbate (AsA)-glutathione (GSH) cycle metabolism has been regarded as the most important defense mechanism for the resistance of plants under stress. In this study the influence of salicylic acid (SA) was studied on ascorbateglutathione pathway, S-assimilation, photosynthesis and growth of mustard (Brassica juncea L.) plants subjected to 100 mM NaCl. Treatment of SA (0.5 mM) alleviated the negative effects of salt stress and improved photosynthesis and growth through increase in enzymes of ascorbate-glutathione pathway which suggest that SA may participate in the redox balance under salt stress. The increase in leaf sulfur content through higher activity of ATP sulfurylase (ATPS) and serine acetyl transferase (SAT) by SA application was associated with the increased accumulation of glutathione (GSH) and lower levels of oxidative stress. These effects of SA were substantiated by the findings that application of SAanalog, 2,6, dichloro-isonicotinic acid (INA) and 1 mM GSH treatment produced similar results on rubisco, photosynthesis and growth of plants establishing that SA application alleviates the salt-induced decrease in photosynthesis mainly through inducing the enzyme activity of ascorbate-glutathione pathway and increased GSH production. Thus, SA/GSH could be a promising tool for alleviation of salt stress in mustard plants.

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Abrupt Increase in the Level of Hydrogen Peroxide in Leaves of Winter Wheat Is Caused by Cold Treatment

Cited by 408 publications

References 10 publications

Differential cadmium stress tolerance in five Indian mustard (Brassica junceaL.) cultivars

Differential cadmium stress tolerance in five Indian mustard (Brassica junceaL.) cultivars

Ameliorated Effect of Shading Treatment in Strong Light Period on Senescence and Photosynthesis in Cucumber ^|^lsquo;Jinchun No. 5^|^rsquo; Seedlings

Exogenous salicylic acid improves photosynthesis and growth through increase in ascorbate-glutathione metabolism and S assimilation in mustard under salt stress

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