Abscisic acid and salinity stress induced somaclonal variation and increased histone deacetylase (HDAC) activity in Ananas comosus var. MD2

Halim, Nur Asniyati Abdul; Tan, Boon Chin; Midin, Mohd Razik; Madon, M.; Khalid, Noorzulaani; Yaacob, Jamilah Syafawati

doi:10.1007/s11240-017-1367-4

Cited by 16 publications

(11 citation statements)

References 58 publications

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“…Notably, the explants cultured on different media displayed a reduced acitvity of deacetylases and acetylases, thereby suggesting that in vitro culture conditions per se might account for this result. Although, to the best of our knowledge, data on the acetylase/deacetylase activity during an in vitro culture are not available, the modulated expression of the specific HDAC and HAT genes in response to stress (reviewed in Chinnusamy and Zhu, 2009 ), which is inevitably associated with the explant culture together with reports on somaclonal epigenetic variants that have a deregulated deacetylase activity ( Yaacob et al, 2013 ; Halim et al, 2017 ) provide indirect support on the possible impact of in vitro culture conditions on the (de)acetylase activity.…”

Section: Discussionmentioning

confidence: 99%

Trichostatin A Triggers an Embryogenic Transition in Arabidopsis Explants via an Auxin-Related Pathway

et al. 2018

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Auxin is an important regulator of plant ontogenies including embryo development and the exogenous application of this phytohormone has been found to be necessary for the induction of the embryogenic response in plant explants that have been cultured in vitro. However, in the present study, we show that treatment of Arabidopsis explants with trichostatin A (TSA), which is a chemical inhibitor of histone deacetylases, induces somatic embryogenesis (SE) without the exogenous application of auxin. We found that the TSA-treated explants generated somatic embryos that developed efficiently on the adaxial side of the cotyledons, which are the parts of an explant that are involved in auxin-induced SE. A substantial reduction in the activity of histone deacetylase (HDAC) was observed in the TSA-treated explants, thus confirming a histone acetylation-related mechanism of the TSA-promoted embryogenic response. Unexpectedly, the embryogenic effect of TSA was lower on the auxin-supplemented media and this finding further suggests an auxin-related mechanism of TSA-induced SE. Congruently, we found a significantly increased content of indolic compounds, which is indicative of IAA and an enhanced DR5::GUS signal in the TSA-treated explants. In line with these results, two of the YUCCA genes (YUC1 and YUC10), which are involved in auxin biosynthesis, were found to be distinctly up-regulated during TSA-induced SE and their expression was colocalised with the explant sites that are involved in SE. Beside auxin, ROS were extensively accumulated in response to TSA, thereby indicating that a stress-response is involved in TSA-triggered SE. Relevantly, we showed that the genes encoding the transcription factors (TFs) that have a regulatory function in auxin biosynthesis including LEC1, LEC2, BBM, and stress responses (MYB118) were highly up-regulated in the TSA-treated explants. Collectively, the results provide several pieces of evidence about the similarities between the molecular pathways of SE induction that are triggered by TSA and 2,4-D that involve the activation of the auxin-responsive TF genes that have a regulatory function in auxin biosynthesis and stress responses. The study suggests the involvement of histone acetylation in the auxin-mediated release of the embryogenic program of development in the somatic cells of Arabidopsis.

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Section: Discussionmentioning

confidence: 99%

Trichostatin A Triggers an Embryogenic Transition in Arabidopsis Explants via an Auxin-Related Pathway

et al. 2018

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“…Extraction of RNA, cDNA synthesis and RT-PCR analysis were conducted based on previously described protocols (Halim et al 2018). To summarize, the total RNA was extracted from approximately 100 mg of leaf samples by using RNeasy Plant Mini kit (Qiagen, Germany), following the manufacturer's protocol.…”

Section: Quantitative Rt-pcr Analysismentioning

confidence: 99%

“…Previously, we have reported that the supplementation of high cytokinin concentration (4 mg•L -1 BAP plus 2 mg•L -1 IBA), as well as the exposure to salinity and abscisic acid (ABA), resulted in the production of dwarf pineapple variants (Halim et al 2018). The generated dwarf plantlets exhibited higher HDAC enzyme activity compared to non-dwarf plantlets, and their capability to revert to non-dwarf phenotype suggested that this somaclonal variation occurrence might be due to epigenetic modulation.…”

Section: Introductionmentioning

confidence: 99%

HD2 overexpression induces dwarfism and elevated histone deacetylase activities in transgenic Ananas comosus variety MD2

et al. 2022

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Plant tissue culture has served as one of the most efficient biotechnological tools to mass produce high quality major crops.Nevertheless, the use of this technique can result in the occurrence of somaclonal variation, which may be brought upon by genetic and/or epigenetic modulations. The epigenetic modulation of plant growth, particularly on the importance of histone deacetylation/acetylation on genome stability, transcriptional regulation, as well as plant development, has been reported in several species such as Arabidopsis, rice, and maize. However, the information on its role in regulating the growth of major crops such as pineapple is still lacking. Previously, we had reported that supplementation of high cytokinin in the tissue culture media and exposure to salinity and abscisic acid (ABA) resulted in the production of somaclonal variants that exhibited dwarf phenotypes, and that these occurrences were associated with epigenetic modulation via the involvement of histone deacetylases (HDACs). Thus, in the present study, we aimed to further elucidate and verify the involvement of histone deacetylation in the occurrence of dwarfism in tissue culture-derived pineapple plants, through HD2 gene overexpression. AtHD2 gene from Arabidopsis thaliana was synthesized and expressed in phenotypically normal Ananas comosus. Transformation efficiency was also studied, and application of 200-µM acetosyringone was observed to be more efficient than 500 µM vanillin by 10%. The transgenic plants were observed to exhibit dwarfed phenotypes and showed significantly high HDAC enzyme activities. Ploidy evaluation of the transgenic plants showed no ploidy change had occurred in the samples and remained as 2n. The relative expression level of AtHD2 was also higher (3.2-fold up-regulation) in the transgenic plants compared to the wild type plants. Collectively, these results indicate that HDACs indeed played an essential role in the response of A. comosus towards abiotic stress, and that this phenomenon is epigenetically regulated.

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“…2016; Gerszberg and Hnatuszko-Konka 2017). Somaclonal variations are obtained by treating plant hormone, sodium chloride (NaCl), and abscisic acid ABA to develop advantageous polygenetic traits to confer plant tolerance to abiotic stress (Halim et al 2017). In tomato plant, the selection of somaclonal variants in vitro in the form of protoplast, cell colony, or callus and even complete plants becomes possible.…”

Section: In Vitro Production Of Salinity Tolerant Tomatomentioning

confidence: 99%

Ethylene mediated physiological response for in vitro development of salinity tolerant tomato

Rao

Ansari

Singh

et al. 2020

Journal of Plant Interactions

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Abscisic acid and salinity stress induced somaclonal variation and increased histone deacetylase (HDAC) activity in Ananas comosus var. MD2

Cited by 16 publications

References 58 publications

Trichostatin A Triggers an Embryogenic Transition in Arabidopsis Explants via an Auxin-Related Pathway

Trichostatin A Triggers an Embryogenic Transition in Arabidopsis Explants via an Auxin-Related Pathway

HD2 overexpression induces dwarfism and elevated histone deacetylase activities in transgenic Ananas comosus variety MD2

Ethylene mediated physiological response for in vitro development of salinity tolerant tomato

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