Abscisic Acid Levels during Early Seed Development in <i>Sechium edule</i> Sw

Vernieri, Paolo; Perata, Pierdomenico; Lorenzi, Roberto; Ceccarelli, Nello

doi:10.1104/pp.91.4.1351

Cited by 11 publications

(2 citation statements)

References 19 publications

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“…The monoclonal antibody DBPA1 shows a high specificity for free S-(+)-ABA (Vernieri et al , 1989a; Walker–Simmons et al , 1991). Nevertheless, as it was the first time this antibody was used with soybean seed tissues, it was tested for the presence of competitive interference after high performance liquid chromatography (HPLC) fractionation of the crude aqueous extract (Vernieri et al , 1989b). In brief, an HPLC instrument equipped with a UV absorbance detector at 254 nm (Laboratory Data Control, Riviera Beach, Florida, USA) was used.…”

Section: Methodsmentioning

confidence: 99%

Endogenous abscisic acid and precocious germination of developing soybean seeds

Gosparini

Busilacchi

Vernieri³

et al. 2007

Seed Sci. Res.

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The germination of developing seeds is very uncommon and is generally associated with deficiencies in abscisic acid (ABA) synthesis or sensitivity. This paper examines the quantitative relationship between the inhibition of precocious germination and endogenous ABA in the embryonic axis (ABAa) of hydrated soybean [Glycine max (L.) Merr.] seeds, isolated after the completion of histodifferentiation and before the beginning of dehydration, as well as the magnitude and evolution of axis sensitivity to endogenous ABA during that period. Developing seeds harvested at 25, 30, 35, 40 and 45 d after anthesis (DAA) were subjected to incubation or washing to induce changes in ABA content. ABA content was measured by radioimmunoassay, using a monoclonal antibody against free ABA. Germinability was measured as the time to 50% germination (t 50 ). Washing and incubation induced eight-and twofold increases, respectively, in the rate of ABAa decline compared with the in planta ABAa decline. The threshold ABAa for inhibition of precocious germination (ABAc) increased slightly from 25 to 40 DAA [1.15 -1.66 mg ABA (g DW) 21 ]. This contrasted with the substantial decline in ABAa [10.90 -2.07 mg ABA (g DW) 21 ] during the same period, and indicated that sensitivity to endogenous ABA of hydrated seeds was initially high and diminished slowly during development. The relationship between (ABAa -ABAc) and t 50 was linear for immature seeds incubated before and after washing. Below the ABAc, there were no differences in the t 50 of 25 -45 DAA seeds. The ABAa contribution to the control of precocious soybean seed germination was evident, although other potentially interacting factors were also present.

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Section: Methodsmentioning

confidence: 99%

Endogenous abscisic acid and precocious germination of developing soybean seeds

Gosparini

Busilacchi

Vernieri³

et al. 2007

Seed Sci. Res.

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“…In fact, ABA content in the various tissues during seed development has been thoroughly measured in recent investigations carried out on Sechium edule (15) and Phaseolus coccineus (12).…”

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confidence: 99%

Level of Abscisic Acid in Integuments, Nucellus, Endosperm, and Embryo of Peach Seeds (Prunus persica L. cv Springcrest) during Development

Piaggesi¹,

Perata²,

Vitagliano³

et al. 1991

Plant Physiol.

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Free abscisic acid (ABA) in integuments, nucellus, endosperm, and embryo was determined throughout seed development of peach (Prunus persica L. cv Springcrest). Quantification of ABA was performed using combined high performance liquid chromatography-radioimmunoassay based on a monoclonal antibody raised against free (S)-ABA. In the integuments and endosperm, ABA concentration remained constant during the first 100 days after anthesis and rose in the following days when fresh weight was rapidly decreasing. In the nucellus, the ABA concentration variation pattern paralleled that of tissue growth. ABA concentration in the embryo increased constantly with the growth of the tissues to reach a maximum at the last growth stage. The role of ABA in peach seeds is discussed in relation to the development of the different seed tissues.Growth of the peach fruit occurs in three stages, as shown by Connors (4): rapid growth (stage I), delayed growth (stage II), and second rapid growth (stage III). The growth of the nucellus and integuments during stage I is parallel to the rapid growth ofthe pericarp, while embryo development is arrested. At the beginning of stage II (delayed development of the pericarp), the nucellus and integuments enter a period of delayed development, and the endosperm begins a rapid enlargement. During stage III, the nucellus degenerates and the embryo begins its development; depending on the variety, it reaches maximum size during this period.The endogenous systems that regulate this growth pattern are not known, but several scientists have described a regulating role played by hormones. A high content of gibberellins has been observed during peach seed development (6,17,18). Ethylene production also occurs at relatively high rates and, at the same time, 1 -aminocyclopropane-1 -carboxylic acid concentration peaks in the fruit (8, 10). Auxin level is also high during seed development, as shown by Miller et al. (1 1). Few data are available on ABA content in peach seeds (2) and particularly on ABA quantification in the various seed components during development.The availability of immunological methods for the quantification of ABA allows accurate analysis of ABA content over a great number of samples in much less time compared with the more traditional gas-chromatographic analysis. In fact, ABA content in the various tissues during seed development has been thoroughly measured in recent investigations carried out on Sechium edule (15) and Phaseolus coccineus (12).In this paper, we report on the quantitation of ABA in integuments, nucellus, endosperms, and embryos of Prunus persica cv Springcrest during seed development, with the aim of extending information on the hormonal relation between these seed components. MATERIALS AND METHODS Plant MaterialDeveloping fruits of peach (Prunus persica cv Springcrest), were harvested in the open field from May to June 1990. Care was taken to spread the sampling over the trees so that it would not drastically alter the crop load of any tree. Fruits were open...

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Changes in abscisic acid and its β-D-glucopyranosyl ester levels during tomato (Lycopersicon esculentum Mill.) seed development

et al. 1991

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The role of abscisic acid (ABA) in tomato (Lycopersicon esculentum Mill.) zygotic embryogenesis was analysed. ABA and ABA ß-D-glucopyranosyl ester (ABA-GE) changes were determined in seeds and fruit tissues - placenta and mesocarp - during seed development, which was defined with eight embryo stages: from globular (stage 1) to mature embryo (stage 8). In whole seeds, ABA changes paralleled fresh and dry weight pattern curves and could be characterized by a high increase during embryo growth followed by a decrease as the seed matured and dehydrated. Moreover this dehydration phase led, at stage 8, to a new ABA distribution within the seed, preferentially into integument and embryo. Fruit tissue analyses provided new information about the ABA origin in seeds. ABA-GE levels were also measured and the results suggested different ABA metabolism in seed and fruit tissues.

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Abscisic Acid Levels during Early Seed Development in Sechium edule Sw

Cited by 11 publications

References 19 publications

Endogenous abscisic acid and precocious germination of developing soybean seeds

Endogenous abscisic acid and precocious germination of developing soybean seeds

Level of Abscisic Acid in Integuments, Nucellus, Endosperm, and Embryo of Peach Seeds (Prunus persica L. cv Springcrest) during Development

Changes in abscisic acid and its β-D-glucopyranosyl ester levels during tomato (Lycopersicon esculentum Mill.) seed development

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