Absolute and Direct MicroRNA Quantification Using DNA–Gold Nanoparticle Probes

Degliangeli, Federica; Kshirsagar, Prakash; Brunetti, Virgilio; Pompa, Pier Paolo; Fiammengo, Roberto

doi:10.1021/ja412152x

Cited by 371 publications

(278 citation statements)

References 40 publications

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“…PCR is robust and reliable for DNA or RNA detection but requires sophisticated equipment and lengthy protocols. Instead, a one-pot nanoparticle biosensor assay could detect and quantify targets in complex mixtures, which has been demonstrated with superquenching AuNPs (81). Such assays could be kit-based, and users would generally be well skilled; therefore, these types of application are easy to envisage and would fit in easily alongside other research tools and methodologies.…”

Section: Moving Toward Applicationsmentioning

confidence: 99%

Colloidal nanoparticles as advanced biological sensors

Howes

Chandrawati

Stevens

2014

Science

905

686

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Colloidal nanoparticle biosensors have received intense scientific attention and offer promising applications in both research and medicine. We review the state of the art in nanoparticle development, surface chemistry, and biosensing mechanisms, discussing how a range of technologies are contributing toward commercial and clinical translation. Recent examples of success include the ultrasensitive detection of cancer biomarkers in human serum and in vivo sensing of methyl mercury. We identify five key materials challenges, including the development of robust mass-scale nanoparticle synthesis methods, and five broader challenges, including the use of simulations and bioinformatics-driven experimental approaches for predictive modeling of biosensor performance. The resultant generation of nanoparticle biosensors will form the basis of high-performance analytical assays, effective multiplexed intracellular sensors, and sophisticated in vivo probes.Evolution has given rise to organisms of staggering complexity. Our now extensive knowledge of biological systems pales in comparison to the remaining mysteries. Unraveling these requires tools that probe the molecular machinery of life and provide detailed feedback on complex networks of subtle interactions. Such tools are cornerstones of biomedical research and practice, and improvements in these lead directly to a better understanding of fundamental biology, monitoring of health, and diagnosis of disease. Colloidal nanoparticle biosensors are a class of biological probe that will not only yield improved biological sensing but also provide a step change in our ability to probe the biomolecular realm. Nanoparticles can act as high-performance sensors because nanomaterials exhibit unique and useful behaviors not present in their bulk form: for example, bright tunable fluorescence from semiconductor nanoparticles and localized surface plasmon resonance (LSPR) phenomena in metallic nanoparticles. These particles exhibit intense responses to incident light (or other stimuli), and the ability to modulate this response by interaction with target analytes makes them excellent biosensor signal transducers. Outputs can be quantitative or qualitative depending on the functionality of

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Section: Moving Toward Applicationsmentioning

confidence: 99%

Colloidal nanoparticles as advanced biological sensors

Howes

Chandrawati

Stevens

2014

Science

905

686

View full text Add to dashboard Cite

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“…In acute liver failure (ALF), miR-1224 may represent therapeutic target via inhibiting cell proliferation and promoting cell apoptotic [6]. In addition, using DNA-gold nanoparticle probes, miRNA could be better applied in clinical praxis as biomarkers [7]. One research group showed that miR-155-5p, miR-483-5p, and miR-451a may support the early diagnostic value of coronary plaque rupture [8].…”

Section: Introductionmentioning

confidence: 99%

miR-27b Suppresses Endothelial Cell Proliferation and Migration by Targeting Smad7 in Kawasaki Disease

Rong

Shen

et al. 2018

Cell Physiol Biochem

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Background/Aims: Increasing evidence indicates that microRNAs (miRNAs) play important roles in Kawasaki disease (KD). Our previous study demonstrated that hsa-miR-27b-3p (miR-27b) was up-regulated in KD serum. However, the specific role of miR-27b in KD remains unclear. We aimed to investigate that miR-27b could be a biomarker and therapeutic target for KD treatment. As well, the specific mechanism of miR-27b effecting endothelial cell functions was studied. Methods: The expression of miR-27b and Smad7 was measured by qRT-PCR. Gain-of-function strategy was used to observe the effect of miR-27b on human umbilical vein endothelial cells (HUVECs) proliferation and migration. Bioinformatics analyses were applied to predict miR-27b targets and then we verified Smad7 by a luciferase reporter assay. Western blot was performed to detect the protein expression of Smad7, PCNA, MMP9, MMP12 and TGF-β-related genes. Results: We confirmed that miR-27b was shown to be dramatically up-regulated in KD serum and KD serum-treated HUVECs and that elevated expression of miR-27b suppressed the proliferation and migration of HUVECs. Furthermore, our results verified that miR-27b mediated cell functions by affecting the TGF-β via targeting Smad7 in HUVECs. Conclusion: These results suggested that up-regulated miR-27b had a protective role in HUVECs proliferation and migration via targeting Smad7 and affecting TGF-β pathway. Therefore, miR-27b represented a potential biomarker for KD and may serve as a promising therapeutic target for KD treatment.

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“…Degliangeli et al [15] have proposed a strategy for miRNA quantification based on DNA-AuNPs probes. As shown in Fig.…”

Section: Noble Metal Nanoparticlesmentioning

confidence: 99%

miRNA Optical Detection

Zhang

Dong

Tian

2015

SpringerBriefs in Molecular Science

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miRNA is a kind of attractive candidates, as biomarkers, for early cancer diagnosis. Therefore, simple and novel strategies for miRNA detection with high sensitivity and selectivity have great significance not only for the studies of its biological functions but also for clinical cancer diagnosis. Various reliable optical miRNA detection methods have become a class of attractive and paramount for miRNA expression analysis, due to its high sensitivity, wide dynamic range, and multiplexing capabilities. The progress of nanotechnology and nanoscience allows nanomaterial-based signal amplification to develop highly sensitive and selective biosensors for in situ or online detection of miRNA. Molecular biology-based strategies for miRNA detection by amplifying the target or probe with high detection sensitivity are applied in identifying and detecting nucleic acids analysis.Keywords Optical detection · Signal amplification · Noble metal nanoparticles · Transition metal dichalcogenides · Quantum dots · Canbon nanomaterials · Target or probe amplification Nanomaterials-Based miRNA Optical DetectionThe development of nanotechnology and nanoscience renders nanomaterial-based signal amplification great promise in developing highly sensitive and selective biosensors for in situ or online detection of miRNA. Nanomaterials-based biosensors exhibit the significant advantages with high sensitivity, wide dynamic range, and multiplexing capabilities compared to bulk materials-biosensors. Nanoparticles (NPs)-based biosensors show the significant advantages as follows: (i) The NPs allow design of low cost and minimized equipment in point-of-care diagnostics due to the small sizes breaking through the limitation of structure miniaturization. (ii) The direct contact between NPs and the environment lead to accelerated

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Absolute and Direct MicroRNA Quantification Using DNA–Gold Nanoparticle Probes

Cited by 371 publications

References 40 publications

Colloidal nanoparticles as advanced biological sensors

Colloidal nanoparticles as advanced biological sensors

miR-27b Suppresses Endothelial Cell Proliferation and Migration by Targeting Smad7 in Kawasaki Disease

miRNA Optical Detection

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