Absolute quantification of the microbiota spatial distribution in the murine large intestine

Abstract

<p>The species diversity, abundance, and location of intestinal bacteria bear major consequences on host intestinal functions, affecting the balance between health and disease. Recent studies addressed the relative abundance of bacteria in feces and on the gut mucosal surface. However, few of these publications reported the absolute number of bacteria in different compartments of the gut, especially those associated with the intestinal epithelial surface, compared to the luminal content reflected by feces. In this study, the number of bacteria present in the lumen or associated with the mucosal surface of mice gut samples was calculated using the bacterial ��internal standard method�� during sequencing. Three spiking bacteria were used for mutual corroboration. The microbiota spatial distribution in the cecum, proximal and distal colon were studied in the lumen content and gut wall fixed niches. This study also provides an example to demonstrate that absolute and relative quantification may yield different conclusions. This study��s method may eliminate such misjudgments and provide insight into the distribution and quantity of gut microbiome.</p>

New antibiotic against multi-drug resistant bacteria

“…Indepth genetic analyses, particularly pertaining to the LPS transport and biosynthesis machinery under the duress of antibiotic stress, are imperative. Pahil, K. S. et al 3 complements Zampaloni, C. et al 1 findings by unveiling a novel antibiotic mechanism targeting the LPS transport pathway in Acinetobacter. This research, showcasing a unique approach by trapping LPS within its transporter, underscores the significance of inhibiting LPS transport for bacterial membrane integrity.…”

Bacterial load in meconium

“…Wen-Yu Jin 1,2 Jing Peng 3 Jinping Dai 3 Rongkang Tang 1 Jia-Xin Guo Email: wangjielin369@126.com Wen-Yu Jin and Jing Peng contributed equally to this study.…”

“…The intestinal microbiota is host to an exceedingly large number of bacteria, estimated to be about 10 11 CFU/g in the adult colon [ 1 ]. With the development of metagenomics, metabolomics, and other omics technologies [ 2 ], the composition and function of gut microbes have been further analyzed and recognized [ 3 ]. Existing studies have shown that metabolites of the gut microbiota may be associated with the development of a variety of diseases [ 4 ].…”