Abstract 5435: A novel HDAC inhibitor, CKD-581, demonstrates a potent in vivo efficacy in various human tumor xenograft models

Abstract

Histone deacetylases (HDAC) inhibition is now well established as a new approach for solid and hematological tumor therapy. Studies with different HDAC inhibitors showed broad activity toward cancer cells, i.e. activation of proapoptotic pathway and inhibition of antiapoptotic pathway, induction of cell differentiation, antiangiogenic activity and synergism with other cancer therapeutics. Several HDAC inhibitors have demonstrated therapeutic benefits and are under clinical investigations as mono- or combi-therapy in various cancer cell lines such as cutaneous T-cell lymphoma (CTCL) with SAHA launched in 2006. Highly water-soluble CKD-581 is a potent and novel pan HDAC inhibitor developed in our institute. In this study, we analyzed first the in vitro growth inhibitory effect of CKD-581 on various cancer cells by MTT assay and inhibition of HDAC enzymes as well as its antitumor efficacy in human colon, prostate, and lung xenograft models. In addition, western blotting analysis for acH3, acH4 and p21 (WAF-1/CIP-1) and fluorescence-activated cell sorting analysis were performed to verify the associated molecular mechanisms involved in CKD-581 mediated cell death and cell cycle progression. CKD-581 showed strong cytotoxicity against several cancer cell lines including HCT-116, PC-3, A549 and H460 with IC50 values ranging from 10 nM to 100 nM. Its cytotoxicity was closely related with potent inhibitory activity against human HDAC1 and HDAC6 enzymes at single nMs. In various cancer cell lines, CKD-581 induced acetylation of histone and expression of tumor suppressor proteins involved in apoptosis pathway. CKD-581 significantly inhibited the in vivo growth of human tumor xenografts (HCT-116, PC-3, A549 and H460) in nude mice in a dose-dependent manner. Treatment of CKD-581 (60, 80, 100 mg/kg, b.i.wk, i.p.) caused 50-70% reduction in the mean tumor volume compared with controls. It is of note to observe that CKD-581 was highly efficacious when orally administered to nude mice bearing HCT 116. To correlate the pharmacodynamics with pharmacokinetics of CKD-581 in nude mice (HCT-116), we analyzed the kinetics of histone acetylation in tumors and plasma drug exposure at the end of 3 weeks of treatment (80 mg/kg, b.i.wk, i.p.), where Tmax of CKD-581 in tumor and in plasma were 4 h and 0.25 h after dosing, respectively and AUClast in tumor was 2.6 times higher than that of in plasma. CKD-581 is a potent HDAC inhibitor and showed a strong cytotoxicity against many cancer cell lines, demonstrated through the various in vitro and in vivo studies. Moreover, significant antitumor efficacy was shown in nude mice bearing several human tumors. Its potency together with excellent pharmacokinetic properties warrants further clinical investigations of CKD-581. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 5435.

CKD-5, a novel pan-histone deacetylase inhibitor, synergistically enhances the efficacy of sorafenib for hepatocellular carcinoma

“…In preclinical in vitro and in vivo studies, CKD-5 has demonstrated strong anti-tumor effects against multiple myeloma and cutaneous T-cell lymphoma [ 16 , 17 ]. CKD-5 has also shown considerable cytotoxicity in multiple solid tumor cell lines and xenograft mouse models of colon, prostate, and lung cancer [ 18 ]. A phase I study of CKD-5 recently reported promising results with minimal side effects and modest anti-tumor efficacy in patients with lymphoma or multiple myeloma refractory to standard therapy [ 19 ].…”