The morbidity and mortality of lung cancer rank second and first respectively in malignant solid tumors worldwide. As we all know, surgical resection is the cornerstone of comprehensive treatment of non-small-cell lung cancer (NSCLC). The current National Comprehensive Cancer Network (NCCN) guidelines for NSCLC suggest that for medically operable disease, resection is the preferred local treatment modality, anatomic pulmonary resection is preferred for the majority of patients with NSCLC, and video-assisted thoracoscopic surgery (VATS) or minimally invasive surgery should be strongly considered for patients with no anatomic or surgical contraindications. With many advantages, uniportal VATS (u-VATS) has been widely accepted and used. Therefore, in this article, we attempted to review the essentials of lobectomy under u-VATS for NSCLC.