AC Electroosmosis Effect on Microfluidic Heterogeneous Immunoassay Efficiency

Selmi, Marwa; Belmabrouk, Hafedh

doi:10.3390/mi11040342

Cited by 15 publications

(10 citation statements)

References 37 publications

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“…In this experiment, the phenomenon of induced charge electroosmosis in a rotating electric field (ROT-ICEO) was the core innovation to improve the reaction efficiency [95]. Similarly, in 2020, Selmi and Belmabrouk applied the AC electroosmosis (ACEO) to produce swirling structures in the fluid through which the transportation efficiency of the antigen to the immobilized antibodies was greatly enhanced [96].…”

Section: Electrophoresis Microfluidic System In Immunoassaysmentioning

confidence: 99%

Application of Microfluidics in Immunoassay: Recent Advancements

Shi

Peng

Yang

et al. 2021

Journal of Healthcare Engineering

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In recent years, point-of-care testing has played an important role in immunoassay, biochemical analysis, and molecular diagnosis, especially in low-resource settings. Among various point-of-care-testing platforms, microfluidic chips have many outstanding advantages. Microfluidic chip applies the technology of miniaturizing conventional laboratory which enables the whole biochemical process including reagent loading, reaction, separation, and detection on the microchip. As a result, microfluidic platform has become a hotspot of research in the fields of food safety, health care, and environmental monitoring in the past few decades. Here, the state-of-the-art application of microfluidics in immunoassay in the past decade will be reviewed. According to different driving forces of fluid, microfluidic platform is divided into two parts: passive manipulation and active manipulation. In passive manipulation, we focus on the capillary-driven microfluidics, while in active manipulation, we introduce pressure microfluidics, centrifugal microfluidics, electric microfluidics, optofluidics, magnetic microfluidics, and digital microfluidics. Additionally, within the introduction of each platform, innovation of the methods used and their corresponding performance improvement will be discussed. Ultimately, the shortcomings of different platforms and approaches for improvement will be proposed.

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Section: Electrophoresis Microfluidic System In Immunoassaysmentioning

confidence: 99%

Application of Microfluidics in Immunoassay: Recent Advancements

Shi

Peng

Yang

et al. 2021

Journal of Healthcare Engineering

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“…ACEO is an electrokinetic phenomenon induced by the application of AC voltage to microelectrodes, causing circulating flow above the electrode surface that mixes the analyte solution near the surface and increases the chance of analyte molecules binding to the ligands on the surface [ 12 , 13 , 14 , 15 , 16 ]. The use of electrokinetic phenomena has been reported in the literature [ 17 , 18 ]; however, it requires a complex optical system for imaging sensor chips designed for SPR imaging [ 19 , 20 ], and the improvement of protein binding has not been proven with a sensing setup without imaging function.…”

Section: Introductionmentioning

confidence: 99%

AC-Electroosmosis-Assisted Surface Plasmon Resonance Sensing for Enhancing Protein Signals with a Simple Kretschmann Configuration

Terao

Kondo

2022

Sensors

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A surface plasmon resonance (SPR) sensor chip fabricated with a comb-shaped microelectrode array to supply alternating current (AC) voltage is reported. The chip induces circulating flow near the surface (i.e., AC electroosmosis). The circulating flow provides a mixing effect, which enhances the binding of the analyte molecules. We evaluated the SPR characteristics of the chip and demonstrated an improvement in protein binding to the chip surface. SPR sensor chips with comb-shaped microelectrodes were fabricated using standard UV lithography. Sensing experiments were conducted using a standard Kretschmann-type SPR measurement system. To demonstrate the mixing effect of AC electroosmosis, we evaluated the binding of immunoglobulin G molecules onto the sensor surface where anti-immunoglobulin G antibodies were covalently immobilized. The result indicates that the amount of binding increases by a factor of 1.7 above that achieved by using a conventional chip, suggesting enhancement of the protein signal.

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“…To enhance the biosensor binding reaction rate, many physical mechanisms have been applied to obtain agitation flow models in microfluidic channels such as hydrodynamic pressure (Chiu et al 2013), AC electrokinetics (ACEK) (Yang et al 2017;Selmi and Belmabrouk 2020), magnetic effect (Saad et al 2021) and optical forces (Lee et al 2000). The ACEK phenomenon mainly includes the dielectrophoresis (DEP), AC electroosmosis (ACEO), and AC electrothermal effects (ACET).…”

Section: Introductionmentioning

confidence: 99%

Enhancement of COVID-19 detection time by means of electrothermal force

Kaziz

Saad

Bouzid

et al. 2021

Microfluid Nanofluid

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The rapid spread and quick transmission of the new ongoing pandemic coronavirus disease 2019 has urged the scientific community to looking for strong technology to understand its pathogenicity, transmission, and infectivity, which helps in the development of effective vaccines and therapies. Furthermore, there was a great effort to improve the performance of biosensors so that they can detect the pathogenic virus quickly, in reliable and precise way. In this context, we propose a numerical simulation to highlight the important role of the design parameters that can significantly improve the performance of the biosensor, in particular the sensitivity as well as the detection limit. Applied alternating current electrothermal (ACET) force can generate swirling patterns in the fluid within the microfluidic channel, which improve the transport of target molecule toward the reaction surface and, thus, enhance the response time of the biosensor. In this work, the ACET effect on the SARS-CoV-2 S protein binding reaction kinetics and on the detection time of the biosensor was analyzed. Appropriate choice of electrodes location on the walls of the microchannel and suitable values of the dissociation and association rates of the binding reaction, while maintaining the same affinity, with and without ACET effect, are also, discussed to enhance the total performance of the biosensor and reduce its response time. The two-dimensional equations system is solved by the finite element approach. The best performance of the biosensor is obtained in the case where the response time decreased by 61% with AC applying voltage.

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AC Electroosmosis Effect on Microfluidic Heterogeneous Immunoassay Efficiency

Cited by 15 publications

References 37 publications

Application of Microfluidics in Immunoassay: Recent Advancements

Application of Microfluidics in Immunoassay: Recent Advancements

AC-Electroosmosis-Assisted Surface Plasmon Resonance Sensing for Enhancing Protein Signals with a Simple Kretschmann Configuration

Enhancement of COVID-19 detection time by means of electrothermal force

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