Acacetin inhibits myocardial mitochondrial dysfunction by activating PI3K/AKT in SHR rats fed with fructose

Yuan, Peipei; Zhang, Qi; Fu, Yang; Hou, Ying; Gao, Liyuan; Wei, Yaxin; Feng, Weisheng; Zheng, Xiaoke

doi:10.1007/s11418-022-01666-7

Cited by 6 publications

(5 citation statements)

References 28 publications

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“…Our previous studies confirmed that acacetin improved myocardial mitochondrial dysfunction by regulating the PI3 K/AKT signaling pathway-mediated oxidative stress, mitochondrial fission and fusion, and apoptosis of the mitochondrial pathway in fructose-induced SHR rats. 14 As shown in this study, AC can regulate RAAS system and endothelial cell correlated factors, activate the key protein PI3Kp85 subunit of the insulin signaling pathway, and improve SHR & IR cardiac endothelial mesenchymal conversion and myocardial fibrosis in rats. As an antagonist of PI3 K, wortmannin did not exert the expected antagonist effect on acacetin.…”

Section: Discussionmentioning

confidence: 55%

“…It has been reported that acacetin (AC) has the effect of lowering blood pressure, improving myocardial damage caused by ischemia-reperfusion, 14,15 and improving lipid metabolism disorders. 16 At the same time, the previous research of our group has established the fructose-induced SHR model accompanied by metabolic syndrome successfully, and also found that acacetin improved myocardial mitochondrial dysfunction through regulating oxidative stress, mitochondrial fission and fusion, and mitochondrial pathway apoptosis mediated by PI3 K/ AKT signaling pathway in hypertensive rats with IR.…”

Section: Introductionmentioning

confidence: 99%

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Acacetin Combined with Wortmannin Activated Insulin Signaling Pathway by Inhibiting the Induction Effect of PI3Kp110 on IRS-1 Serine Phosphorylation to Improve Myocardial Fibrosis in Fructose-Induced SHR Rats

Zhao,

Yuan,

Zhang

et al. 2023

Natural Product Communications

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Objective To explore the intervention mechanism of acacetin combined with wortmannin on myocardial fibrosis in fructose-induced SHR rats. Methods Using fructose-induced SHR rats combined with HUVEC cell induced by high-sugar DMEM, the intervention mechanism of acacetin combined with wortmannin on myocardial fibrosis in rats was explored. Results In fructose-induced SHR rats, the intervention results of acacetin on myocardial fibrosis showed that acacetin significantly downregulated serum REN, Ang II, ET-1 levels, and upregulated serum PGE2 and NO levels ( P < 0.05 or P < 0.01). The combination of wortmannin showed further enhanced effect on the level of ET-1 and PGE2 ( P < 0.05). Acacetin and wortmannin significantly increased the expression of CD31 protein and CD31, VE-cadherin mRNA levels in the heart, decreased the expression of CVF, α-SMA protein and CVF, α-SMA, Vimentin and Fibronectin mRNA levels, and enhanced the improvement effect after the combination of acacetin and wortmannin ( P < 0.05 or P < 0.01). Both acacetin and wortmannin significantly activated the level of PI3Kp85 in myocardium ( P < 0.05 or P < 0.01), and wortmannin significantly inhibited PI3Kp110 ( P < 0.01). While, acacetin combined with wortmannin, the activation effect on PI3Kp85 was enhanced, and the inhibitory effect on PI3Kp110 was weakened ( P < 0.01). In HUVEC endothelial mesenchymal transformation model, wortmannin significantly inhibited the phosphorylation of IRS-1 serine ( P < 0.01); After PI3Kp85 silencing, both acacetin and wortmannin inhibited the activation of PI3 K downstream protein AKT phosphorylation ( P < 0.01). Conclusion Acacetin synergistic with PI3 K inhibitor wortmannin inhibits the induction effect of PI3Kp110 on IRS-1 serine phosphorylation, indirectly activates insulin signaling pathway, modulates vasoactive factors, alleviates cardiac endothelial mesenchymal transformation in fructose-induced SHR rats, and improves myocardial fibrosis.

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Section: Discussionmentioning

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Acacetin Combined with Wortmannin Activated Insulin Signaling Pathway by Inhibiting the Induction Effect of PI3Kp110 on IRS-1 Serine Phosphorylation to Improve Myocardial Fibrosis in Fructose-Induced SHR Rats

Zhao,

Yuan,

Zhang

et al. 2023

Natural Product Communications

Self Cite

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“…In this study, KEGG enrichment analysis indicated that 832 differentially expressed mRNAs were mainly involved in metabolic, NOD-like receptor, Wnt, PPAR, and p53 signaling pathways and in glycerophospholipid metabolism. The target genes of the differentially expressed miR-NAs were mainly involved in endocytosis, the PI3K-Akt signaling pathway, nucleocytoplasmic transport, MAPK signaling pathway, and viral carcinogenesis pathways, among these, the NOD-like receptor, Wnt, PPAR, PI3K-Akt, and MAPK signaling pathways were closely related to mitochondrial function [35][36][37]. For example, NODlike receptor family member X1 (NLRX1) localized to the mitochondrial outer membrane.…”

Section: Discussionmentioning

confidence: 99%

Integrated analysis of microRNA and messenger RNA expression profiles reveals functional microRNA in infectious bovine rhinotracheitis virus-induced mitochondrial damage in Madin-Darby bovine kidney cells

Ma,

Guo,

et al. 2024

BMC Genomics

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Background Studies have confirmed that Infectious bovine rhinotracheitis virus (IBRV) infection induces mitochondrial damage. MicroRNAs (miRNAs) are a class of noncoding RNA molecules, which are involved in various biological processes and pathological changes associated with mitochondrial damage. It is currently unclear whether miRNAs participate in IBRV-induced mitochondrial damage in Madin-Darby bovine kidney (MDBK) cells. Results In the present study, we used high-throughput sequencing technology, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis to screen for mitochondria-related miRNAs and messenger RNAs (mRNAs). In total, 279 differentially expressed miRNAs and 832 differentially expressed mRNAs were identified in 6 hours (IBRV1) versus 24 hours (IBRV2) after IBRV infection in MDBK cells. GO and KEGG enrichment analysis revealed that 42 differentially expressed mRNAs and 348 target genes of differentially expressed miRNAs were correlated with mitochondrial damage, and the miRNA-mitochondria-related target genes regulatory network was constructed to elucidate their potential regulatory relationships. Among the 10 differentially expressed miRNAs, 8 showed expression patterns consistent with the high-throughput sequencing results. Functional validation results showed that overexpression of miR-10a and miR-182 aggravated mitochondrial damage, while inhibition of miR-10a and miR-182 alleviated mitochondrial damage. Conclusions This study not only revealed the expression changes of miRNAs and mRNAs in IBRV-infected MDBK cells, but also revealed possible biological regulatory relationship between them. MiR-10a and miR-182 may have the potential to be developed as biomarkers for the diagnosis and treatment of IBRV. Together, Together, these data and analyses provide additional insights into the roles of miRNA and mRNA in IBRV-induced mitochondria damage

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“…In the present study, The KEGG enrichment analysis indicated that 832 differentially expressed mRNAs were involved mainly in metabolic, NOD-like receptor, Wnt, PPAR, and p53 signaling pathways and in glycerophospholipid metabolism. The target genes of the differentially expressed miRNAs were involved mainly in endocytosis, the PI3K-Akt signaling pathway, nucleocytoplasmic transport, MAPK signaling pathway, and viral carcinogenesis pathways, among these, the NOD-like receptor, Wnt, PPAR, PI3K-Akt, and MAPK signaling pathways were closely related to mitochondrial function [35][36][37]. For example, NODlike receptor family member X1 (NLRX1) localized to the mitochondrial outer membrane.…”

Section: Discussionmentioning

confidence: 99%

Integrated analysis of microRNA and messenger RNA expression profiles reveals functional microRNA in infectious bovine rhinotracheitis virus-induced mitochondrial damage in Madin-Darby bovine kidney cells

Guo

et al. 2023

Preprint

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Background Studies have confirmed that Infectious bovine rhinotracheitis virus (IBRV) infection induces mitochondrial damage. MicroRNAs (miRNAs) are a class of noncoding RNA molecules, which are involved in various biological processes and pathological changes associated with mitochondrial damage. It is not known whether miRNAs participate in IBRV-induced mitochondrial damage in Madin-Darby bovine kidney (MDBK) cells. Results In the present study, we used high-throughput sequencing technology, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis to screen mitochondria-related miRNAs and messenger RNAs (mRNAs). In total, 279 differentially expressed miRNAs and 832 differentially expressed mRNAs were identified in 6 hours (IBRV1) versus 24 hours (IBRV2) after IBRV infection in MDBK cells. GO and KEGG enrichment analysis revealed that 42 differentially expressed mRNAs and 348 target genes of differentially expressed miRNAs were correlated with mitochondrial damage, and the miRNA-mitochondria-related target genes regulatory network was constructed to elucidate their underlying regulatory relationships. The expression patterns of eight of ten differentially expressed miRNAs were consistent with the high-throughput sequencing results, Functional validation results showed that the overexpression of miR-10a and miR-182 aggravated mitochondrial damage and that the inhibition of miR-10a and miR-182 alleviated mitochondrial damage. Conclusions This study revealed not only the expression changes of miRNAs and mRNAs in IBRV-infected MDBK cells but also the possible biological regulatory relationship between them. MiR-10a and miR-182 have the potential to be developed as biomarkers for IBRV diagnosis and treatment. Together, these data and analyses provide additional insight into the role of miRNAs and mRNAs in IBRV-induced mitochondria damage.

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Acacetin inhibits myocardial mitochondrial dysfunction by activating PI3K/AKT in SHR rats fed with fructose

Cited by 6 publications

References 28 publications

Acacetin Combined with Wortmannin Activated Insulin Signaling Pathway by Inhibiting the Induction Effect of PI3Kp110 on IRS-1 Serine Phosphorylation to Improve Myocardial Fibrosis in Fructose-Induced SHR Rats

Acacetin Combined with Wortmannin Activated Insulin Signaling Pathway by Inhibiting the Induction Effect of PI3Kp110 on IRS-1 Serine Phosphorylation to Improve Myocardial Fibrosis in Fructose-Induced SHR Rats

Integrated analysis of microRNA and messenger RNA expression profiles reveals functional microRNA in infectious bovine rhinotracheitis virus-induced mitochondrial damage in Madin-Darby bovine kidney cells

Integrated analysis of microRNA and messenger RNA expression profiles reveals functional microRNA in infectious bovine rhinotracheitis virus-induced mitochondrial damage in Madin-Darby bovine kidney cells

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