Accelerated antibiotic susceptibility testing of pseudomonas aeruginosa by monitoring extracellular electron transfer on a 3-D paper-based cell culture platform

Rafiee, Zahra; Rezaie, Maryam; Choi, Seokheun

doi:10.1016/j.bios.2022.114604

Cited by 9 publications

(12 citation statements)

References 38 publications

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“…This accumulated output power through time is defined as energy, “ E ”, as described in the following,where P is the output power measured from the device at time t , and T is the total accumulated time in seconds. 19,20 With the accumulation of electrical outputs through the bacterial EET, the AST device even with low bacterial concentrations demonstrates distinguishable antibiotic effectiveness. The limit of detection (LOD) of our sensor goes down to a lower level of 0.1 OD 600 which is equivalent to a 0.5 McFarland standard.…”

Section: Resultsmentioning

confidence: 99%

“…Based on our previous report, even the small bacterial concentration of 0.1 OD 600 (corresponding to 10 8 CFU ml −1 and the 0.5 McFarland turbidity standard) had readily and rapidly formed densely packed bacterial colonies in the 3-D porous cellulose structure while the antibiotic substances were effectively delivered to the individual cells in the biofilm. 19 The energy curves were obtained by subtracting the bacteria-free aseptic control from the bacterial sample to avoid abiotic noises and environmental interferences. All experiments were repeated at least three times and their standard deviations were plotted as a shaded area.…”

Section: Resultsmentioning

confidence: 99%

“…23 In particular, the microbial fuel cell has been proposed as the next viable AST technology as the bacterial metabolism and their EET can be significantly affected by their susceptibility and resistivity to antibiotics. 19,20,23 Moreover, many pathogens are electrogenic, and more and more pathogens have shown weak electrogenic capabilities. 24 Such pathogens include Enterococcus faecalis, Klebsiella variicola, Listeria monocytogenes, and Pseudomonas aeruginosa.…”

Section: Bacteria Device Design and Operationmentioning

confidence: 99%

“…24 Such pathogens include Enterococcus faecalis, Klebsiella variicola, Listeria monocytogenes, and Pseudomonas aeruginosa. Although bacterial EET-based ASTs have been demonstrated electrochemically with a potentiostat 25 or electrically in a microbial fuel cell, 19,20 quantifying the signal of those weak electrogenes and establishing their medical relevance as a reliable AST technique is by far the most challenging part to approach. Especially, advances in this domain require establishing in vitro biofilm models that can represent 3-D clinical infections.…”

Section: Bacteria Device Design and Operationmentioning

confidence: 99%

“…Very recently, we successfully demonstrated that the EET profiles from the bacterial biofilm in the paper-based culturing platform had been sensitively responding to the antibiotics. 19,20 However, the reports failed to describe how the EET outputs can qualify as a real AST method that identifies antibiotic-susceptible and -resistant bacteria and how the outputs can innovatively identify the antibiotic mechanisms of action. In this work, we show how practically the bacterial EET on the paper-based bioelectrochemical system can be used to monitor antibiotic efficacy, quantify the MIC values, and provide mechanistic insights into antibiotic action.…”

Section: Introductionmentioning

confidence: 99%

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All-electrical antibiotic susceptibility and resistance profiling of electrogenic Pseudomonas aeruginosa

Rafiee

Choi

2023

Analyst

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Bacteria Device Design and Operationmentioning

confidence: 99%

Section: Bacteria Device Design and Operationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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All-electrical antibiotic susceptibility and resistance profiling of electrogenic Pseudomonas aeruginosa

Rafiee

Choi

2023

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Cellulosic Nanofibers Utilizing a Silicone Elastomeric Core to Form Stretchable Paper

Dorsainvil,

Brown,

Rafiee

et al. 2023

Adv Materials Inter

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Paper, an inexpensive material with natural biocompatibility, non‐toxicity, and biodegradability, allows for affordable and cost‐effective substrates for unconventional advanced electronics, often called papertronics. On the other hand, polymeric elastomers have shown to be an excellent success for substrates of soft bioelectronics, providing stretchability in skin wearable technology for continuous sensing applications. Although both materials hold their unique advantageous characteristics, merging both material properties into a single electronic substrate reimagines paper‐based bioelectronics for wearable and patchable applications in biosensing, energy generation and storage, soft actuators, and more. Here, a breathable, light‐weighted, biocompatible engineered stretchable paper is reported via coaxial nonwoven microfibers for unconventional bioelectronic substrates. The stretchable papers allow intimate bioconformability without adhesive through coaxial electrospinning of a cellulose acetate polymer (sheath) and a silicone elastomer (core). The fabricated cellulose‐silicone fibers exhibit a greater percent strain than commercially available paper while retaining hydrophilicity, biocompatibility, combustibility, disposable, and other natural characteristics of paper. Moreover, the nonwoven stretchable cellulose‐silicone fibrous mat can adapt conventional printing and fabrication process for paper‐based electronics, an essential aspect of advanced bioelectronic manufacturing.

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Sensor system for analysis of biofilm sensitivity to ampicillin

Guliy,

Evstigneeva,

Shirokov

et al. 2024

Appl Microbiol Biotechnol

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The resistance of biofilms to antibiotics is a key factor that makes bacterial infections unsusceptible to antimicrobial therapy. The results of classical tests of cell sensitivity to antibiotics cannot be used to predict therapeutic success in infections associated with biofilm formation. We describe a simple and rapid method for the real-time evaluation of bacterial biofilm sensitivity to antibiotics, with Pseudomonas putida and ampicillin as examples. The method uses an electric biosensor to detect the difference between changes in the biofilm electric polarizability, thereby evaluating antibiotic sensitivity. The electric signals showed that P. putida biofilms were susceptible to ampicillin and that at high antibiotic concentrations, the biofilms differed markedly in their susceptibility (dose-dependent effect). The sensor also detected differences between biofilms before and after ampicillin treatment. The electric-signal changes enabled us to describe the physical picture of the processes occurring in bacterial biofilms in the presence of ampicillin. The approach used in this study is promising for evaluating the activity of various compounds against biofilms, because it permits a conclusion about the antibiotic sensitivity of biofilm bacteria to be made in real time and in a short period (analysis time, not longer than 20 min). An added strong point is that analysis can be done directly in liquid, without preliminary sample preparation. Key points • Sensor system to analyze biofilm antimicrobial susceptibility is described. • The signal change depended on the ampicillin concentration (dose-dependent effect). • The sensor allows real-time determination of the antibiofilm effect of ampicillin.

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Accelerated antibiotic susceptibility testing of pseudomonas aeruginosa by monitoring extracellular electron transfer on a 3-D paper-based cell culture platform

Cited by 9 publications

References 38 publications

All-electrical antibiotic susceptibility and resistance profiling of electrogenic Pseudomonas aeruginosa

All-electrical antibiotic susceptibility and resistance profiling of electrogenic Pseudomonas aeruginosa

Cellulosic Nanofibers Utilizing a Silicone Elastomeric Core to Form Stretchable Paper

Sensor system for analysis of biofilm sensitivity to ampicillin

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