Accelerated start-up, long-term performance and microbial community shifts within a novel upflow porous-plated anaerobic reactor treating nitrogen-rich wastewater <i>via</i> ANAMMOX process

Zhang, Dachao; Shi, Xu; Antwi, Philip; Xiao, Longwen; Luo, Wuhui; Liu, Zuwen; Li, Jianzheng; Su, Hao; Lai, Cheng; Ayivi, Frederick

doi:10.1039/c9ra04225c

Cited by 19 publications

(3 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Both approaches showed concordance with each other, suggesting that the genome-based taxonomic assignments were sufficient to cover the MAG lineages within the PNA system. Microorganisms belonging to the phyla Bacteroidota , Chloroflexota , Proteobacteria , and Planctomycetota are always identified in anammox-related ecosystems [ 13 – 15 , 68 ]. The newly recovered (near-) complete MAGs will contribute to a further characterization of the bacterial repertoire of anammox-associated systems, as well as microbial ecology studies integrated with other technologies.…”

Section: Resultsmentioning

confidence: 99%

High-quality bacterial genomes of a partial-nitritation/anammox system by an iterative hybrid assembly method

et al. 2020

View full text Add to dashboard Cite

Background Genome-centric approaches are widely used to investigate microbial compositions, dynamics, ecology, and interactions within various environmental systems. Hundreds or even thousands of genomes could be retrieved in a single study contributed by the cost-effective short-read sequencing and developed assembly/binning pipelines. However, conventional binning methods usually yield highly fragmented draft genomes that limit our ability to comprehensively understand these microbial communities. Thus, to leverage advantage of both the long and short reads to retrieve more complete genomes from environmental samples is a must-do task to move this direction forward. Results Here, we used an iterative hybrid assembly (IHA) approach to reconstruct 49 metagenome-assembled genomes (MAGs), including 27 high-quality (HQ) and high-contiguity (HC) genomes with contig number ≤ 5, eight of which were circular finished genomes from a partial-nitritation anammox (PNA) reactor. These 49 recovered MAGs (43 MAGs encoding full-length rRNA, average N50 of 2.2 Mbp), represented the majority (92.3%) of the bacterial community. Moreover, the workflow retrieved HQ and HC MAGs even with an extremely low coverage (relative abundance < 0.1%). Among them, 34 MAGs could not be assigned to the genus level, indicating the novelty of the genomes retrieved using the IHA method proposed in this study. Comparative analysis of HQ MAG pairs reconstructed using two methods, i.e., hybrid and short reads only, revealed that identical genes in the MAG pairs represented 87.5% and 95.5% of the total gene inventory of hybrid and short reads only assembled MAGs, respectively. In addition, the first finished anammox genome of the genus Ca. Brocadia reconstructed revealed that there were two identical hydrazine synthase (hzs) genes, providing the exact gene copy number of this crucial phylomarker of anammox at the genome level. Conclusions Our results showcased the high-quality and high-contiguity genome retrieval performance and demonstrated the feasibility of complete genome reconstruction using the IHA workflow from the enrichment system. These (near-) complete genomes provided a high resolution of the microbial community, which might help to understand the bacterial repertoire of anammox-associated systems. Combined with other validation experiments, the workflow can enable a detailed view of the anammox or other similar enrichment systems.

show abstract

Section: Resultsmentioning

confidence: 99%

High-quality bacterial genomes of a partial-nitritation/anammox system by an iterative hybrid assembly method

et al. 2020

View full text Add to dashboard Cite

show abstract

“…A major difficulty for such enrichment is the denitrification in lake water–sediment interface always inhibits the start-up of anammox. Thus, establishing a quick start-up of anammox enrichment with lake sediments is of great importance for studying N removal in eutrophic lake ecosystems [ 48 , 49 ]. This study successfully started up the anammox enrichment with eutrophic lake sediments, and the maximum removal of NH 4 + and NO 2 − reached to 85% and 95%, respectively.…”

Section: Discussionmentioning

confidence: 99%

Ecological interactions and the underlying mechanism of anammox and denitrification across the anammox enrichment with eutrophic lake sediments

et al. 2023

View full text Add to dashboard Cite

Background Increasing attention has recently been devoted to the anaerobic ammonium oxidation (anammox) in eutrophic lakes due to its potential key functions in nitrogen (N) removal for eutrophication control. However, successful enrichment of anammox bacteria from lake sediments is still challenging, partly due to the ecological interactions between anammox and denitrifying bacteria across such enrichment with lake sediments remain unclear. Results This study thus designed to fill such knowledge gaps using bioreactors to enrich anammox bacteria with eutrophic lake sediments for more than 365 days. We continuously monitored the influent and effluent water, measured the anammox and denitrification efficiencies, quantified the anammox and denitrifying bacteria, as well as the related N cycling genes. We found that the maximum removal efficiencies of NH4+ and NO2− reached up to 85.92% and 95.34%, respectively. Accordingly, the diversity of anammox and denitrifying bacteria decreased significantly across the enrichment, and the relative dominant anammox (e.g., Candidatus Jettenia) and denitrifying bacteria (e.g., Thauera, Afipia) shifted considerably. The ecological cooperation between anammox and denitrifying bacteria tended to increase the microbial community stability, indicating a potential coupling between anammox and denitrifying bacteria. Moreover, the nirS-type denitrifiers showed stronger coupling with anammox bacteria than that of nirK-type denitrifiers during the enrichment. Functional potentials as depicted by metagenome sequencing confirmed the ecological interactions between anammox and denitrification. Metagenome-assembled genomes-based ecological model indicated that the most dominant denitrifiers could provide various materials such as amino acid, cofactors, and vitamin for anammox bacteria. Cross-feeding in anammox and denitrifying bacteria highlights the importance of microbial interactions for increasing the anammox N removal in eutrophic lakes. Conclusions This study greatly expands our understanding of cooperation mechanisms among anammox and denitrifying bacteria during the anammox enrichment with eutrophic lake sediments, which sheds new insights into N removal for controlling lake eutrophication.

show abstract

“…With the lower capital investment, less operational maintenance, no additional organic carbon source, 90% reduction of sludge and the limited emission of N 2 O, the anaerobic ammonia oxidation (anammox) process is a new nitrogen removal technology that has gained widespread attention. 1,2 However, despite the increasing interest in the anammox application, there were still many difficulties, such as the low bacterial growth rate and the sensitivity to the environmental factors. 3 Anammox processes can be conducted in many different congurations.…”

Section: Introductionmentioning

confidence: 99%

Investigation of the process stability of different anammox configurations and assessment of the simulation validity of various anammox-based kinetic models

Wang

Zhu

et al. 2020

RSC Adv.

View full text Add to dashboard Cite

show abstract

Accelerated start-up, long-term performance and microbial community shifts within a novel upflow porous-plated anaerobic reactor treating nitrogen-rich wastewater via ANAMMOX process

Abstract: Schematic diagram of the upflow porous-plate anaerobic reactor and nitrogen removal pathways occurring within the reactor.

Cited by 19 publications

References 37 publications

High-quality bacterial genomes of a partial-nitritation/anammox system by an iterative hybrid assembly method

High-quality bacterial genomes of a partial-nitritation/anammox system by an iterative hybrid assembly method

Ecological interactions and the underlying mechanism of anammox and denitrification across the anammox enrichment with eutrophic lake sediments

Investigation of the process stability of different anammox configurations and assessment of the simulation validity of various anammox-based kinetic models

Contact Info

Product

Resources

About