AcCIPK5, a pineapple CBL-interacting protein kinase, confers salt, osmotic and cold stress tolerance in transgenic Arabidopsis

Aslam, Mohammad; Greaves, Joseph G.; Jakada, Bello Hassan; Fakher, Beenish; Wang, Xiaomei; Qin, Yuan

doi:10.1016/j.plantsci.2022.111284

Cited by 22 publications

(13 citation statements)

References 95 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Under salt or drought stress, plants often produce a large amount of reactive oxygen species (ROS), such as superoxide anion (O 2− ) and hydrogen peroxide (H 2 O 2 ) [ 62 , 63 ]. SOD and POD scavenging systems can detoxify ROS to reduce oxidation damage in plant cells and enhance the resistance to stresses [ 64 , 65 ].…”

Section: Discussionmentioning

confidence: 99%

IbMYB308, a Sweet Potato R2R3-MYB Gene, Improves Salt Stress Tolerance in Transgenic Tobacco

Wang

Lei

et al. 2022

Genes

View full text Add to dashboard Cite

The MYB (v-myb avian myeloblastosis viral oncogene homolog) transcription factor family plays an important role in plant growth, development, and response to biotic and abiotic stresses. However, the gene functions of MYB transcription factors in sweet potato (Ipomoea batatas (L.) Lam) have not been elucidated. In this study, an MYB transcription factor gene, IbMYB308, was identified and isolated from sweet potato. Multiple sequence alignment showed that IbMYB308 is a typical R2R3-MYB transcription factor. Further, quantitative real-time PCR (qRT-PCR) analysis revealed that IbMYB308 was expressed in root, stem, and, especially, leaf tissues. Moreover, it showed that IbMYB308 had a tissue-specific profile. The experiment also showed that the expression of IbMYB308 was induced by different abiotic stresses (20% PEG-6000, 200 mM NaCl, and 20% H2O2). After a 200 mM NaCl treatment, the expression of several stress-related genes (SOD, POD, APX, and P5CS) was upregulation in transgenic plants, and the CAT activity, POD activity, proline content, and protein content in transgenic tobacco had increased, while MDA content had decreased. In conclusion, this study demonstrated that IbMYB308 could improve salt stress tolerance in transgenic tobacco. These findings lay a foundation for future studies on the R2R3-MYB gene family of sweet potato and suggest that IbMYB308 could potentially be used as an important positive factor in transgenic plant breeding to improve salt stress tolerance in sweet potato plants.

show abstract

Section: Discussionmentioning

confidence: 99%

IbMYB308, a Sweet Potato R2R3-MYB Gene, Improves Salt Stress Tolerance in Transgenic Tobacco

Wang

Lei

et al. 2022

Genes

View full text Add to dashboard Cite

show abstract

“…Under low-temperature treatment, the expression of LcSnRK3.19 reached its peak 6 hours after low-temperature treatment and then declined but increased after 3 days. In addition, AcCIPK5 , a cognate gene of AtCIPK12 , positively regulated the cold tolerance of A. thaliana in Ananas comosus (L.) [ 65 ]. Consequently, we can conclude that individuals of the SnRK2 and SnRK3 subfamilies are more likely to be interested in the low-temperature response reaction in L. chinense .…”

Section: Discussionmentioning

confidence: 99%

Identification and characteristics of SnRK genes and cold stress-induced expression profiles in Liriodendron chinense

Radani

Ahmad

et al. 2022

BMC Genomics

View full text Add to dashboard Cite

Background The sucrose non-fermenting 1 (SNF1)-related protein kinases (SnRKs) play a vivid role in regulating plant metabolism and stress response, providing a pathway for regulation between metabolism and stress signals. Conducting identification and stress response studies on SnRKs in plants contributes to the development of strategies for tree species that are more tolerant to stress conditions. Results In the present study, a total of 30 LcSnRKs were identified in Liriodendron chinense (L. chinense) genome, which was distributed across 15 chromosomes and 4 scaffolds. It could be divided into three subfamilies: SnRK1, SnRK2, and SnRK3 based on phylogenetic analysis and domain types. The LcSnRK of the three subfamilies shared the same Ser/Thr kinase structure in gene structure and motif composition, while the functional domains, except for the kinase domain, showed significant differences. A total of 13 collinear gene pairs were detected in L. chinense and Arabidopsis thaliana (A. thaliana), and 18 pairs were detected in L. chinense and rice, suggesting that the LcSnRK family genes may be evolutionarily more closely related to rice. Cis-regulation element analysis showed that LcSnRKs were LTR and TC-rich, which could respond to different environmental stresses. Furthermore, the expression patterns of LcSnRKs are different at different times under low-temperature stress. LcSnRK1s expression tended to be down-regulated under low-temperature stress. The expression of LcSnRK2s tended to be up-regulated under low-temperature stress. The expression trend of LcSnRK3s under low-temperature stress was mainly up-or down-regulated. Conclusion The results of this study will provide valuable information for the functional identification of the LcSnRK gene in the future.

show abstract

“…In plants, the calcineurin B-like protein (CBL) family represents a group of calcium sensors and plays a pivotal role in decoding calcium transients by specifically interacting with and regulating a family of protein kinases ( CIPKs ). CIPKs is known to confer cold stress tolerance in cold acclimated Arabidopsis thaliana ( Aslam et al, 2022 ), pepper, and tomato ( Ma et al, 2022 ). Two CIPKs are among the hub genes of WHGs subnet in this study (connection degree = 42) and they are highly expressed in both winter-habit genotypes (WM and N) when treated with cold, but barely any expression in all other samples.…”

Section: Discussionmentioning

confidence: 99%

Computational genomics insights into cold acclimation in wheat

Pan

Liu

et al. 2022

Front. Genet.

View full text Add to dashboard Cite

Development of cold acclimation in crops involves transcriptomic reprograming, metabolic shift, and physiological changes. Cold responses in transcriptome and lipid metabolism has been examined in separate studies for various crops. In this study, integrated computational approaches was employed to investigate the transcriptomics and lipidomics data associated with cold acclimation and vernalization in four wheat genotypes of distinct cold tolerance. Differential expression was investigated between cold treated and control samples and between the winter-habit and spring-habit wheat genotypes. Collectively, 12,676 differentially expressed genes (DEGs) were identified. Principal component analysis of these DEGs indicated that the first, second, and third principal components (PC1, PC2, and PC3) explained the variance in cold treatment, vernalization and cold hardiness, respectively. Differential expression feature extraction (DEFE) analysis revealed that the winter-habit wheat genotype Norstar had high number of unique DEGs (1884 up and 672 down) and 63 winter-habit genes, which were clearly distinctive from the 64 spring-habit genes based on PC1, PC2 and PC3. Correlation analysis revealed 64 cold hardy genes and 39 anti-hardy genes. Cold acclimation encompasses a wide spectrum of biological processes and the involved genes work cohesively as revealed through network propagation and collective association strength of local subnetworks. Integration of transcriptomics and lipidomics data revealed that the winter-habit genes, such as COR413-TM1, CIPKs and MYB20, together with the phosphatidylglycerol lipids, PG(34:3) and PG(36:6), played a pivotal role in cold acclimation and coordinated cohesively associated subnetworks to confer cold tolerance.

show abstract

AcCIPK5, a pineapple CBL-interacting protein kinase, confers salt, osmotic and cold stress tolerance in transgenic Arabidopsis

Cited by 22 publications

References 95 publications

IbMYB308, a Sweet Potato R2R3-MYB Gene, Improves Salt Stress Tolerance in Transgenic Tobacco

IbMYB308, a Sweet Potato R2R3-MYB Gene, Improves Salt Stress Tolerance in Transgenic Tobacco

Identification and characteristics of SnRK genes and cold stress-induced expression profiles in Liriodendron chinense

Computational genomics insights into cold acclimation in wheat

Contact Info

Product

Resources

About