Accumulation of oxidative damage during replicative aging of the yeast Saccharomyces cerevisiae

Grzelak, Agnieszka; Macierzyńska, Ewa; Bartosz, Grzegorz

doi:10.1016/j.exger.2006.06.049

Cited by 22 publications

(15 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This effect coincided with an increase in the presence of oxidized lipids, suggesting that the antioxidant enzymatic capacity of B. bassiana was insufficient to counteract the effects of O 2 modification, especially on lipids. Similarly, Grzelak et al (2006) reported reduction on antioxidant capacity and increase of oxidized biomolecules in S. cerevisiae due to the age of the culture. On the other hand, this oxidant-antioxidant imbalance caused a negative effect on the biomass of B. bassiana, including conidia since germination percentage decreased.…”

Section: Discussionmentioning

confidence: 94%

“…It has also been reported that the amount of accumulated carbonyl groups increases in Saccharomyces cerevisiae at senescence, which was linked to the fact that antioxidant capacity diminishes at this point in comparison to other stages of growth (Grzelak et al 2006). Furthermore, thermal shock induction in A. niger produced oxidative stress, such that Cat activity diminished at the end of the culture period, and protein oxidation consequently increased (Abrashev et al 2008).…”

Section: Discussionmentioning

confidence: 97%

“…The assay is based on the derivatization and quantification of oxidized proteins using 2, 4-dinitrophenylhydrazine (DNPH) (Gocheva et al 2009;Grzelak et al 2006). This variable was reported in terms of nanomols of carbonyl groups per milligram of protein (nmol carbonyls mg -1 protein).…”

Section: Oxidized Proteinmentioning

confidence: 99%

See 2 more Smart Citations

Physiological and antioxidant response by Beauveria bassiana Bals (Vuill.) to different oxygen concentrations

Garza-López

Königsberg

Gómez-Quiroz

et al. 2011

World J Microbiol Biotechnol

View full text Add to dashboard Cite

The effect of three levels of oxygen (normal atmosphere (21% O(2)), low oxygen (16% O(2)) and enriched oxygen (26% O(2))) on the production and germination of conidia by Beauveria bassiana was evaluated using rice as a substrate. The maximum yield of conidia was achieved under hypoxia (16% O(2)) after 8 days of culture (1.51 × 10(9) conidia per gram of initial dry substrate), representing an increase of 32% compared to the normal atmosphere. However, germination was reduced by at least 27% due to atmospheric modifications. Comparison of antioxidant enzyme activity (superoxide dismutases and catalases) with the oxidation profiles of biomolecules (proteins and lipids) showed that a decrease in catalase activity in the final days of culture coincided with an increase in the amount of oxidized lipids, showing that oxidative stress was a consequence of pulses of different concentrations of O(2). This is the first study describing oxidative stress induction by atmospheric modification, with practical implications for conidia production.

show abstract

Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 97%

See 1 more Smart Citation

Physiological and antioxidant response by Beauveria bassiana Bals (Vuill.) to different oxygen concentrations

Garza-López

Königsberg

Gómez-Quiroz

et al. 2011

World J Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…Важливим показником оксидативного/ карбонільного стресу та старіння, який також залежить від особливостей росту дріжджів, є вміст карбонільних груп протеїнів [16,22,[37][38][39][40][41][42]. На рис.…”

Section: рис 2 криві росту S Cerevisiae Yph250 у живильному середоunclassified

Defects in antioxidant defence enhance glyoxal toxicity in the yeast Saccharomyces cerevisiae

Semchyshyn¹

2013

Ukr.Biochem.J.

View full text Add to dashboard Cite

“…Studies have demonstrated that replicative senescence of yeast cells involves a collapse of the antioxidant defense mechanisms and accumulation of oxidative damage to cellular components [4]. DNA integrity and stability of an organism is fundamental for survival, even under the best circumstances DNA is continuously damaged by endogenous or exogenous genotoxic agents [5,6].…”

Section: Introductionmentioning

confidence: 99%

Absence of superoxide dismutase activity causes nuclear DNA fragmentation during the aging process

Muid

Karakaya

Koç

2014

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

a b s t r a c tSuperoxide dismutases (SOD) serve as an important antioxidant defense mechanism in aerobic organisms, and deletion of these genes shortens the replicative life span in the budding yeast Saccharomyces cerevisiae. Even though involvement of superoxide dismutase enzymes in ROS scavenging and the aging process has been studied extensively in different organisms, analyses of DNA damages has not been performed for replicatively old superoxide dismutase deficient cells. In this study, we investigated the roles of SOD1, SOD2 and CCS1 genes in preserving genomic integrity in replicatively old yeast cells using the single cell comet assay. We observed that extend of DNA damage was not significantly different among the young cells of wild type, sod1D and sod2D strains. However, ccs1D mutants showed a 60% higher amount of DNA damage in the young stage compared to that of the wild type cells. The aging process increased the DNA damage rates 3-fold in the wild type and more than 5-fold in sod1D, sod2D, and ccs1D mutant cells. Furthermore, ROS levels of these strains showed a similar pattern to their DNA damage contents. Thus, our results confirm that cells accumulate DNA damages during the aging process and reveal that superoxide dismutase enzymes play a substantial role in preserving the genomic integrity in this process.

show abstract

Accumulation of oxidative damage during replicative aging of the yeast Saccharomyces cerevisiae

Cited by 22 publications

References 37 publications

Physiological and antioxidant response by Beauveria bassiana Bals (Vuill.) to different oxygen concentrations

Physiological and antioxidant response by Beauveria bassiana Bals (Vuill.) to different oxygen concentrations

Defects in antioxidant defence enhance glyoxal toxicity in the yeast Saccharomyces cerevisiae

Absence of superoxide dismutase activity causes nuclear DNA fragmentation during the aging process

Contact Info

Product

Resources

About