The rise in contaminated sites presents a significant issue for the environment and human health, necessitating the decontamination of the surroundings and the adoption of effective decontamination strategies. This investigation was initiated to assess the potential aspects of Acacia karroo in conjunction with enzyme activity, a method that shows promise for mitigating soil contamination. Acacia karroo, with its hyperaccumulator traits, demonstrates great capacity. Enzymes significantly efficiently convert and detoxify harmful substances to a non-toxic level. ICP-MS quantified the concentrations of trace elements in Acacia karroo, while colorimetric assays were used to determine the activity levels of the enzymes. Ten toxic elements were identified in leaf samples of Acacia karroo in the following sequence: Sr > Zn > Cr > V > Rb > Cu > Ni > Y > Sc > Co; concentrations ranged between 203.86 ± 4.48 ppm (Zn) and 10.12 ± 0.09 ppm (Sc). The concentration of these metals was very high, posing a potential risk of harming the environment. Meanwhile, the three identified enzymes, invertase (INV), phosphatase (PHO), and catalase (CAT), have high and average activity levels, respectively. PHO and CAT showed a positive correlation with Zn, Rb, Sr, and Y, while INV correlated positively with Sc, V, Cr, Co, Cu, and Ni content. The principal component analysis (PCA) findings in this study demonstrated an inconclusive correlation between soil enzyme activity and soil heavy metal content. Both positive and negative correlations between soil enzyme activity and heavy metals were observed. This investigation revealed Acacia karroo as an optimal botanical species for phytoremediation. Consequently, a correlation analysis demonstrated that incorporating the Acacia karroo species along with enzyme activity seems to be a highly promising environmentally friendly technique for remediating soil pollution. The Acacia species can also be used in phytoremediation efforts to help conserve biodiversity. Subsequent investigations should focus on the operational mechanisms of different plant parts used as herbal remedies, isolated compounds, their efficacy, adverse effects, and practical implications.