Accumulibacter clades Type I and II performing kinetically different glycogen-accumulating organisms metabolisms for anaerobic substrate uptake

Welles, Laurens; Tian, Wen‐de; Saad, Sondos A.; Abbas, Ben; López-Vázquez, Carlos M.; Hooijmans, Christine M.; Loosdrecht, M.C.M. van; Brdjanović, Damir

doi:10.1016/j.watres.2015.06.045

Cited by 117 publications

(71 citation statements)

References 47 publications

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“…GAO compete with PAO for substrate but neither release phosphate anaerobically nor store it under aerobic conditions. Besides GAO, the enrichment of different PAO clades is another factor since PAO I mainly relies on poly-P as energy source for VFA uptake, while PAO II utilizes a mixed PAO-GAO metabolism where glycogen generates a significant part of the energy required for VFA uptake (Welles et al, 2015b). Regarding the operational conditions, pH, nature of the carbon source, sodium and calcium concentration affect the anaerobic stoichiometry (Satoh et al, 1992; Smolders et al, 1994; Oehmen et al, 2005; Barat and van Loosdrecht, 2006; Barat et al, 2008; Welles et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…In this regard, at short-term, enriched PAO cultures can shift from a poly-P dependent toward a glycogen dependent metabolism when the biomass P-content decreases (Brdjanovic et al, 1998; Hesselman et al, 2000; Erdal et al, 2008; Zhou et al, 2008; Acevedo et al, 2012). Using highly enriched PAO I and II cultures, Welles et al (2015b) demonstrated that the poly-P depletion led to a seven and two-fold decrease in the anaerobic kinetic rates of PAO I and II, respectively. Although these studies clearly indicated that the PAO possess metabolic flexibility, the results only represent the short-term response to changes in the biomass P-content.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, in most of the studies the specific PAO clades were either not reported or the biomass consisted of a mixture of different PAO clades (Brdjanovic et al, 1998; Hesselman et al, 2000; Zhou et al, 2008; Erdal et al, 2008; Acevedo et al, 2012). Finally, not all studies covered a broad range of different biomass P-contents (Welles et al, 2015b). Therefore, it remains unclear how the kinetic rates and stoichiometry of specific PAO clades I and II are affected by a wide range of different biomass P-contents during long-term operation.…”

Section: Introductionmentioning

confidence: 99%

“…Kong et al (2002), in a similar study, observed a shift from Betaproteobacteria (the subdivision to which most “ Candidatus Accumulibacter phosphatis” belong) at a high P/C influent ratio to Alphaproteobacteria (the subdivision to which “ Defluviicoccus ” belong) and Gammaproteobacteria (the subdivisions to which most “ Candidatus Competibacter phosphatis” belong) at a low P/C influent ratio. Recent studies have demonstrated that PAO I and II have very different characteristics in terms of morphology, stoichiometry and kinetic rates, the ability to denitrify and possibly tolerance to stress conditions as well (Carvalho et al, 2007; Flowers et al, 2009; Slater et al, 2010; Welles et al, 2015b). These differences may lead to the prevalence of specific clades under certain conditions and consequently differences in the metabolic conversions.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore it becomes important to study the metabolism of the specific clades separately to get a better understanding about the conditions favoring the specific clades. Furthermore, considering the large differences in kinetic rates (four times higher for PAO II at poly-P depleted conditions), the different PAO clades can no longer be considered as one organism in modeling approaches (Welles et al, 2015b). …”

Section: Introductionmentioning

confidence: 99%

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Metabolic Response of “Candidatus Accumulibacter Phosphatis” Clade II C to Changes in Influent P/C Ratio

et al. 2017

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The objective of this study was to investigate the ability of a culture highly enriched with the polyphosphate-accumulating organism, “Candidatus Accumulibacter phosphatis” clade IIC, to adjust their metabolism to different phosphate availabilities. For this purpose the biomass was cultivated in a sequencing batch reactor with acetate and exposed to different phosphate/carbon influent ratios during six experimental phases. Activity tests were conducted to determine the anaerobic kinetic and stoichiometric parameters as well as the composition of the microbial community. Increasing influent phosphate concentrations led to increased poly-phosphate content and decreased glycogen content of the biomass. In response to higher biomass poly-phosphate content, the biomass showed higher specific phosphate release rates. Together with the phosphate release rates, acetate uptake rates also increased up to an optimal poly-phosphate/glycogen ratio of 0.3 P-mol/C-mol. At higher poly-phosphate/glycogen ratios (obtained at influent P/C ratios above 0.051 P-mol/C-mol), the acetate uptake rates started to decrease. The stoichiometry of the anaerobic conversions clearly demonstrated a metabolic shift from a glycogen dominated to a poly-phosphate dominated metabolism as the biomass poly-phosphate content increased. FISH and DGGE analyses confirmed that no significant changes occurred in the microbial community, suggesting that the changes in the biomass activity were due to different metabolic behavior, allowing the organisms to proliferate under conditions with fluctuating phosphate levels.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Metabolic Response of “Candidatus Accumulibacter Phosphatis” Clade II C to Changes in Influent P/C Ratio

et al. 2017

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Modeling hydraulic transport and anaerobic uptake by PAOs and GAOs during wastewater feeding in EBPR granular sludge reactors

Weissbrodt

Holliger

Morgenroth

2017

Biotech & Bioengineering

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New-generation bioprocesses using granular sludge aim for a high-rate removal of nutrients from wastewater with low footprint. Achieving enhanced biological phosphorus removal (EBPR) relies on the design of sludge beds and wastewater feeding conditions to optimally load the biomass and to select for polyphosphate- (PAOs) over glycogen-accumulating organisms (GAOs) and over other heterotrophs. A hydraulic-metabolic mathematical model was developed to elucidate the impact of hydraulic transport patterns and environmental conditions on the PAO/GAO competition during up-flow feeding through an EBPR granular sludge bed. Tracer experiments highlighted plug-flow regimes with dispersion under both rapid (9 m h , Re = 1.6, Pe = 7.2, Pe = 4.6) and slow (0.9 m h , Re = 0.2, Pe = 21.3, Pe = 3.4) feeding. Non-turbulent regimes (Re << 10 ) promote a safe implementation of simultaneous fill/draw. Feeding time, pH, and temperature significantly impacted bacterial competition for carbon uptake under anaerobic slow feeding. Feeding duration should be designed to avoid full depletion of intracellular storage polymers within static granules. PAOs bear twice longer feeding than GAOs by using both polyphosphate and glycogen hydrolysis to sustain anaerobic C-uptake. Alkaline conditions (pH 7.25-8.0) by, e.g., dosing lime in the feed select for PAOs independently of temperature (10-30°C). A twice higher bed is required for full anaerobic conversions at 10 rather than 20°C. Biosystem responses for anaerobic C-uptake can be anticipated using the model toward designing robust anaerobic selectors to manage the microbial resource in EBPR granular sludge. Biotechnol. Bioeng. 2017;114: 1688-1702. © 2017 Wiley Periodicals, Inc.

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Granular Sludge—State of the Art

Weissbrodt

2024

Springer Theses

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Accumulibacter clades Type I and II performing kinetically different glycogen-accumulating organisms metabolisms for anaerobic substrate uptake

Cited by 117 publications

References 47 publications

Metabolic Response of “Candidatus Accumulibacter Phosphatis” Clade II C to Changes in Influent P/C Ratio

Metabolic Response of “Candidatus Accumulibacter Phosphatis” Clade II C to Changes in Influent P/C Ratio

Modeling hydraulic transport and anaerobic uptake by PAOs and GAOs during wastewater feeding in EBPR granular sludge reactors

Granular Sludge—State of the Art

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