Accuracy comparison study of new smartphone-based semen analyzer versus laboratory sperm quality analyzer

Park, Min Jung; Lim, Mi Young; Park, Hyun Jun; Park, Nam Cheol

doi:10.4111/icu.20210266

Cited by 11 publications

(4 citation statements)

References 16 publications

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“…These results are also consistent with others in the literature, such as the findings of 98.04% and 93.94% related to an untrained user performing analysis with a similar device, as derived by Kanakasabapathy et al [19]. On the other hand, our diagnostic accuracy in relation to asthenozoospermia was better than the findings of Park et al [29], who described sensitivity and specificity values for their smartphone-based analyzer when assessing sperm motility of 92.6% and 66.7%, respectively. Further, we evaluated the efficacy and applicability of SpermCell™, and found that test results obtained from lay patients were statistically similar to those obtained from trained laboratory staff.…”

Section: Discussionsupporting

confidence: 93%

“…Further, in 2018, the accuracy of another device was reported as 97.80% for motile sperm count in 144 semen samples from 24 healthy men (Agarwal et al) [27]. On the other hand, for one of the most recently developed smartphone-based semen analysis devices, the overall accuracy was reported as 84.6% in detecting sperm motility (2021) [29]. The diagnostic sensitivity and specificity of SpermCell TM -based semen analyses were also considerable when using them to determine oligospermia and asthenozoospermia.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Introducing a New Smartphone Applied Semen Analyzer, SpermCell™: A Cross-Sectional Validation Study with a Comparative Analysis and a Mini Patient Questionnaire on a Large Sample Cohort

Dincer,

Yentur,

Colakerol

et al. 2024

Diagnostics

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(1) Background: Standard semen analysis methods may exhibit variability between observers and/or human error; therefore, additional methods are needed to overcome these handicaps. We aimed to present a new smartphone-applied semen analyzer, Sperm Cell™, investigate its diagnostic efficacy by comparing it with the standard analysis method, and determine its user-friendly nature. (2) Methods: A cross-sectional study was conducted on a large sample cohort, including 102 men. Three semen analyses were performed for each semen sample. The first employed the standard manual method, whereas the others were smartphone-based analyses performed by technicians and patients. We compared major semen parameters between the three semen analyses. The user-friendly nature of the analyzer was also evaluated with a mini-questionnaire completed by the participants. (3) Results: The determined median sperm count, motile sperm count, and percentage of motile sperms, on standard manual semen analysis, were 50.00 × 106/mL (0–160 × 106/mL), 23.94 × 106/mL (0–108 × 106/mL) and 50.00% (0–73.00%), respectively. Median sperm count and motile sperm count were 50.52 × 106/mL (<1–150 × 106/mL) vs. 55.77 × 106/mL (<1–160 × 106/mL) and 23.34 × 106/mL (0–105 × 106/mL) vs. 23.53 × 106/mL (0–104 × 106/mL) for SpermCell™-based semen analysis performed by a technician and patients themselves, respectively. The percentages of motile sperms were 47.40% (0–67.00%) vs. 47.61% (0–80.20%), respectively. All the parameters were statistically similar between the three semen analysis methods (p > 0.05 for each). The SpermCell™ analysis results were correlated with the standard manual method with up to 0.85 correlation coefficients. Moreover, substantial diagnostic accuracy, sensitivity and specificity were obtained in determining the oligospermia and asthenozoospermia via the device-based analyses performed by technician and patients. The mini-questionnaire results revealed that the analyzer is useful. (4) Conclusions: The novel smartphone-applied semen analyzer is a helpful tool with acceptable diagnostic accuracy in determining the major semen parameters. It can be used as an efficient at-home point-of-care testing method in the initial assessment of couples with infertility concerns.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Introducing a New Smartphone Applied Semen Analyzer, SpermCell™: A Cross-Sectional Validation Study with a Comparative Analysis and a Mini Patient Questionnaire on a Large Sample Cohort

Dincer,

Yentur,

Colakerol

et al. 2024

Diagnostics

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“…The costs are further reduced when additional PDMS casts are performed and when considering that microscope slides and coverslips are reusable. Additional modifications can lead to smart-phone integration for inexpensive and mobile sperm analysis without the need for bulky lab equipment [44].…”

Section: Discussionmentioning

confidence: 99%

Development of a Single-Piece Sperm Counting Chamber (SSCC) for Aquatic Species

Belgodere

Liu

Reich

et al. 2022

Fishes

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Accurate determination of sperm concentration in aquatic species is important for assisted reproduction and cryopreservation, yet is challenging as current counting methods are costly or not suitable for many species. The goal of this work was to develop a simple (single-piece and single-layer photolithography) sperm counting chamber (SSCC) for aquatic species. Goldfish (Carassius auratus) and zebrafish (Danio rerio) sperm were used for evaluation in the device, which was created with soft lithography. Four designs with different geometries were evaluated for counting accuracy. Open-corner and open-midpoint designs were the most accurate with no significant differences (P > 0.05) for most of the target sperm concentrations (0.5–1.0 × 108 cells/mL). The open-corner design was not significantly different from the Makler® counting chamber intended for human sperm cells (P = 0.6) but was significantly different from a hemocytometer (P < 0.001) intended for other cell sizes. Material cost of device production was USD 16 per unit, including photolithography supplies, glass slide and coverslip, and polydimethylsiloxane. The cost can be reduced to USD 2 per unit with repeated wafer casts. This device could be further refined for resin 3-D printing and sharing via open-hardware approaches and modified to best suit species specific applications.

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“…Besides the iSperm ® , other portable devices have been validated for the analysis of human and domestic animal samples. Smartphone-based semen analyzers, for instance, showed strong correlations with computerized systems and microscopic assessment, even when used by non-professionals for at-home assessment [ 34 , 35 ]. Similarly, the Ongo Sperm Test ® (Microfluidlabs, Budapest, Hungary) was found a good alternative to conventional CASA system in stallions and boars [ 16 , 36 ].…”

Section: Discussionmentioning

confidence: 99%

Sperm Gone Smart: A Portable Device (iSperm®) to Assess Semen Concentration and Motility in Dogs

Domain

Banchi

Hassan

et al. 2022

Animals

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The iSperm® is a portable device for semen analysis. This study aimed to investigate its correlation with a conventional computer-assisted sperm analyzer (ISAS®v1) for the assessment of semen concentration and kinematic parameters in dogs (n = 224). The intra-assay variability of both devices and their ability to estimate semen concentration at a fixed value of 40 × 106/mL were also investigated. Results showed that the intra-assay variability was lower for the ISAS®v1 for all parameters compared to the iSperm®. Hence, iSperm® estimates were more variable in-between fields. Both the iSperm® and the ISAS®v1 were not reliable in estimating semen concentration (ISAS®v1: median 30 × 106/mL, interquartile range (IQR) 12, p < 0.01; iSperm®: median 35.12 × 106/mL, IQR 11.11, p < 0.01). Finally, positive correlations were found between both devices with stronger correlations obtained when four fields were analyzed by the iSperm®. However, the low number of spermatozoa analyzed per field and the inability to avoid artifacts are downsides that currently limit the reliability of the iSperm®. Therefore, the software of iSperm® needs some improvement to make it a valid and practical alternative to automated computerized systems for the analysis of canine semen.

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Accuracy comparison study of new smartphone-based semen analyzer versus laboratory sperm quality analyzer

Cited by 11 publications

References 16 publications

Introducing a New Smartphone Applied Semen Analyzer, SpermCell™: A Cross-Sectional Validation Study with a Comparative Analysis and a Mini Patient Questionnaire on a Large Sample Cohort

Introducing a New Smartphone Applied Semen Analyzer, SpermCell™: A Cross-Sectional Validation Study with a Comparative Analysis and a Mini Patient Questionnaire on a Large Sample Cohort

Development of a Single-Piece Sperm Counting Chamber (SSCC) for Aquatic Species

Sperm Gone Smart: A Portable Device (iSperm®) to Assess Semen Concentration and Motility in Dogs

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