Accuracy of a rapid real-time polymerase chain reaction assay for diagnosis of group B <i>Streptococcus</i> colonization in a cohort of HIV-infected pregnant women

Gouvêa, Maria Isabel Fragoso da Silveira; Joao, Esau C; Teixeira, Maria de Lourdes Benamor; Read, Jennifer S.; Fracalanzza, Sérgio Eduardo Longo; Souza, Cláudia Teresa Vieira de; Souza, Maria José de; Filho, Helio M Torres; Leite, Cassiana C. F.; Brasil, Pedro Emmanuel Alvarenga Americano do

doi:10.1080/14767058.2016.1205021

Cited by 9 publications

(5 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Xpert GBS and GBS LB (Cepheid, USA) are rapid and convenient PCR assays for detecting GBS based on Cepheid’s Gene Xpert System [ 11 ]. A comprehensive search based on the inclusion and exclusion criteria set by this study led to the retrieval of 13 articles and 19 sets of data.…”

Section: Discussionmentioning

confidence: 99%

“…However, with limitations: (a) it requires 18–72 h; (b) the source of specimen collection and the experience of laboratory operators have a significant impact on its accuracy; (c) sensitivity is estimated at 54.3 to 83.3%. In addition, as enrichment culture takes time to obtain results, some pregnant women who don't receive prenatal care before presenting in labor and those who give birth prematurely, before the recommended testing period cannot be helped [ 10 , 11 ]. Moreover, the 2010 CDC revision of the guidelines mentioned that nucleic acid amplification tests (NAAT) such as polymerase chain reaction (PCR) could be used to assess intrapartum GBS colonization with the advantage of rapid turnaround time.…”

Section: Introductionmentioning

confidence: 99%

“…Presently, increasing real-time PCR assays for GBS have been developed, such as Xpert GBS (Cepheid, USA) and Xpert GBS LB (Cepheid, USA). Cepheid’s Gene Xpert System can automatically extract, amplify, and detect DNA, a system that could be installed out of the laboratory in countries approved by regulatory bodies, only requiring simple operation and providing results in less than 1 h, resulting in a more practical method for the rapid diagnosis of GBS [ 11 , 13 ]. It targets the CAMP factor encoding gene present in nearly all GBS [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of Xpert GBS assay and Xpert GBS LB assay for detection of Streptococcus agalactiae

Han

Xie

Huang

et al. 2021

Ann Clin Microbiol Antimicrob

View full text Add to dashboard Cite

Background Group B Streptococcal (GBS) infection is the primary agent of neonatal morbidity and mortality. Rapid and simple methods to detect GBS are Xpert GBS and GBS LB assays based on real-time polymerase chain reaction (PCR). However, since the diagnostic accuracy of the two techniques in diagnosing GBS remains unclear, we designed this study to appraise the diagnostic accuracy of the aforementioned. Methods A systematic search of all literature published before July 16, 2020 was conducted using Embase, PubMed, Web of Science, and Cochrane Library. The study quality was evaluated through Review Manager 5.3. Accordingly, data extracted in the included studies were analyzed using Meta-DiSc 1.4 and Stata 12.0 software. The diagnosis odds ratio (DOR) and bivariate boxplot were utilized to evaluate the heterogeneity. Publication bias was appraised by using Deeks’ funnel plot. Results A total of 13 studies were adopted and only 19 sets of data met the criteria. The sensitivity and specificity of Xpert GBS were 0.91 (95% CI 0.89–0.92) and 0.93 (95% CI 0.92–0.94). The area under the curve (AUC) was 0.9806. The sensitivity and specificity results of Xpert GBS LB were 0.96 (95% CI 0.95–0.98) and 0.94 (95% CI 0.92–0.95), respectively. The AUC was 0.9950. No publication bias was found. Conclusions The Xpert GBS and GBS LB assays are valuable alternative methods with high sensitivity and specificity. However, determining whether they can be used as clinical diagnostic standards for GBS is essential for the future.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of Xpert GBS assay and Xpert GBS LB assay for detection of Streptococcus agalactiae

Han

Xie

Huang

et al. 2021

Ann Clin Microbiol Antimicrob

View full text Add to dashboard Cite

show abstract

“…We understand that the use of non-selective medium is one of the limitations in this study, because GBS selectivity may be reduced by the overgrowth of other bacterial species other than GBS during the culture process [ 10 ]. However, if a priority is given to the comfort of pregnant women, further studies such as real-time polymerase chain reaction (PCR) assay for predicting colonization of GBS at labor may be needed [ 11 ]. Otherwise, a further large prospective study may be needed to compare the accuracy of both specimens to deny the necessity of anorectal culture of GBS.…”

Section: To the Editormentioning

confidence: 99%

Low Accuracy of Antenatal Screening for Group B Streptococcus From Perianal Area

Suzuki

2023

J Clin Med Res

View full text Add to dashboard Cite

“…Therefore, further studies such as real-time PCR assay for predicting colonization of GBS at labor may be needed. [19] Table 2 shows the 12 cases of neonatal GBS infection resulted in CP. There were 8 and 4 cases of early and lateonset type GBS infection, respectively.…”

Section: Brue/altementioning

confidence: 99%

Emergent serious condition of the neonates associated with cerebral palsy in Japan

Suzuki

2022

PRM

View full text Add to dashboard Cite

Objective: To analyze the emergent serious conditions contributing to cerebral palsy (CP) of neonates without a history of the necessity of neonatal resuscitation within 5 minutes after delivery in Japan. Methods: To examine the clinical courses regarding CP cases, we reviewed the summary reports of antenatal- and peripartum CP cases published on the home page of the Japan Obstetric Compensation System for Cerebral Palsy (JOCSC) launched in 2009. Results: Between January 2009 and February 2016, in Japan there were 51 cases analyzed as CP due to emergent serious condition of the neonates without a history of necessity of neonatal resuscitation within 5 minutes after delivery at the Japan Obstetric Compensation System for CP. The main pathological conditions described as the cause of CP were as follows: the concept of brief resolved unexplained events/apparent life-threatening event (n = 15), neonatal Group B streptococcus infection (n = 12), herpes simplex virus infection (n = 6), neonatal hypoglycemia (n = 3), and neonatal hyperkalemia (n = 3). Conclusions: Based on the current cases, we should note that neonates are very unstable, especially in the early period after delivery.

show abstract

Accuracy of a rapid real-time polymerase chain reaction assay for diagnosis of group B Streptococcus colonization in a cohort of HIV-infected pregnant women

Abstract: GeneXpert GBS is an acceptable test for the identification of GBS colonization in HIV-infected pregnant women and represents a reasonable option to detect GBS colonization in settings where culture is not feasible.

Cited by 9 publications

References 23 publications

Evaluation of Xpert GBS assay and Xpert GBS LB assay for detection of Streptococcus agalactiae

Evaluation of Xpert GBS assay and Xpert GBS LB assay for detection of Streptococcus agalactiae

Low Accuracy of Antenatal Screening for Group B Streptococcus From Perianal Area

Emergent serious condition of the neonates associated with cerebral palsy in Japan

Contact Info

Product

Resources

About