Accuracy of First and Second Generation Testosterone Assays and Improvement through Sample Extraction

Comparison of 7 Published LC-MS/MS Methods for the Simultaneous Measurement of Testosterone, Androstenedione, and Dehydroepiandrosterone in Serum

“…2 shows the variation between the immunoassays. This high variation among immunoassays was not caused by 1 of the methods out- lying the others, but rather represents the general variation of these immunoassays in the female testosterone concentration range (11 ). This difference in the variation between LC-MS/MS methods and immunoassays indicates that, mainly at low testosterone concentrations, the LC-MS/MS methods used in our study as a group were in better agreement with each other than the immunoassays.…”

“…The currently used second-and third-generation testosterone assays generally show a clear improvement in the lower concentration range compared to the first-generation assays investigated by Taieb et al (1,11,12 ). For this reason, when investigating the potential superiority of mass spectrometry-based testosterone assays, the performance of the currently used immunoassays should be taken into account.…”

“…We investigated imprecision and agreement among the published LC-MS/MS methods for the simultaneous measurement of testosterone, androstenedione, and DHEA. In addition, the variation among the investigated LC-MS/MS testosterone assays was compared with the variation among currently used immunoassays for measurement of testosterone (11 ).…”

Restoring testosterone levels by adding dehydroepiandrosterone to a drospirenone containing combined oral contraceptive: I. Endocrine effects

“…Accurate measurement of free T in blood of females is difficult [26,27] due to the very low concentrations (35 -700 pmol/L; [24]), especially in women using COCs [1].…”

Complex Biological Pattern of Fertility Hormones in Children and Adolescents: A Study of Healthy Children from the CALIPER Cohort and Establishment of Pediatric Reference Intervals

“…Over the last 10 years, immunoassay-based measurements of endocrine markers have improved substantially, and new technological advances have resulted in very sensitive and robust methods with increased specificity (9,10 ). However, many existing reference intervals are based on older methods (7 ) and are therefore outdated.…”