Accuracy of Quantitative Paper Chromatography in Amino Acid Determination Using Direct Photometry

Roberts, Henry R.; Kolor, Michael G.

doi:10.1021/ac60132a040

Cited by 21 publications

(6 citation statements)

References 19 publications

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“…by Roberts and Kolor (22) for a maximum density method using seven solvents. Tyrosine shows a relatively high coefficient of variation because it streaks on chromatograms and reacts poorly with ninhydrin (Table IV).…”

Section: Resultsmentioning

confidence: 99%

Determination of Amino Acids from Plants by Paper Chromatography

Thompson¹,

Morris²

1959

Anal. Chem.

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potassium was found in the basic amino acid fraction and no detectable amount with the other amino acids. In obtaining the amine fraction, the monovalent cations are removed from the resin with 0.5V hydrochloric acid, but the diand trivalent cations are only partially eluted. The remaining polyvalent cations which are eluted with 6Ar hydrochloric acid usually do not interfere with the chromatography of amines.The acid form of Dowex 50 does not retain strongly acidic amino acids such as cysteic acid and taurine (3) and these are not purified. Such strongly acidic amino acids have not been reported in plants (20). It would be possible to retain these acids and other anions on the salt form of a strongly basic resin while nonionic substances would pass through. Such variations would provide a basis for a more extensive fractionation scheme whereby separate fractions with nonionic materials, anions, neutral amino acids, acidic amino acids, basic amino acids, cations, and some amines could be obtained (10, 17).

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Section: Resultsmentioning

confidence: 99%

Determination of Amino Acids from Plants by Paper Chromatography

Thompson¹,

Morris²

1959

Anal. Chem.

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“…(A Photovolt densitometer Model 525 was used in this work.) Measuring the maximum color density of the individual amino acid spots (9,12) was found satisfactory. When this technique is used, the absorbance of the unknown must fall within the absorbance range of the known solution.…”

Section: Samplementioning

confidence: 97%

Rapid Quantitative Determination of Amino Acids by High Temperature Paper Chromatography

Himes¹,

Metcalfe²

1959

Anal. Chem.

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“…The intensities of the colored sugar spots were read at 445 µ on the densitometer with a slit width of 1 mm. Measuring the maximum color density of the individual sugar spots (5,6) was found satisfactory. When this technique is used, the color density of the unknown must fall within the density color range of the knowm solution.…”

Section: Hi Fructosementioning

confidence: 97%

“…The progress of a separation is controlled by the proportioning pump because the reservoir, constant-volume mixing chamber, and column are connected in series to the pump. The rapid response of the instrument permits the use of exponential elution gradients (6) which accomplish an analytical separation of condensed phosphate species in a minimum amount of time. A spare poly (vinyl chloride) tube is installed in the proportioning pump to facilitate regeneration and re-equilibration of an "off-stream" column.…”

Section: Adaptation Of Instrument To Ion Exchange Chromatographymentioning

confidence: 99%