Accurate and rapid detection of <i>Fasciola hepatica</i> copro‐DNA in sheep using loop‐mediated isothermal amplification (LAMP) technique

Amiri, Siamak; Shemshadi, Bahar; Shirali, Saloomeh; Kheirandish, Farnaz; Fallahi, Shirzad

doi:10.1002/vms3.455

Cited by 13 publications

(16 citation statements)

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“…There was no cross-reactivity and the specificity of PCR and RPA was 100% ( 36 ). Besides, there was kappa agreement (0.72) among microscopic diagnostic tests, PCR and loop-mediated isothermal amplification (LAMP), based on IGS and examine on stool sheep samples, and the LAMP could be used in the field conditions of fascioliasis in animals ( 37 ). The results of another study showed that the subjects who were positive for ELISA, their stool and urine samples were 96% and 90% positive by nested PCR (use of ITS1 such as present study) respectively, and only 20% of samples was diagnosed by parasitology method ( 13 ).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Semi-Nested PCR Compared with Indirect-ELISA to Diagnose Human Fasciolosis

Alizadeh¹,

Aryaeipour²,

Mohebali³

et al. 2022

ijph

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Background: We aimed to compare semi-nested PCR with indirect ELISA to diagnose human fasciolosis. Methods: Overall, 70 serum samples were collected from different areas in Iran suspected for fascioliasis. Individuals were classified based on diagnostic of fascioliasis and habitat in endemic areas. Finally, all serum samples were tested by indirect ELISA (using secretory excretory antigen) and semi-nested PCR (using ITS1 gene). The study was conducted in the School of Publish Health, Tehran University of Medical Sciences, Iran in 2021. Results: Significant differences were found between agreement and similarity of patients' results of indirect ELISA and semi-nested PCR 94.46% and 98.4% respectively (Cohen's kappa ≥0.6; P-value≤0.05). No cross-reactions were observed with other parasitic diseases (toxocariasis, hydatidosis, strongyloidiasis, toxoplasmosis, cutaneous leishmaniasis, taeniasis and trichinosis). 69.84% of samples were positive by both techniques. In addition, the percentage of agreement and similarity between the results of the two techniques based on habitat in endemic areas was 88.9-100% and 97.7-100%, respectively (Cohen's kappa ≥0.6; P-value≤0.05). Conclusion: Semi-nested PCR could be a suitable method for following up on patients' treatment and a confirmatory method for ELISA as for diagnosis of human fascioliasis.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Semi-Nested PCR Compared with Indirect-ELISA to Diagnose Human Fasciolosis

Alizadeh¹,

Aryaeipour²,

Mohebali³

et al. 2022

ijph

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“…Для подтверждения диагноза большинства трематодных инвазий используется микроскопия образцов стула пациента, так как метод является простым и доступным в выполнении, однако, позволяет выявить только инвазию средней и высокой интенсивности [12,16]. Большей чувствительностью и универсальностью при низкой интенсивности инвазии обладает метод седиментации [15,17,18]. Микроскопия образцов фекалий, мочи или дуоденального содержимого требует обеспечение лаборатории световым микроскопом и квалицированным опытным персоналом, что не всегда возможно в эндемичных очагах трематодозов.…”

Section: современные технологии микроскопииunclassified

“…К тому же визуализация результата тоже достаточно проста -колориметрическое изменение раствора наблюдается под ультрафиолетом. Схожие результаты были представлены в исследовании сравнения классических паразитологических исследований (прямой влажный препарат и метод концентрации), методов ПЦР и LAMP для диагностики F. hepatica в образцах фекалий овец [18]. Также LAMP-анализ позволяет избежать перекрестных реакций с другими видами.…”

Section: молекулярно-генетические методыunclassified

Foresight in the diagnosis of trematodiasis: innovations versus routine methods

et al. 2023

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Aim. To analyze modern methods for the diagnosis of trematodiasis in experimental and epidemiological studies.Trematodiasis is a group of common parasitic diseases that are a socially sensitive health problem worldwide. According to World Health Organization, more than 250 million people are affected by trematode infections globally. The most common types of human trematode infections are diseases caused by Schistosoma, Fasciola, Clonorchis, and Opisthorchis pathogens. Diagnosis of trematodiasis is often multistage and includes identification of disease symptoms, analysis of medical history, and use of various laboratory tests. Clinical presentation of parasitic infections often varies, making a definitive diagnosis difficult. Various tools are used to diagnose trematode infections: epidemiological criteria, laboratory tests (complete blood count and blood biochemistry, serological methods), instrumental methods (abdominal X-ray and ultrasound), and parasitological techniques, which often have insufficient sensitivity and specificity. Therefore, development of modern and effective non-invasive methods for detection of trematode infections with high sensitivity and specificity, including screening in endemic regions, is relevant.The present review analyzes the results of 90 clinical trials and experimental studies on the diagnosis of trematode infections using the PubMed search engine and the eLibrary database. The review analyzes original articles published from January 1, 2015 to December 31, 2021.Most studies confirm that the absence of a standard diagnostic approach highlights obvious convenience of utilizing a combined approach to reliable diagnosis of trematodiasis. An adequate combination of different diagnostic tests makes it possible to diagnose the disease correctly, devise a correct treatment and follow-up strategy, and organize preventive measures.

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“…Molecular methods are available for F. hepatica detection in fecal samples, such as PCR, loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA). A nested-PCR based on fecal samples may detect the infection at two weeks post infection, while with the LAMP method, the detection time can be as early as one week post infection [ 29 , 30 ]. RPA may be used in areas with limited resources since it does not involve thermal cycler equipment or is dependent on trained personnel [ 31 ].…”

Section: Diagnosismentioning

confidence: 99%

“…Other flukicides only active against adults include: albendazole, clorsulon, closantel, oxyclosantel, nitroxynil and rafoxanide [ 11 , 36 ]. However, resistance has already been reported for some of these [ 30 , 37 , 38 , 39 , 40 ]. To delay the onset of resistance, flukicidal drugs should be used strategically.…”

Section: Treatment and Resistance Towards Flukicidesmentioning

confidence: 99%

Fasciolosis—An Increasing Challenge in the Sheep Industry

Stuen

Ersdal

2022

Animals

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The liver fluke Fasciola hepatica may cause severe infection in several mammalian species, including sheep and humans. Fasciolosis is a parasitic disease occurring worldwide in temperate climates and involves intermediate lymnaeid snails as vectors, in Europe the pond snail Galba truncatula in particular. In the sheep industry, the disease is a serious welfare and health problem. Fasciolosis is usually classified as acute, subacute or chronic according to the number and stage of flukes present in the liver, but with a considerable overlap. Acute disease, associated with a large number of migrating larvae, often results in sudden death due to acute and massive hemorrhage, while chronic fasciolosis is characterized by anemia, hypoalbuminaemia and weight loss. The management of fasciolosis is an increasing challenge in the sheep industry. Early diagnostic tests are limited. Protective immunity against liver flukes in sheep is low or lacking, and vaccines are not yet available. Treatment and control possibilities are challenging, and resistance to flukicide drugs is increasing. In addition, climate change with warmer and more humid weather will have a substantial effect on the establishment of both flukes and snails and will most likely increase the future distribution of F. hepatica.

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Accurate and rapid detection of Fasciola hepatica copro‐DNA in sheep using loop‐mediated isothermal amplification (LAMP) technique

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 13 publications

References 35 publications

Evaluation of Semi-Nested PCR Compared with Indirect-ELISA to Diagnose Human Fasciolosis

Evaluation of Semi-Nested PCR Compared with Indirect-ELISA to Diagnose Human Fasciolosis

Foresight in the diagnosis of trematodiasis: innovations versus routine methods

Fasciolosis—An Increasing Challenge in the Sheep Industry

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