Accurate basophils counting: not an easy goal!

Lesesve, J.‐F.; Benbih, M.; Lecompte, T.

doi:10.1111/j.1365-2257.2005.00667.x

Cited by 24 publications

(13 citation statements)

References 2 publications

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“…The relative low numbers of monocytes in blood cannot be solely accountable for this. Correlation was worst for basophils, in accordance with other blood cell counters Arroyo et al, 2005;Lesesve, Benbih & Lecompte, 2005;Ducrest et al, 2005). This is most likely caused by the very low concentrations of these cells in blood.…”

Section: Discussionsupporting

confidence: 83%

Performance evaluation of the PENTRA 60C⁺ automated hematology analyzer and comparison with the ADVIA 2120

Guerti

Vertessen

Daniels

et al. 2009

Int J Lab Hematology

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The PENTRA 60C(+) hematology analyzer provides a complete blood cell (CBC) count, including a five-part differential (5-DIFF) count and two leukocyte subpopulations, i.e. large immature cells (LIC's) and atypical lymphocytes (ALY's). We evaluated its analytical performance and assessed agreement with the ADVIA 2120, in order to install the analyzer in a small satellite hematology laboratory. First we assessed repeatability, reproducibility and carry-over to evaluate the analytical performance. Then we used Pearson correlation coefficients, Passing and Bablok regression analysis and a graphical approach (n = 209) to evaluate agreement with the ADVIA 2120. Repeatability and reproducibility were excellent for the majority of CBC and 5-DIFF count parameters. Carry-over was negligible. Our data showed very good correlation for most CBC count parameters. Lower correlation coefficients were observed for red cell distribution width, mean corpuscular volume and mean platelet volume. As compared to the ADVIA 2120, the 5-DIFF count performed very well. Agreement was poorer for low-level eosinophils and basophils. Furthermore, the PENTRA 60C(+) was equally able to identify pathological blood samples through the determination of LIC's and ALY's. Therefore, the PENTRA 60C(+) is an eligible blood cell counter to be operational in a satellite laboratory setting.

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Section: Discussionsupporting

confidence: 83%

Performance evaluation of the PENTRA 60C⁺ automated hematology analyzer and comparison with the ADVIA 2120

Guerti

Vertessen

Daniels

et al. 2009

Int J Lab Hematology

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“…6,[16][17][18] In this study, minor outliers in the monocyte data ( Figures 3C and 4C) contributed to the lower correlation coefficients. For basophils, others have shown poor correlation as a result of statistical uncertainty in counting rare events, 19,20 and in this study, all samples contained fewer than 5% basophils.…”

Section: Discussioncontrasting

confidence: 55%

Evaluation of the Beckman Coulter UniCel DxH 800, Beckman Coulter LH 780, and Abbott Diagnostics Cell-Dyn Sapphire Hematology Analyzers on Adult Specimens in a Tertiary Care Hospital

2011

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We evaluated the new Beckman Coulter DxH 800 hematology analyzer (Beckman Coulter, Miami, FL) vs the Abbott Diagnostics Cell-Dyn Sapphire (Abbott Diagnostics, Santa Clara, CA) and Beckman Coulter LH 780 hematology analyzers using 430 adult specimens. The DxH 800 provided a CBC and differential that correlated well with those of the Sapphire and LH 780, with most parameters showing correlation coefficients (r) of more than 0.97. In the instrument vs 400-cell manual differential comparison, all 3 instruments showed similar and acceptable accuracy to the reference method except for nucleated RBC (NRBC) enumeration, in which the DxH 800 and Sapphire outperformed the LH 780. We also compared clinical efficiency by determining whether flagged specimens showed abnormalities on a peripheral blood smear as defined by International Council for Standardization in Haematology criteria. The efficiency, sensitivity, and specificity of the DxH 800 were 77.0%, 87.1%, and 73.0%, respectively, compared with the Sapphire at 75.8%, 93.5%, and 68.8%, respectively, and LH 780 at 66.1%, 93.5%, and 55.3%, respectively.

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“…The result obtained with Pentra MS CRP correlated well with those obtained with our routine analyzers (Sysmex XN‐3000 and Hitachi LABOSPECT), except MON and BAS. The discrepancy in absolute number of BAS between the hematology analyzers examined has been attributed to the rarity of their existence in peripheral blood . The lower, but acceptable correlation for absolute number of MON between Pentra MS CRP and XN‐3000 may have been partially caused by the different mechanism of leukocyte differentials between the two analyzers .…”

Section: Discussionmentioning

confidence: 99%

Basic evaluation of Pentra MS CRP, a new automated hematology analyzer for rapid 5‐part WBC differential and CRP using a small volume of whole blood

Inaba

Nomura

Ishizuka

et al. 2014

Int J Lab Hematology

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Accurate basophils counting: not an easy goal!

Cited by 24 publications

References 2 publications

Performance evaluation of the PENTRA 60C⁺ automated hematology analyzer and comparison with the ADVIA 2120

Performance evaluation of the PENTRA 60C⁺ automated hematology analyzer and comparison with the ADVIA 2120

Evaluation of the Beckman Coulter UniCel DxH 800, Beckman Coulter LH 780, and Abbott Diagnostics Cell-Dyn Sapphire Hematology Analyzers on Adult Specimens in a Tertiary Care Hospital

Basic evaluation of Pentra MS CRP, a new automated hematology analyzer for rapid 5‐part WBC differential and CRP using a small volume of whole blood

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Accurate basophils counting: not an easy goal!

Cited by 24 publications

References 2 publications

Performance evaluation of the PENTRA 60C+ automated hematology analyzer and comparison with the ADVIA 2120

Performance evaluation of the PENTRA 60C+ automated hematology analyzer and comparison with the ADVIA 2120

Evaluation of the Beckman Coulter UniCel DxH 800, Beckman Coulter LH 780, and Abbott Diagnostics Cell-Dyn Sapphire Hematology Analyzers on Adult Specimens in a Tertiary Care Hospital

Basic evaluation of Pentra MS CRP, a new automated hematology analyzer for rapid 5‐part WBC differential and CRP using a small volume of whole blood

Contact Info

Product

Resources

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Performance evaluation of the PENTRA 60C⁺ automated hematology analyzer and comparison with the ADVIA 2120

Performance evaluation of the PENTRA 60C⁺ automated hematology analyzer and comparison with the ADVIA 2120