Accurate nano-injection system for capillary gas chromatography

Zeng, Hulie; Seino, Nobuko; Nakagama, Tatsuro; Kikuchi, Yubun; Nagano, Hazumu; Uchiyama, Katsumi

doi:10.1016/j.chroma.2009.02.051

Cited by 10 publications

(10 citation statements)

References 14 publications

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“…The inkjet printing method belongs to such a drop‐on‐demand technique and is commonly used in printers. The inkjet injection system, derives from the inkjet printing method, can eject droplets from pL to nL with good reproducibility, and has successfully used as an injection method for mass spectrometry (MS) gas chromatography (GC) , enzyme‐linked immunosorbent microarrays , microchannel array electrophoresis , and CE .…”

Section: Introductionmentioning

confidence: 99%

Quantitative on‐line concentration for capillary electrophoresis with inkjet sample introduction technique

Rang

Zeng

Nakajima

et al. 2015

J of Separation Science

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A quantitative sample introduction method based upon inkjet injection was applied to capillary electrophoresis coupled with stacking and sweeping on-line concentration techniques. Methylxanthines were used as model compounds for the proof-of-concept of the method. The volume of injected sample could be easily manipulated by controlling the number of ejected droplets in the injection procedure. Under optimized conditions, a linear relationship between the ejected droplet number and peak area was obtained when the droplet number introduced into the capillary was less than 100. Under optimized quantitative on-line concentration conditions, the limits of detection for theobromine, caffeine, and theophylline were 1.0, 2.0, and 1.0 μM, respectively. The inkjet injection system was evaluated by comparing it with conventional injection methods. The electropherogram of the inkjet injection mode was the same as that for hydrodynamic injection mode, and no sample discrimination was observed compared with the electrokinetic injection mode. The established method was applied to the determination of methylxanthines in bottled green tea. The recoveries of theobromine, caffeine, and theophylline were 94.1, 110.6, and 86.8%, respectively. We conclude that proposed method can be used for quantitative concentration for capillary electrophoresis, thus resulting in an improved accuracy.

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Section: Introductionmentioning

confidence: 99%

Quantitative on‐line concentration for capillary electrophoresis with inkjet sample introduction technique

Rang

Zeng

Nakajima

et al. 2015

J of Separation Science

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“…Yasui et al 20 demonstrated that DNA droplets could be injected with an inkjet injector for microchannel array electrophoresis and were able to achieve the high throughput analysis of biomolecules. Inkjet technology has also been used as an injection tool for gas chromatography (GC), 21 capillary electrophoresis (CE), 22,23 chemiluminescence (CL), 24 and mass spectrometry (MS). 25 However, there are few reports regarding the application of inkjet technology for immunoassays.…”

Section: Introductionmentioning

confidence: 99%

Quantitative-nanoliter immunoassay in capillary immune microreactor adopted inkjet technology

et al. 2014

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“…Actually, the inkjet have great potential for use in automating micro-analytical instrumentation, and have been explored to accurately inject liquid samples for gas chromatography (GC) 42 , capillary electrophoresis (CE) 43 , and in automated enzyme-linked immunosorbent assays (ELISA) 44 . It also has been developed to generate uniform liquid-liquid droplets with a high degree of precision 45 .…”

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Droplet Enhanced Fluorescence for Ultrasensitive Detection Using Inkjet

et al. 2016

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A fluorescence enhanced phenomenon was found within a micrometer-sized liquid droplet, and it was adopted to construct droplet enhanced fluorescence (DEF) for ultrasensitive fluorescence detection. In this paper, an inkjet was utilized to eject perfect spherical droplets to construct a microspherical resonator and to develop a DEF system. It was utilized to implement ultrasensitive fluorescence detection in a liquid specimen with a volume of several microliters. The DEF detection of fluorescent molecules, fluorescein sodium, was used as a model to validate the proposed enhanced fluorescence detection method. A low limit of detection (LOD) for fluorescein sodium of 124 pM was obtained. The sensitive detection of single stranded DNA (ssDNA) was experimentally completed, with a wide range of linearity with a LOD of 312 pM. The proposed mechanism can be used as an ultrasensitive detection technique for analyzing microliters of liquid samples.

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Accurate nano-injection system for capillary gas chromatography

Cited by 10 publications

References 14 publications

Quantitative on‐line concentration for capillary electrophoresis with inkjet sample introduction technique

Quantitative on‐line concentration for capillary electrophoresis with inkjet sample introduction technique

Quantitative-nanoliter immunoassay in capillary immune microreactor adopted inkjet technology

Droplet Enhanced Fluorescence for Ultrasensitive Detection Using Inkjet

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