Acetate Limitation and Nitrite Accumulation during Denitrification

Oh, Jeill; Silverstein, JoAnn

doi:10.1061/(asce)0733-9372(1999)125:3(234)

Cited by 140 publications

(61 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…*1.2) as was observed during lab experiments (Almeida et al 1995;Oh and Silverstein 1999). Furthermore, nitrate reductase is reduced preferentially by electrons derived from acetate oxidation compared to nitrite reductase, which also favors the DNRN reaction over the subsequent denitrification pathway (Almeida et al 1995;Oh and Silverstein 1999), especially when acetate is limited. Nevertheless, the accumulation of nitrite in the interval (up to * 10 mM) did not result in inhibition of the microbial reactivity, as was observed for higher concentrations of nitrite ([20 mM) during lab tests (Almeida et al 1995;Parmentier et al 2014).…”

Section: Impact Of Additional Electron Donors On the In Situ Nitrate mentioning

confidence: 50%

“…Indeed, the C/N ratio after pulse injection of Interval 2 with acetate was 0.4, which is lower than the ratio required to prevent nitrite accumulation and stimulate denitrification (i.e. *1.2) as was observed during lab experiments (Almeida et al 1995;Oh and Silverstein 1999). Furthermore, nitrate reductase is reduced preferentially by electrons derived from acetate oxidation compared to nitrite reductase, which also favors the DNRN reaction over the subsequent denitrification pathway (Almeida et al 1995;Oh and Silverstein 1999), especially when acetate is limited.…”

Section: Impact Of Additional Electron Donors On the In Situ Nitrate mentioning

confidence: 81%

“…1) (Madigan et al 2000). The DNRN pathway usually occurs as an intermediate step in the denitrification process, but can also occur without subsequent denitrification, resulting in the accumulation of nitrite (Almeida et al 1995;Oh and Silverstein 1999). In turn, the produced nitrite can oxidize the clay both biotically and abiotically (Bleyen et al 2015(Bleyen et al , 2016, again possibly resulting in a decrease of the reducing capacity of the clay formation.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Impact of the electron donor on in situ microbial nitrate reduction in Opalinus Clay: results from the Mont Terri rock laboratory (Switzerland)

et al. 2017

View full text Add to dashboard Cite

At the Mont Terri rock laboratory (Switzerland), an in situ experiment is being carried out to examine the fate of nitrate leaching from nitrate-containing bituminized radioactive waste, in a clay host rock for geological disposal. Such a release of nitrate may cause a geochemical perturbation of the clay, possibly affecting some of the favorable characteristics of the host rock. In this in situ experiment, combined transport and reactivity of nitrate is studied inside anoxic and water-saturated chambers in a borehole in the Opalinus Clay. Continuous circulation of the solution from the borehole to the surface equipment allows a regular sampling and online monitoring of its chemical composition. In this paper, in situ microbial nitrate reduction in the Opalinus Clay is discussed, in the presence or absence of additional electron donors relevant for the disposal concept and likely to be released from nitrate-containing bituminized radioactive waste: acetate (simulating bitumen degradation products) and H 2 (originating from radiolysis and corrosion in the repository). The results of these tests indicate that-in case microorganisms would be active in the repository or the surrounding clay-microbial nitrate reduction can occur using electron donors naturally present in the clay (e.g. pyrite, dissolved organic matter). Nevertheless, non-reactive transport of nitrate in the clay is expected to be the main process. In contrast, when easily oxidizable electron donors would be available (e.g. acetate and H 2 ), the microbial activity will be strongly stimulated. Both in the presence of H 2 and acetate, nitrite and nitrogenous gases are predominantly produced, although some ammonium can also be formed when H 2 is present. The reduction of nitrate in the clay could have an impact on the redox conditions in the pore-water and might also lead to a gas-related perturbation of the host rock, depending on the electron donor used during denitrification.

show abstract

Section: Impact Of Additional Electron Donors On the In Situ Nitrate mentioning

confidence: 50%

Section: Impact Of Additional Electron Donors On the In Situ Nitrate mentioning

confidence: 81%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Impact of the electron donor on in situ microbial nitrate reduction in Opalinus Clay: results from the Mont Terri rock laboratory (Switzerland)

et al. 2017

View full text Add to dashboard Cite

show abstract

“…This accumulation nearly immediately disappeared upon addition of methanol as carbon source in the anoxic phase (Martienssen & Schöps 1997). Oh and Silverstein (1999a) on the other hand found that nitrite accumulation during denitrification due to carbon (acetate) limitation (C/N = 1/1) required several weeks to be eliminated after C/N ratio was increased to 2/1.…”

Section: Organic Mattermentioning

confidence: 99%

“…Also the lack or deficiency of organic carbon has been reported to influence the accumulation of nitrite during denitrification (Martienssen et al 1995;Oh & Silverstein 1999a). This accumulation nearly immediately disappeared upon addition of methanol as carbon source in the anoxic phase (Martienssen & Schöps 1997).…”

Section: Organic Mattermentioning

confidence: 99%

Origin, causes and effects of increased nitrite concentrations in aquatic environments

Philips

Laanbroek

Verstraete

2002

Rev Environ Sci Biotechnol

313

198

View full text Add to dashboard Cite

Literature frequently mentions increased nitrite concentrations along with its inhibitory effect towards bacteria and aquatic life. Nitrite accumulation has been studied for decades, and although numerous causal factors have already been commented on in literature, the mechanism of nitrite accumulation is not always clear. From the broad range of parameters and environmental factors reviewed in this paper, it is obvious that the causes and consequences of nitrite accumulation are not yet completely understood. Among others, pH, dissolved oxygen, volatile fatty acids, phosphate and reactor operation have been found to play a role in nitrite accumulation, which results from differential inhibition or disruption of the linkage of the different steps in both nitrification and denitrification. In the case of nitrification, this differential inhibition could lead to the displacement or unlinking of the ammonia oxidisers and nitrite oxidisers. In this paper, the idea is formulated that the nitrifier population forms a role model for the total microbial community. Increased nitrite concentrations would in this aspect not only signal a disruption of nitrifiers, but possibly also of the total configuration of the microbial community.Abbreviations: AMO -Ammonia mono-oxygenase; Anammox -Anaerobic ammonium oxidation; AOBAmmonia-oxidising bacteria; ATP -Adenosine triphosphate; COD -Chemical oxygen demand; DNRA -Dissimilatory nitrate reduction to ammonium; DO -Dissolved oxygen; EU -European union; FA -Free ammonia; F/M -Food to micro-organism ratio; FNA -Free nitrous acid; HAO -Hydroxylamine oxidoreductase; HRTHydraulic residence time; NDBEPR -Enhanced biological phosphate removal with nitrification and denitrification; NO -Nitric oxide; NOB -Nitrite-oxidising bacteria; NOR -Nitrite oxidoreductase; OLAND -Oxygen limited autotrophic nitrification denitrification; RBC -Rotating biological contactor; Sharon -Single reactor high activity ammonia removal over nitrite; SRT -Sludge residence time; TAN -Total ammoniacal nitrogen; TOC -Total organic carbon; VAS -Volatile attached solids

show abstract

Development of a mechanistic model for biological nutrient removal activated sludge systems and application to a full‐scale WWTP

Xie

Liu

et al. 2009

AIChE Journal

View full text Add to dashboard Cite

In wastewater treatment plants (WWTPs) the production of nitrite as an intermediate in the biological nutrient removal (BNR) process has been widely observed, but not been taken into account by most of the conventional activated sludge models yet. This work aims to develop a mechanistic mathematical model to evaluate the BNR process after resolving such a problem. A mathematical model is developed based on the Activated Sludge Model No.3 (ASM3) and the EAWAG Bio-P model with an incorporation of the two-step nitrification-denitrification, the anoxic P uptake, and the associated two-step denitrification by phosphorus accumulating organisms. The database used for simulations originates from a full-scale BNR municipal wastewater treatment plant. The influent wastewater composition is characterized using batch tests. Model predictions are compared with the measured concentrations of chemical oxygen demand (COD), NH Simulation results indicate that the calibrated model is capable of predicting the microbial growth, COD removal, nitrification and denitrification, as well as aerobic and anoxic P removal. Thus, this model can be used to evaluate and simulate full-scale BNR activated sludge WWTPs. V

show abstract

Acetate Limitation and Nitrite Accumulation during Denitrification

Cited by 140 publications

References 10 publications

Impact of the electron donor on in situ microbial nitrate reduction in Opalinus Clay: results from the Mont Terri rock laboratory (Switzerland)

Impact of the electron donor on in situ microbial nitrate reduction in Opalinus Clay: results from the Mont Terri rock laboratory (Switzerland)

Origin, causes and effects of increased nitrite concentrations in aquatic environments

Development of a mechanistic model for biological nutrient removal activated sludge systems and application to a full‐scale WWTP

Contact Info

Product

Resources

About