Peptides are small biomolecule which acts a bioactive or host defense molecules. These peptides have a significant role in medicinal values because of their broad spectrum antimicrobial properties. Marine sources are the largest supply of peptides and natural small molecules, since they represent half of the total global biodiversity. Among the different species of crab, horseshoe crab is widely used sources for the extraction of the peptides. The horseshoe crab is a marine and brackish water arthropod; it plays a crucial role in the reel and whelk fishing industry and the production of important pharmaceutical bioactive components. This review highlights the details of extraction, purification of antimicrobial peptides from horseshoe crab. These peptides trends and prospects for the future, with special emphasis on pharmaceutical industries.