Acetobacter suratthanensis sp. nov., an acetic acid bacterium isolated in Thailand

Pitiwittayakul, Nittaya; Theeragool, Gunjana; Yukphan, Pattaraporn; Chaipitakchonlatarn, Winai; Malimas, Taweesak; Muramatsu, Yuki; Tanasupawat, Somboon; Nakagawa, Yasuyoshi; Yamada, Yoshihiko

doi:10.1007/s13213-016-1200-z

“…Acetic acid bacteria are Gram-stain-negative, obligately aerobic bacteria that produce acetic acid by ethanol oxidation and the genus Acetobacter is a representative acetic acid bacteria [1]. At the time of writing, the genus Acetobacter includes 28 validated published species that have been isolated from various alcoholic fermentation products such as in grapes, wine, water kefir, kombucha, beer, rice wine and cocoa bean and their vinegar products [2][3][4][5][6][7][8][9]. Cells of Acetobacter are motile or non-motile ellipsoidal to short rods usually showing catalase-positive and oxidase-negative activities and containing ubiquinone-9 (Q-9) as the predominant respiratory quinone.…”

mentioning

confidence: 99%

Acetobacter oryzoeni sp. nov., isolated from Korean rice wine vinegar

Baek

¹

,

Kim

²

,

Moon

³

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-negative, obligately aerobic bacterium, designated strain B6T, was isolated from rice wine vinegar in the Republic of Korea. Cells were non-motile and oval short rods showing catalase-positive and oxidase-negative activities. Growth was observed at 15–45 °C (optimum, 30 °C) and pH 3.5–8.0 (optimum, pH 5.5–6.5). Strain B6T contained summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1 ω6c), and C16 : 0 as major fatty acids and ubiquinone-9 was identified as the sole isoprenoid quinone. The G+C content of the genomic DNA calculated from the whole genome was 53.1 mol%. Strain B6T was most closely related to Acetobacter pasteurianus LMG 1262T with very high 16S rRNA gene sequence similarity (100 %) and the strains formed a very close phylogenetic lineage together in phylogenetic trees based on 16S rRNA gene sequences. However, relatedness analyses based on concatenated amino acid sequences of 354 core genes and whole-cell MALDI-TOF profiles showed that strain B6T may form a distinct phyletic lineage from Acetobacter species. In addition, average nucleotide identity and in silico DNA–DNA hybridization values between strain B6T and the type strains of Acetobacter species were less than 93.3 and 51.4 %, respectively. The genomic features of strain B6T were also differentiated from those of closely related Acetobacter type strains. Based on the phenotypic, chemotaxonomic and genomic features, strain B6T clearly represents a novel species of the genus Acetobacter , for which the name Acetobacter oryzoeni sp. nov. is proposed. The type strain is B6T (=KACC 21201T=JCM 33371T).

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“…suratthaniensis TBRC 1719 T and A. papayae LMG 26456 T [38–42] using previously described methods [39, 43–49]. Oxidation of acetate and lactate was analysed by checking the colour change from yellow to blue in a liquid medium [43, 47].…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

“…Ubiquinone-9 was identified as a major quinone in isolates MS16-SU-2 T and MS18-SU-3 with a composition of 91 and 92 %, respectively. The phenotypic characteristics obtained indicated that the isolates clearly belong to the genus Acetobacter [38–42, 53, 54]. The two isolates were distinguished from A.…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

“…The phenotypic characteristics obtained indicated that the isolates clearly belong to the genus Acetobacter [38-42, 53, 54]. The two isolates were distinguished from A. senegalensis TBRC 10 T , A. tropicalis TBRC 19 T , A. orientalis TBRC 962 T , A. suratthaniensis TBRC 1719 T and A. papayae LMG 26456 T [38][39][40][41][42] by absence of growth in media containing glycerol as a sole carbon source, weakly producing acid from methanol and no growth occurring in the presence of 7 % glutamate. The characteristics mentioned above suggested that the two isolates constitute a new species in the genus Acetobacter (Table 2).…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

“…suratthaniensis TBRC 1719 T and A. papayae LMG 26456 T [38–42] by absence of growth in media containing glycerol as a sole carbon source, weakly producing acid from methanol and no growth occurring in the presence of 7 % glutamate. The characteristics mentioned above suggested that the two isolates constitute a new species in the genus Acetobacter ().…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

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Acetobacter garciniae sp. nov., an acetic acid bacterium isolated from fermented mangosteen peel in Thailand

Yukphan

¹

,

Charoenyingcharoen

²

,

Kingcha

³

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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Two isolates, MS16-SU-2T and MS18-SU-3, obtained from fermented mangosteen peel in vinegar were suggested to constitute a new species assignable to the genus Acetobacter based on the results of 16S rRNA gene sequencing. The two isolates showed the highest sequence similarity (98.58%) to Acetobacter tropicalis NBRC 16470T and Acetobacter senegalensis LMG 23690T. However, the calculated similarity values were lower than the threshold for species demarcation. The phylogenetic analysis showed that the branches of the two isolates were separated from other Acetobacter species, and the two isolates constituted a new species in the genus Acetobacter . The genomic DNA of isolate MS16-SU-2T was sequenced. The assembled genome of the isolate was analysed, and the results showed that the highest average nucleotide identity value of 75.9 % was with Acetobacter papayae JCM 25143T and the highest digital DNA–DNA hybridization value of 25.1 % was with Acetobacter fallax LMG 1636T, which were lower than the cutoff values for species delineation. The phylogenetic tree based on the genome sequences showed that the lineage of isolate MS16-SU-2T was most closely related to A. papayae JCM 25143T and Acetobacter suratthaniensis TBRC 1719T, but separated from the branches of these two species. In addition, the two isolates could be distinguished from the type strains of closely related species by their phenotypic characteristics and MALDI-TOF profiles. Therefore, the two isolates, MS16-SU-2T (=TBRC 12339T=LMG 32243T) and MS18-SU-3 (=TBRC 12305), can be assigned to an independent species within the genus Acetobacter , and the name of Acetobacter garciniae sp. nov. is proposed for the two isolates.

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