Acetonic Extract from the Feijoa sellowiana Berg. Fruit Exerts Antioxidant Properties and Modulates Disaccharidases Activities in Human Intestinal Epithelial Cells

Abstract

Feijoa sellowiana fruit has been shown to possess various biological activities, such as anti-bacterial and anti-cancer properties, in a variety of cellular models, but its activity on human intestinal epithelial cells has never been tested. The purpose of this study was to investigate the effects of the acetonic extract of F. sellowiana fruits on the viability, membrane peroxidation, disaccharidases activities and proliferation of in vitro models of human intestinal epithelial cells. To obtain this goal, Caco-2 and HT-29 cells were exposed to the acetonic extract for 24 h. Cell proliferation, viability, lactase and sucrase-isomaltase activity and H2 O2 -induced membrane lipid peroxidation were tested. We found that, compared to control conditions, the acetonic extract significantly increased lactase and sucrase-isomaltase activity in Caco-2, but not HT-29, cells, decreased proliferation, had no effects on viability and restored lipid peroxidation in both cell models. This study suggests that the acetonic extract improves lactase and sucrase-isomaltase activity, inhibits cell proliferation, have no cytotoxic effects and prevent lipid peroxidation of intestinal epithelial cells. These effects may be exploited in case of disaccharidases deficit and also as an adjuvant treatment of diseases related to oxidative stress. Copyright © 2016 John Wiley & Sons, Ltd.

Screening of Cytotoxicity and Anti-Inflammatory Properties of Feijoa Extracts Using Genetically Modified Cell Models Targeting TLR2, TLR4 and NOD2 Pathways, and the Implication for Inflammatory Bowel Disease

“…In the recent decade, the consumption of feijoa has greatly increased and the New Zealand feijoa-processing industries are booming. The health properties of feijoa including antioxidant, antimicrobial and anti-inflammatory activities have been studied [ 3 , 4 , 5 , 6 ], but these studies are far from adequate to elucidate the potential benefit of feijoa against a broad range of diseases.…”

Screening of Feijoa (<i>Acca Sellowiana</i> (<i>O. Berg</i>) <i>Burret</i>) Fruit Effect on Proliferation and Apoptosis using Bone Marrow derived Stem Cells Model

“…Our findings revealed that Feijoa at the dose of ≤ 5 ng/mL significantly increased the proliferation of hBMSCs. The positive in vitro effect of Feijoa on cell viability and proliferation, membrane peroxidation, and disaccharidase in Caco-2 and HT-29 cells was reported before (11). Cellular apoptosis following substance use was previously reported showing an increase in Bax gene and death signaling cascade too (12,13).…”

“…Our findings demonstrated an increasing trend in cell proliferation until day 4 th . The increasing trend in proliferation of hBMSCs during the first 4 days of our study can be related to antioxidant, anti-inflammatory and modulating activities of the Feijoa (4,11,19,20) due to presence of several essential minerals, vitamins, phenolic acids, and polyphenols in the fruit (6). These properties made the fruit to have nutritional value to protect human red blood cells from mercury-induced cellular toxicity (21).…”

“…It also has therapeutic properties for solid and hematological cancers inducing apoptosis in cancer cells by caspase activation, p16, p21 and TRAIL overexpression, increasing the histone and non-histone acetylation and inhibition in HDAC pathway (8,10). The positive in vitro effect of Feijoa on cell viability and proliferation, membrane peroxidation, and disaccharidase in Caco-2 and HT-29 cells was reported before (11). Cellular apoptosis following substance use was previously reported showing an increase in Bax gene and death signaling cascade (12,13).…”

In Vitro Inhibitory Activity of Acca sellowiana Fruit Extract on End Products of Advanced Glycation

“…1 ) [ 6 ]. This species has different pharmacological activities such as antimicrobial [ 7 ], anticancer [ 8 ], anti-inflammatory [ 9 ], and antioxidant [ 10 ] among others. However, to date its ability to inhibit the in vitro glycation reaction has not been reported.…”