Acetonitrile as tops solvent for liquid chromatography and extraction

Рудаков, О. Б.; Rudakova, L. V.; Селеменев, В. Ф.

doi:10.24294/jacs.v1i2.883

Cited by 4 publications

(1 citation statement)

References 27 publications

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“…The final mobile phase components were 70% phosphate buffer (KH 2 PO 4 ) 10 mM, sodium dodecyl sulfate (0.3 mM), and 30% acetonitrile. Pure acetonitrile and its water (buffering) mixtures have unique chromatographic extraction characteristics and significant applications [29]. Fig.…”

Section: Methods Developmentmentioning

confidence: 99%

Bioanalytical Method Validation of Metformin Hydrochloride in Human Plasma by HPLC-UV for Preliminary Population-Based Pharmacokinetic Modeling Study

Adhi Pradana,

Kristin,

Nugroho

et al. 2023

Indones. J. Chem.

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This study aims to validate the method for measuring metformin hydrochloride plasma concentrations using High-Performance Liquid Chromatography (HPLC). This research performed chromatography on a 250 mm 4.6 mm 5 µm purosphere® Star RP-18 column at ambient temperature with a UV detector system at 233 nm. The mobile phase components were 70% phosphate buffer (KH2PO4) (10 mM), sodium dodecyl sulfate (0.3 mM), and 30% acetonitrile. It was pumped at an isocratic flow rate of 1.2 mL/min. Metformin HCl and ranitidine HCl (internal standard) were extracted using acetonitrile. The calibration curve was linear (R2 = 0.9998) in the 0.18–6 µg/mL concentration range. The lower limit of quantification (LLOQ) was 0.18 µg/mL. For intraday accuracy and precision, the percent difference and the coefficient of variation were less than 4 and 7%, and for inter-day were lower than 8 and 6%. The recovery average was 100.96%. The short-term plasma stability test was stable at 24 h at ambient temperature, and the long-term stability test was steady for 30 d at −20 °C. It was also stable after three freeze-thaw cycles. The method meets selectivity, sensitivity, linearity, accuracy, precision, recovery, carryover, and stability requirements and can be applied to population-based pharmacokinetic modeling.

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Section: Methods Developmentmentioning

confidence: 99%