Acetyl-11-keto-β-boswellic acid attenuates titanium particle-induced osteogenic inhibition via activation of the GSK-3β/β-catenin signaling pathway

Xiong, Longbin; Liu, Yu; Zhu, Feng; Lin, Jiayi; Wen, Dongxiang; Wang, Zhen; Bai, Jiaxiang; Ge, Gaoran; Xu, Congxin; Gu, Ye; Xu, Yaozeng; Zhou, Jun

doi:10.7150/thno.35988

Cited by 32 publications

(26 citation statements)

References 62 publications

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“…40 Therefore, potential molecular mechanisms were investigated by examining the expression of key markers, such as β-catenin and p-GSK-3β, which might be involved in the relevant signaling pathways during the osteogenic differentiation of MC3T3-E1 cells. 41,42 As expected, WB analysis demonstrated that the level of β-catenin and p-GSK-3β was increased in Cit-NRIII-treated cells (Figure 5I-K), indicating that the Wnt/β-catenin signaling pathway might play a major role in citrate-stabilized AuNRs-induced osteogenic differentiation in MC3T3-E1 cells.…”

supporting

confidence: 71%

Citrate-Stabilized Gold Nanorods-Directed Osteogenic Differentiation of Multiple Cells

Zhang¹,

Li²,

Liao³

et al. 2021

IJN

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Gold nanorods (AuNRs) show great potential for versatile biomedical applications, such as stem cell therapy and bone tissue engineering. However, as an indispensable shape-directing agent for the growth of AuNRs, cetyltrimethylammonium bromide (CTAB) is not optimal for biological studies because it forms a cytotoxic bilayer on the AuNR surface, which interferes with the interactions with biological cells. Methods: Citrate-stabilized AuNRs with various aspect-ratios (Cit-NRI, Cit-NRII, and Cit-NRIII) were prepared by the combination of end-selective etching and poly(sodium 4-styrenesulfonate)-assisted ligand exchange method. Their effects on osteogenic differentiation of the pre-osteoblastic cell line (MC3T3-E1), rat bone marrow mesenchymal stem cells (rBMSCs), and human periodontal ligament progenitor cells (PDLPs) have been investigated. Potential signaling pathway of citrate-stabilized AuNRs-induced osteogenic effects was also investigated. Results: The experimental results showed that citrate-stabilized AuNRs have superior biocompatibility and undergo aspect-ratio-dependent osteogenic differentiation via expression of osteogenic marker genes, alkaline phosphatase (ALP) activity and formation of mineralized nodule. Furthermore, Wnt/β-catenin signaling pathway might provide a potential explanation for the citrate-stabilized AuNRs-mediated osteogenic differentiation. Conclusion:These findings revealed that citrate-stabilized AuNRs with great biocompatibility could regulate the osteogenic differentiation of multiple cell types through Wnt/βcatenin signaling pathway, which promote innovative AuNRs in the field of tissue engineering and other biomedical applications.

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supporting

confidence: 71%

Citrate-Stabilized Gold Nanorods-Directed Osteogenic Differentiation of Multiple Cells

Zhang¹,

Li²,

Liao³

et al. 2021

IJN

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“…Orally administered AKBA exerts anti-arthritic activity in bovine serum albumin-induced arthritis. Additionally, AKBA inhibits the toll-like receptor-mediated activation of monocytes through the downregulation of LPS-induced nitric oxide, IL-β, and TNF-α production [ 18 , 19 , 20 , 21 ]. Therefore, this study used βBA to develop a therapeutic agent against bone diseases, which has higher absorption efficiency in vivo than AKBA, may be effective as a drug.…”

Section: Discussionmentioning

confidence: 99%

“…There are more than 12 types of BAs of which α-boswellic acid (αBA), β-boswellic acid (βBA), keto β-boswellic acid (KBA), and acetyl-keto β-boswellic acid (AKBA) are the major ones [ 19 ]. Previous studies reported that AKBA inhibits osteoclastogenesis by suppressing the NF-κB-induced TNF-α signaling pathway [ 20 ] and attenuates titanium particle-induced osteogenic inhibition via the GSK-3β/β-catenin signaling pathway [ 21 ]. However, the biological effects of βBA on osteoclast differentiation and bone resorption remain unknown.…”

Section: Introductionmentioning

confidence: 99%

β-Boswellic Acid Inhibits RANKL-Induced Osteoclast Differentiation and Function by Attenuating NF-κB and Btk-PLCγ2 Signaling Pathways

et al. 2021

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Osteoporosis is a systemic metabolic bone disorder that is caused by an imbalance in the functions of osteoclasts and osteoblasts and is characterized by excessive bone resorption by osteoclasts. Targeting osteoclast differentiation and bone resorption is considered a good fundamental solution for overcoming bone diseases. β-boswellic acid (βBA) is a natural compound found in Boswellia serrata, which is an active ingredient with anti-inflammatory, anti-rheumatic, and anti-cancer effects. Here, we explored the anti-resorptive effect of βBA on osteoclastogenesis. βBA significantly inhibited the formation of tartrate-resistant acid phosphatase-positive osteoclasts induced by receptor activator of nuclear factor-B ligand (RANKL) and suppressed bone resorption without any cytotoxicity. Interestingly, βBA significantly inhibited the phosphorylation of IκB, Btk, and PLCγ2 and the degradation of IκB. Additionally, βBA strongly inhibited the mRNA and protein expression of c-Fos and NFATc1 induced by RANKL and subsequently attenuated the expression of osteoclast marker genes, such as OC-STAMP, DC-STAMP, β3-integrin, MMP9, ATP6v0d2, and CtsK. These results suggest that βBA is a potential therapeutic candidate for the treatment of excessive osteoclast-induced bone diseases such as osteoporosis.

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“…The optical density of experimental samples was detected at 450 nm using a microplate-reader (BioTek, Winooski VT, USA) and cell viability was studied. In addition, the cell growth inhibition rate which reflects the extent to which the cell proliferation ability is inhibited compared to the control was converted based on the CCK-8 data (31). In detail, the calculation formula is [growth inhibition rate = (1 − A450 nm value of the drug group/A450 nm value of the control group) × 100%].…”

Section: Cell Viability Assaymentioning

confidence: 99%

Harmine Alleviates Titanium Particle-Induced Inflammatory Bone Destruction by Immunomodulatory Effect on the Macrophage Polarization and Subsequent Osteogenic Differentiation

Wang

et al. 2021

Front. Immunol.

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Peri-prosthetic osteolysis (PPO) and following aseptic loosening are regarded as the prime reasons for implant failure after joint replacement. Increasing evidence indicated that wear-debris-irritated inflammatory response and macrophage polarization state play essential roles in this osteolytic process. Harmine, a β-carboline alkaloid primitively extracted from the Peganum harmala seeds, has been reported to have various pharmacological effects on monoamine oxidase action, insulin intake, vasodilatation and central nervous systems. However, the impact of harmine on debris-induced osteolysis has not been demonstrated, and whether harmine participates in regulating macrophage polarization and subsequent osteogenic differentiation in particle-irritated osteolysis remains unknown. In the present study, we investigated the effect of harmine on titanium (Ti) particle-induced osteolysis in vivo and in vitro. The results suggested harmine notably alleviated Ti particle-induced bone resorption in a murine PPO model. Harmine was also found to suppress the particle-induced inflammatory response and shift the polarization of macrophages from M1 phenotypes to M2 phenotypes in vivo and in vitro, which improved anti-inflammatory and bone-related cytokines levels. In the conditioned medium from Ti particle-stimulated murine macrophage RAW264.7 cells treated with harmine, the osteoblast differentiation ability of mouse pre-osteoblastic MC3T3-E1 cells was greatly increased. And we also provided evidences that the immunomodulatory capacity of harmine might be attributed to the inhibition of the c-Jun N-terminal kinase (JNK) in wear particle-treated macrophages. All the results strongly show that harmine might be a promising therapeutic agent to treat PPO.

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Acetyl-11-keto-β-boswellic acid attenuates titanium particle-induced osteogenic inhibition via activation of the GSK-3β/β-catenin signaling pathway

Cited by 32 publications

References 62 publications

Citrate-Stabilized Gold Nanorods-Directed Osteogenic Differentiation of Multiple Cells

Citrate-Stabilized Gold Nanorods-Directed Osteogenic Differentiation of Multiple Cells

β-Boswellic Acid Inhibits RANKL-Induced Osteoclast Differentiation and Function by Attenuating NF-κB and Btk-PLCγ2 Signaling Pathways

Harmine Alleviates Titanium Particle-Induced Inflammatory Bone Destruction by Immunomodulatory Effect on the Macrophage Polarization and Subsequent Osteogenic Differentiation

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