Acetyl Tributyl Citrate and Dibutyl Sebacate Inhibit the Growth of Cultured Mammalian Cells

Mochida, Kyo; Gomyoda, Manabu; Fujita, Takumi

doi:10.1007/s001289900092

Cited by 9 publications

(5 citation statements)

References 3 publications

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“…However, it was expressed that a little cytotoxic effect of acetyl tributyl citrate was seen on HeLa cells 42 . Mochida et al determined LC50 values of acetyl tributyl citrate in some cells as 39.9 ug/mL on Vero cells and 42.1 ug/mL on Madin‐Darby canine kidney cells 43 . Our results demonstrated that the rate of cytotoxicity of TA was more than TBC.…”

Section: Resultssupporting

confidence: 49%

The effect of alginate scaffolds on bone healing in defects formed with drilling model in rat femur diaphysis

et al. 2023

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Alginate (ALG) is a biocompatible and biodegradable polymer. Mechanical weakness is one of the main problems for the alginate-based scaffolds. Various plasticizer additives or modifications tested to improve the mechanical properties. In the presented study, ALG plasticized with triacetin (TA), and tributyl citrate (TBC) than tested on bone healing. In the presented study, the alginate modified with triacetin or tributyl citrate. In-vitro, and in-vivo efficiency of the scaffolds tested on bone tissue regeneration. Scaffolds fabricated by solvent casting, and physicochemical characterizations performed. Monocytes (THP-1) cultured with scaffolds, and macrophage-released cytokines was determined. In-vivo efficacy of the scaffolds was tested in the rat drill hole model. Alginate and tributyl citrate-modified scaffolds have no cytotoxic effect on osteoblastic cells (MC-3T3). Tributyl citrate modification increased tumor necrosis factor-alpha (TNF-alpha) level but did not increase interleukin -1 beta (IL-1 beta) level. In vivo studies showed that osteoblastic growth was significant in alginate and triacetin-modified scaffolds. However, the best values for osteoclastic activity and osteoid tissue formation seen in the triacetin modification. The results demonstrated that the modified alginate scaffolds were more successful than non-modified alginate scaffolds and can used as long-term bone repairing treatments.

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Section: Resultssupporting

confidence: 49%

The effect of alginate scaffolds on bone healing in defects formed with drilling model in rat femur diaphysis

et al. 2023

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“…Compared to controls, a dose-dependent decrease in growth was noted for all three cell types. ID 50 values were as follows: 44.7 § 2.99 ¹g/ml (KB cells), 39.9 § 2.02 ¹g/ml (Vero cells), and 42.1 § 2.02 ¹g/ml (dog MDCK cells) (Mochida, Gomyoda, and Fujita 1996).…”

Section: Cytotoxicitymentioning

confidence: 99%

Final Report on the Safety Assessment of Acetyl Triethyl Citrate, Acetyl Tributyl Citrate, Acetyl Trihexyl Citrate, and Acetyl Trioctyl Citrate

Johnson¹

2002

Int J Toxicol

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Acetyl Triethyl Citrate, Acetyl Tributyl Citrate, Acetyl Trihexyl Citrate, and Acetyl Trioctyl Citrate all function as plasticizers in cosmetics. Additionally, the Trihexyl and Trioctyl forms are described as skin-conditioning agents-emollients, although there are currently no reported uses of Acetyl Trihexyl Citrate or Acetyl Trioctyl Citrate. Acetyl Triethyl Citrate and Acetyl Tributyl Citrate are used in nail products at concentrations up to 7%. Recognizing that there are no reported uses of Acetyl Trihexyl or Trioctyl Citrate, if they were to be used in the future, their concentration of use is expected to be no higher than that reported for Acetyl Triethyl and Tributyl Citrate. These ingredients were sufficiently similar in structure that safety test data on one were considered applicable to all. Approximately 99% of orally administered Acetyl Tributyl Citrate is excreted-intermediate metabolites include acetyl citrate, monobutyl citrate, acetyl monobutyl citrate, dibutyl citrate, and acetyl dibutyl citrate. In acute, short-term, subchronic, and chronic feeding studies, these ingredients were relatively nontoxic. Differences from controls were either not statistically significant or not related to any organ toxicity. Ocular exposures produced moderate reactions that cleared by 48 hours after instillation. Dermal application was not toxic in rabbits. In a guinea pig maximization test, Acetyl Triethyl Citrate was a sensitizer whereas Acetyl Tributyl Citrate was not. Limited clinical testing of Acetyl Triethyl Citrate and Acetyl Tributyl Citrate was negative for both skin irritation and sensitization. These clinical data were considered more relevant than the guinea pig maximization data, suggesting to the Cosmetic Ingredient Review Expert Panel that none of these ingredients would be a sensitizer. Physiologic effects noted with intravenous delivery of Acetyl Triethyl Citrate or Acetyl Tributyl Citrate include dose-related decreases in blood pressure and intestinal muscular spasms. These ingredients were not genotoxic in bacterial or mammalian test systems. No significant differences in tumor induction (lymphomas) were noted in rats fed Acetyl Tributyl Citrate for 2 year. Acetyl Tributyl Citrate was not a developmental or reproductive toxicant in studies in mice and rats. Based on all the available data, these ingredients were considered safe as used in cosmetics.

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“…Mochida et al . () and Ekwall et al . () published work showing that ATBC can inhibit growth of various mammalian cell lines, including human KB and HeLa cells, monkey Vero cells and dog MDCK cells.…”

Section: Discussionmentioning

confidence: 88%

“…Mechanistic information on ATBC is limited to studies assessing its effects on cell viability and ability to activate nuclear receptors. Mochida et al (1996) and Ekwall et al (1982) published work showing that ATBC can inhibit growth of various mammalian cell lines, including human KB and HeLa cells, monkey Vero cells and dog MDCK cells. Another study aimed at determining the estrogenic potential of phthalate substitutes for use in tissue conditioners in the lining of dentures reported that ATBC does not produce estrogenic responses in MCF-7 cells (Nishijima et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Effects of oral exposure to the phthalate substitute acetyl tributyl citrate on female reproduction in mice

et al. 2016

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Acetyl tributyl citrate (ATBC), is a phthalate substitute used in food and medical plastics, cosmetics, and toys. Although systemically safe up to 1000 mg/kg/day, its ability to cause reproductive toxicity in females at levels below 50 mg/kg/day has not been examined. This study evaluated the effects of lower ATBC exposures on female reproduction using mice. Adult CD-1 females (n=7–8/treatment) were dosed orally with tocopherol-stripped corn oil (vehicle), 5 or 10 mg/kg/day ATBC daily for 15 days, and then bred with a proven breeder male. ATBC exposure did not alter body weights, estrous cyclicity, and gestational and litter parameters. Relative spleen weight was slightly increased in the 5 mg/kg/day group. ATBC at 10 mg/kg/day targeted ovarian follicles and decreased the number of primordial, primary, and secondary follicles present in the ovary. These findings suggest that low levels of ATBC may be detrimental to ovarian function, thus, more information is needed to better understand the impact of ATBC on female reproduction.

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Acetyl Tributyl Citrate and Dibutyl Sebacate Inhibit the Growth of Cultured Mammalian Cells

Cited by 9 publications

References 3 publications

The effect of alginate scaffolds on bone healing in defects formed with drilling model in rat femur diaphysis

The effect of alginate scaffolds on bone healing in defects formed with drilling model in rat femur diaphysis

Final Report on the Safety Assessment of Acetyl Triethyl Citrate, Acetyl Tributyl Citrate, Acetyl Trihexyl Citrate, and Acetyl Trioctyl Citrate

Effects of oral exposure to the phthalate substitute acetyl tributyl citrate on female reproduction in mice

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