Acetylsalicylic acid improved antioxidative status and cold storage of encapsulated nodal segments of neem (Azadirachta indica A. Juss.)

Padilla, Isabel; Barba‐Espín, Gregorio; Hernández, José Antonio; Piqueras, Abel; Burgos, L.

doi:10.1007/s11240-020-01828-z

Cited by 12 publications

(4 citation statements)

References 48 publications

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“…Therefore, subsequent storage experiments were all conducted at 6 °C storage temperature to maintain buds of nodal segments in the dormant status. Similar findings on precocious germination of synseed was observed in neem ( Azadirachta indica ) at 23 °C [ 30 ]. However, no regrowth was observed when the synseeds were incubated at 4 or 8 °C for 1 month [ 30 ].…”

Section: Resultssupporting

confidence: 80%

“…Similar findings on precocious germination of synseed was observed in neem ( Azadirachta indica ) at 23 °C [ 30 ]. However, no regrowth was observed when the synseeds were incubated at 4 or 8 °C for 1 month [ 30 ]. Low temperature (5 °C) was used on cannabis synseeds during 6 months storage with relatively low survival rate (43%) [ 26 ].…”

Section: Resultssupporting

confidence: 80%

“…Sunflower ( Helianthus annuus L.) and Casimairoa edulis shoot tips were encapsulated and stored with 50 µM salicylic acid for 90 days in the dark at 4 °C and viability percent increased from 33 to 59% and 40% to 80%, respectively, compared to controls [ 37 , 38 ]. Similarly, encapsulated neem tree nodal cuttings had an increase in germination rate from 19% to 75% when treated with ASA prior to encapsulation and stored at 12 °C, while a lower (4 °C and 8 °C) temperature treatment significantly hindered germination [ 30 ]. These findings align with our observations which states that synseeds treated with ASA in the dark have improved longevity and higher regrowth rate compared to control ( Figure 3 F).…”

Section: Resultsmentioning

confidence: 99%

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Cannabis Synthetic Seeds: An Alternative Approach for Commercial Scale of Clonal Propagation and Germplasm Conservation

Zarei¹,

Feyissa²,

Davis³

et al. 2022

Plants

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Indoor cannabis (Cannabis sativa) cultivation has been rapidly increasing in many countries after legalization. Besides conventional propagation through cuttings, synthetic seed production provides a competent system for mass propagation, germplasm conservation and international exchange of genetic materials. The present study developed a reliable protocol for cannabis synthetic seed production using encapsulation of nodal segments derived from in vitro or in vivo sources. Synthetic seeds were produced in 3% sodium alginate and 75 mM calcium chloride in Murashige and Skoog (MS) medium and stored under various environmental conditions for up to 150 days. The plantlets regrowth efficiency was monitored on culture media up to 30 days after the storage period. Regrowth rates of 70% and 90% were observed in synthetic seeds from in vitro and in vivo-derived sources, respectively, when stored in 6 °C under 50 μmol s−1 m−2 light for 150 days. Furthermore, addition of acetylsalicylic acid (ASA) to the encapsulation matrix not only postponed precocious germination of synthetic seeds at 22 °C, but also improved the regrowth rate of in vivo-derived synthetic seeds to 100% when they were stored in 6 °C under light. Exposure to light during storage significantly increased shoot length of regrown synseeds when compared to those stored in darkness. This difference in shoot growth disappeared when synseeds were treated with 25 µM ASA. All regenerated plantlets were rooted and acclimatized in sterile rockwool plugs without morphological changes.

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Section: Resultssupporting

confidence: 80%

Section: Resultssupporting

confidence: 80%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Cannabis Synthetic Seeds: An Alternative Approach for Commercial Scale of Clonal Propagation and Germplasm Conservation

Zarei¹,

Feyissa²,

Davis³

et al. 2022

Plants

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show abstract

“…RNA-seq and RT-PCR data showed that parthenolide affected the function of endoplasmic reticulum associated degradation, while proteomic studies indicated that it inhibited the proteolysis of seed storage globulins responsible for the reduced freezing tolerance of the hydrated lettuce seeds. Padilla et al (2021) encapsulated nodal segments of neem (Azadirachta indica A. Juss.)…”

mentioning

confidence: 99%

Cryopreservation and In Vitro banking: a cool subject – Preface from the editors

Ochatt

Lambardi

Panis

et al. 2021

Plant Cell Tiss Organ Cult

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Less Frequently Used Growth Regulators in Plant Tissue Culture

Ochatt

2024

Plant Cell Culture Protocols

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Acetylsalicylic acid improved antioxidative status and cold storage of encapsulated nodal segments of neem (Azadirachta indica A. Juss.)

Cited by 12 publications

References 48 publications

Cannabis Synthetic Seeds: An Alternative Approach for Commercial Scale of Clonal Propagation and Germplasm Conservation

Cannabis Synthetic Seeds: An Alternative Approach for Commercial Scale of Clonal Propagation and Germplasm Conservation

Cryopreservation and In Vitro banking: a cool subject – Preface from the editors

Less Frequently Used Growth Regulators in Plant Tissue Culture

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