Achieving high hybridization density at DNA biosensor surfaces using branched spacer and click chemistry

Kavand, Alireza; Robin, Perrine; Mayoraz, Lucas; Mensi, Mounir; Gerber-Lemaire, Sandrine

doi:10.1039/d3ra04928k

RSC Adv.

2023

DOI: 10.1039/d3ra04928k

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Achieving high hybridization density at DNA biosensor surfaces using branched spacer and click chemistry

Alireza Kavand,

Perrine Robin,

Lucas Mayoraz

et al.

Abstract: Surface functionalization of glass involving peptide-based branched spacer and click chemistry allows for efficient covalent conjugation of single-strand DNA to achieve high hybridization density tailored for biosensing applications.

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2024

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Cited by 2 publications

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Clicking in harmony: exploring the bio-orthogonal overlap in click chemistry

Mehak,

Singh,

Singh

et al. 2024

RSC Adv.

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Clicking in harmony: exploring the bio-orthogonal overlap in click chemistry

Mehak,

Singh,

Singh

et al. 2024

RSC Adv.

View full text Add to dashboard Cite

show abstract

“Grafting-from” and “Grafting-to” Poly(N-isopropyl acrylamide) Functionalization of Glass for DNA Biosensors with Improved Properties

Skigin,

Robin,

Kavand

et al. 2024

Polymers

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Surface-based biosensors have proven to be of particular interest in the monitoring of human pathogens by means of their distinct nucleic acid sequences. Genosensors rely on targeted gene/DNA probe hybridization at the surface of a physical transducer and have been exploited for their high specificity and physicochemical stability. Unfortunately, these sensing materials still face limitations impeding their use in current diagnostic techniques. Most of their shortcomings arise from their suboptimal surface properties, including low hybridization density, inadequate probe orientation, and biofouling. Herein, we describe and compare two functionalization methodologies to immobilize DNA probes on a glass substrate via a thermoresponsive polymer in order to produce genosensors with improved properties. The first methodology relies on the use of a silanization step, followed by PET-RAFT of NIPAM monomers on the coated surface, while the second relies on vinyl sulfone modifications of the substrate, to which the pre-synthetized PNIPAM was grafted to. The functionalized substrates were fully characterized by means of X-ray photoelectron spectroscopy for their surface atomic content, fluorescence assay for their DNA hybridization density, and water contact angle measurements for their thermoresponsive behavior. The antifouling properties were evaluated by fluorescence microscopy. Both immobilization methodologies hold the potential to be applied to the engineering of DNA biosensors with a variety of polymers and other metal oxide surfaces.

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Achieving high hybridization density at DNA biosensor surfaces using branched spacer and click chemistry

Abstract: Surface functionalization of glass involving peptide-based branched spacer and click chemistry allows for efficient covalent conjugation of single-strand DNA to achieve high hybridization density tailored for biosensing applications.

Cited by 2 publications

References 42 publications

Clicking in harmony: exploring the bio-orthogonal overlap in click chemistry

Clicking in harmony: exploring the bio-orthogonal overlap in click chemistry

“Grafting-from” and “Grafting-to” Poly(N-isopropyl acrylamide) Functionalization of Glass for DNA Biosensors with Improved Properties

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