Achromobacter spp. prevalence and adaptation in cystic fibrosis lung infection

Veschetti, Laura; Boaretti, Marzia; Saitta, G; Mantovani, Rebeca Passarelli; Lleò, Maria M.; Sandri, Angela; Malerba, Giovanni

doi:10.1016/j.micres.2022.127140

Cited by 24 publications

(13 citation statements)

References 122 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Conversely, little is known about the pathogenicity of Achromobacter spp. Similar to other CF-related pathogens such as P. aeruginosa , a number of virulence factors have already been described in Achromobacter spp., such as production of secreted factors (e.g., colicin V and proteases), expression of membrane-bound factors (e.g., Vi capsular polysaccharide and O-antigen), secretion systems (e.g., type II and III secretion systems), and biofilm formation, which are important for survival and proliferation in hostile environments [ 5 , 6 , 7 , 8 ]. Moreover, these bacteria show innate and acquired resistance to many classes of antibiotics, especially to aminoglycosides, aztreonam, tetracyclines, penicillins, cephalosporins, and β-lactams [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

In Vivo Inflammation Caused by Achromobacter spp. Cystic Fibrosis Clinical Isolates Exhibiting Different Pathogenic Characteristics

Sandri

Saitta

Veschetti

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

Achromobacter spp. lung infection in cystic fibrosis has been associated with inflammation, increased frequency of exacerbations, and decline of respiratory function. We aimed to evaluate in vivo the inflammatory effects of clinical isolates exhibiting different pathogenic characteristics. Eight clinical isolates were selected based on different pathogenic characteristics previously assessed: virulence in Galleria mellonella larvae, cytotoxicity in human bronchial epithelial cells, and biofilm formation. Acute lung infection was established by intratracheal instillation with 10.5 × 108 bacterial cells in wild-type and CFTR-knockout (KO) mice expressing a luciferase gene under control of interleukin-8 promoter. Lung inflammation was monitored by in vivo bioluminescence imaging up to 48 h after infection, and mortality was recorded up to 96 h. Lung bacterial load was evaluated by CFU count. Virulent isolates caused higher lung inflammation and mice mortality, especially in KO animals. Isolates both virulent and cytotoxic showed higher persistence in mice lungs, while biofilm formation was not associated with lung inflammation, mice mortality, or bacterial persistence. A positive correlation between virulence and lung inflammation was observed. These results indicate that Achromobacter spp. pathogenic characteristics such as virulence and cytotoxicity may be associated with clinically relevant effects and highlight the importance of elucidating their mechanisms.

show abstract

Section: Introductionmentioning

confidence: 99%

In Vivo Inflammation Caused by Achromobacter spp. Cystic Fibrosis Clinical Isolates Exhibiting Different Pathogenic Characteristics

Sandri

Saitta

Veschetti

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…Veschetti ve ark. (20) KF'li 54 hastada kronik ve tekrarlayan enfeksiyonlara sebep olan Achromobacter izolatlarını karşılaştırdıklarında direnç genlerinde önemli bir fark olmadığını bildirmişlerdir. Ayrıca bu mikroorganizmanın antibiyotik direncinden sorumlu mekanizmalarının; beta laktamaz enzim üretimi, akış pompa sistemi veya antibiyotik hedefindeki değişiklikler gibi faktörlerle gerçekleştiğini ifade etmişlerdir.…”

Section: Discussionunclassified

Retrospective analysis of Achromobacter species isolated from cystic fibrosis and non-cystic fibrosis patients

et al. 2022

View full text Add to dashboard Cite

Achromobacter türleri Gram negatif, katalaz, oksidaz ve sitrat pozitif, fermentatif olmayan bakterilerdir. Toplum kökenli ya da hastane kaynaklı enfeksiyonlara sebep olabilmektedir. Hem immünokompetan hem de immün yetmezlikli kişilerde enfeksiyonlara sebep olabilmekle birlikte kistik fibrozis (KF) hastalarını enfekte etmeleri durumunda, KF hastalarının akciğer fonksiyonlarını kötüleştirdiğinden ve daha sık pulmoner alevlenmeye neden olduğundan bu hasta grubunda özellikle önem taşımaktadır. Bu retrospektif çalışmada, Marmara Üniversitesi Eğitim ve Araştırma Hastanesi’nde 2017-2021 yıllarında Achromobacter türleri izole edilen hastaların verileri analiz edilmiştir. Altta yatan hastalıkların varlığına göre Achromobacter türlerinin izolasyon sıklığı, hastalara ait demografik veriler ve antimikrobiyal duyarlılık sonuçları irdelenmiştir. İzolatların tür düzeyinde tanımlaması, matriks ile desteklenmiş lazer desorpsiyon/iyonizasyon uçuş zamanı kütle spektrometresi (MALDI-TOF MS, VITEK MS, BioMérieux, Fransa) ile yapılmıştır. Antimikrobiyal duyarlılık testleri, disk difüzyon metodu ile çalışılmıştır. İstatistiksel analizler için SPSS (Statistical Package for Social Sciences) for Windows 24.0 programı kullanılmıştır. Toplam 148 hastadan 318 Achromobacter izolatı elde edilmiştir. Hastaların %29.7’si kistik fibrozis (KF); %70.3’ü ise KF dışı hastalar olmasına rağmen izolatların %51.6’sı KF hastalarına aittir (P=0.63). En sık gönderilen örnek türü, solunum yollarına ait örnekler olup (%78), KF hastalarında gönderilen örneklerin tümü solunum örneği iken; KF dışı hastalarda bu oran %54.5’tir (P

show abstract

“…[61][62][63][64] Autism has also been associated with both Achromobacter and Pseudomonas genera. 65,66 Both Achromobacter and Pseudomonas are linked to cystic fibrosis, which is another PCD that does not feature neurodegeneration; 67,68 Achromobacter was reported to exacerbate the disease, 69 and P. aeruginosa is a predominant pathogenic species that colonizes the lungs of CF patients. 70 A. xylosoxidans induced proteotoxicity in all our disease models (Figure 4), along with Pseudomonas, which displayed a notable increase in polyQ aggregation (Figure 4A, "Aggregates").…”

Section: Proteotoxic Bacteria Enhance the Aggregation And Toxicity Of...mentioning

confidence: 99%

Identification of proteotoxic and proteoprotective bacteria that non-specifically affect proteins associated with neurodegenerative diseases

Walker,

Bhargava,

Bucher

et al. 2023

Preprint

View full text Add to dashboard Cite

Neurodegenerative protein conformational diseases (PCDs), such as Alzheimer's, Parkinson's, and Huntington's, are a leading cause of death and disability worldwide and have no known cures or effective treatments. Emerging evidence suggests a role for the gut microbiota in the pathogenesis of neurodegenerative PCDs; however, the influence of specific bacteria on the culprit proteins associated with each of these diseases remains elusive, primarily due to the complexity of the microbiota. In the present study, we employed a single-strain screening approach to identify human bacterial isolates that enhance or suppress the aggregation of culprit proteins and the associated toxicity in Caenorhabditis elegans expressing Abeta1-42, alpha-synuclein, and polyglutamine tracts. Here, we reveal the first comprehensive analysis of the human microbiome for its effect on proteins associated with neurodegenerative diseases. Our results suggest that bacteria affect the aggregation of metastable proteins by modulating host proteostasis rather than selectively targeting specific disease-associated proteins. These results reveal bacteria that potentially influence the pathogenesis of PCDs and open new promising prevention and treatment opportunities by altering the abundance of beneficial and detrimental microbes.

show abstract

Achromobacter spp. prevalence and adaptation in cystic fibrosis lung infection

Cited by 24 publications

References 122 publications

In Vivo Inflammation Caused by Achromobacter spp. Cystic Fibrosis Clinical Isolates Exhibiting Different Pathogenic Characteristics

In Vivo Inflammation Caused by Achromobacter spp. Cystic Fibrosis Clinical Isolates Exhibiting Different Pathogenic Characteristics

Retrospective analysis of Achromobacter species isolated from cystic fibrosis and non-cystic fibrosis patients

Identification of proteotoxic and proteoprotective bacteria that non-specifically affect proteins associated with neurodegenerative diseases

Contact Info

Product

Resources

About