Acid-base regulation during exercise and recovery in the blue crab, Callinectes sapidus

Booth, Charles E.; McMahon, Brian R.; Fur, P. L. de; Wilkes, P. R. H.

doi:10.1016/0034-5687(84)90012-4

Cited by 40 publications

(14 citation statements)

References 39 publications

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“…To verify whether prebranchial and postbranchial hemolymph would be comparable we compared hemolymph properties of pre-and postbranchial hemolymph from single individuals. In agreement with previous reports (Booth et al, 1984;Cameron and Batterton, 1978;Rose et al, 1998;Truchot, 1978) there were no significant differences in pH or total CO 2 in prebranchial and postbranchial samples in quiescent animals [For pH: P=0.21 (N=8) at 12°C, P=0.83 (N=9) at 22°C. For total CO 2 : P=0.11 (N=4) at 12°C, P=0.92 (N=3) at 22°C; all values were tested with a paired t-test].…”

Section: Methodssupporting

confidence: 93%

Temperature and acid–base balance in the American lobsterHomarus americanus

Qadri

Camacho

Wang

et al. 2007

Journal of Experimental Biology

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However, thermal change can alter lobster acid-base status over a time course of minutes. Acute increases in temperature trigger a respiratory compensated metabolic acidosis of the hemolymph. Both the strength and frequency of the lobster heartbeat in vitro are modulated by changes in pH within the physiological range measured in vivo. These observations suggest that changes in acid-base status triggered by thermal variations in the environment might modulate lobster cardiac performance in vivo.

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Section: Methodssupporting

confidence: 93%

Temperature and acid–base balance in the American lobsterHomarus americanus

Qadri

Camacho

Wang

et al. 2007

Journal of Experimental Biology

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“…Resting hemolymph lactate (<2mmoll -1 ) was similar to levels measured previously in the blue crab (Booth et al, 1982;Booth and McMahon, 1985;Gannon and Wheatly, 1995;Johnson et al, 2004). A modest 2.2-to 2.5-fold increase in hemolymph lactate in crabs that had walked for 30min was comparable to two-to fourfold increases reported for blue crabs after swimming bouts of 15min (Gannon and Wheatly, 1995) but less than the 14-to 15-fold increase reported after swimming for 25min (Booth et al, 1982) and 30min (Booth and McMahon, 1985;Milligan et al, 1989) and 1h (7-fold increase) (Booth et al, 1984).…”

Section: Discussionmentioning

confidence: 83%

Energy metabolism and metabolic depression during exercise inCallinectes sapidus, the Atlantic blue crab: effects of the bacterial pathogenVibrio campbellii

Thibodeaux

Burnett

2009

Journal of Experimental Biology

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SUMMARYCallinectes sapidus (Rathbun), the Atlantic blue crab, commonly harbors low to moderate amounts of bacteria in hemolymph and other tissues. These bacteria are typically dominated by Vibrio spp., which are known to cause mortality in the blue crab. The dose-dependent lethality of an isolate of Vibrio campbellii was determined in crabs; the mean 48h LD 50 (half-maximal lethal dose) was 6.2ϫ10 5 colony forming unitsg -1 crab. Injection of a sublethal dose of V. campbellii into the hemolymph of the crab resulted in a rapid and large depression (30-42%) of metabolic rate, which persisted for 24h. Because gills are an organ of immune function as well as respiration, we were interested in how bacteria injected into the crab would affect the energetic costs associated with walking. Overall metabolism (aerobic and anaerobic) more than doubled in crabs walking for 30min at 8mmin -1 . The metabolic depression resulting from bacterial injection persisted throughout the exercise period and patterns of phosphagen and adenylate consumption within walking leg muscle were not affected by treatment. The ability of crabs to supply required energy for walking is largely unaffected by exposure to Vibrio; however, Vibrio-injected crabs are less aerobic while doing so. This depressed metabolic condition in response to bacteria, present during moderate activity, could be a passive result of mounting an immune response or may indicate an actively regulated metabolic depression. A compromised metabolism can affect the performance of daily activities, such as feeding and predator avoidance or affect the ability to cope with environmental stressors, such as hypoxia.

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“…In the aquatic crabs, ionic exchanges across the gills are permitted and it is likely that the very large decrease in AH + m in the first hour, which is not associated with lactate removal and is not seen in air, results primarily from branchial excretion of acid or uptake of base (Truchot 1979;Booth et al 1984;Cameron 1985).…”

Section: Metabolic Recoverymentioning

confidence: 98%

Acid‐base disturbances following exercise in a high‐shore crab,Cyclograpsus lavauxi

Waldron

Taylor

Forster

1986

New Zealand Journal of Marine and Freshwater Research

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Cyclograpsus lavauxi, a high-shore crab, suffered a marked acidosis after 10 minutes of moderate exercise in air because of increases in haemolymph lactate concentration and P CO2 . During recovery in sea water, the levels of lactate and P CO2 decreased rapidly and resting pH was restored within 2 h. In air, lactate was also metabolised rapidly, but P CO2 levels declined more slowly than in water and were partly the reason for the continued depression of haemolymph pH at 24 h. There were discrepancies between the concentrations of lactate anions and metabolic protons in the haemolymph during recovery. Protons were removed more rapidly than lactate anions in sea water, which suggests that branchial excretion of acid, and/or uptake of base, was involved in acid-base regulation. The metabolic acid load persisted throughout recovery in air and was, despite possible buffering by mobilisation of CaCO 3 , mainly responsible for the continued depression of haemolymph pH. It is concluded that sea water is necessary for the efficient restoration of haemolymph acid-base status after exercise in this species.

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Acid-base regulation during exercise and recovery in the blue crab, Callinectes sapidus

Cited by 40 publications

References 39 publications

Temperature and acid–base balance in the American lobsterHomarus americanus

Temperature and acid–base balance in the American lobsterHomarus americanus

Energy metabolism and metabolic depression during exercise inCallinectes sapidus, the Atlantic blue crab: effects of the bacterial pathogenVibrio campbellii

Acid‐base disturbances following exercise in a high‐shore crab,Cyclograpsus lavauxi

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