Acid Esterification-Alkaline Transesterification Process for Methyl Ester Production from Crude Rubber Seed Oil

Thaiyasuit, Prachasanti; Pianthong, Kulachate; Worapun, Ittipon

doi:10.5650/jos.61.81

Cited by 33 publications

(25 citation statements)

References 19 publications

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“…Biodiesel is commonly produced from crude oil by alkaline or acid treatment processes, which are known as transesterification [34,35]. Owing to its shorter reaction time and reduced energy consumption, the alkaline-treatment process is the preferred method for transesterification.…”

Section: Discussionmentioning

confidence: 99%

Gene silencing of Sugar-dependent 1 (JcSDP1), encoding a patatin-domain triacylglycerol lipase, enhances seed oil accumulation in Jatropha curcas

Kim

Yang

Mao

et al. 2014

Biotechnol Biofuels

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BackgroundTriacylglycerols (TAGs) are the most abundant form of storage oil in plants. They consist of three fatty acid chains (usually C16 or C18) covalently linked to glycerol. SDP1 is a specific lipase for the first step of TAG catabolism in Arabidopsis seeds. Arabidopsis mutants deficient in SDP1 accumulate high levels of oils, probably due to blockage in TAG degradation. We applied this knowledge from the model plant, Arabidopsis thaliana, to engineer increased seed oil content in the biodiesel plant Jatropha curcas using RNA interference (RNAi) technology.ResultsAs Jatropha is a biodiesel crop, any significant increase in its seed oil content would be an important agronomic trait. Using A. thaliana as a model plant, we found that a deficiency of SDP1 led to higher TAG accumulation and a larger number of oil bodies in seeds compared with wild type (Columbia-0; Col-0). We cloned Jatropha JcSDP1, and verified its function by complementation of the Arabidopsis sdp1-5 mutant. Taking advantage of the observation with Arabidopsis, we used RNAi technology to generate JcSDP1 deficiency in transgenic Jatropha. We found that Jatropha JcSDP1-RNAi plants accumulated 13 to 30% higher total seed storage lipid, along with a 7% compensatory decrease in protein content, compared with control (CK; 35S:GFP) plants. Free fatty acid (FFA) content in seeds was reduced from 27% in control plants to 8.5% in JcSDP1-RNAi plants.ConclusionHere, we showed that SDP1 deficiency enhances seed oil accumulation in Arabidopsis. Based on this result, we generated SDP1-deficient transgenic Jatropha plants using by RNAi technology with a native JcSDP1 promoter to silence endogenous JcSDP1 expression. Seeds of Jatropha JcSDP1-RNAi plants accumulated up to 30% higher total lipid and had reduced FFA content compared with control (CK; 35S:GFP) plants. Our strategy of improving an important agronomic trait of Jatropha can be extended to other oil crops to yield higher seed oil.

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Section: Discussionmentioning

confidence: 99%

Gene silencing of Sugar-dependent 1 (JcSDP1), encoding a patatin-domain triacylglycerol lipase, enhances seed oil accumulation in Jatropha curcas

Kim

Yang

Mao

et al. 2014

Biotechnol Biofuels

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“…Nevertheless, the results are quantitatively similar to those of literature 28 where author reported rapeseed oil methanolysis using KOH catalyst. Thaiyasuit et al 13 also reported 10.5:1 methanol/oil molar ratio for rubber seed oil biodiesel production. The response surface of percentage FAME content as a function of catalyst concentration and temperature at the central levels of methanol to oil molar ratio 8:1 and reaction time of 60 min is shown in Fig.…”

Section: Effect Of Parametersmentioning

confidence: 98%

Optimization and Kinetic Studies on Biodiesel Production from Kusum (<i>Schleichera triguga</i>) Oil Using Response Surface Methodology

2015

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“…Another experiment also used sulfuric acid as a catalyst for the esterification of crude rubber seed oil before trans-esterification. Many researchers choose sulfuric acid because of its high catalytic activity and low cost (Thaiyasuit et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

Multi-Feedstocks Biodiesel Production from Esterification of Calophyllum inophyllum Oil, Castor Oil, Palm Oil and Waste Cooking Oil

Hadiyanto

Aini

Widayat

et al. 2020

Int. J. Renew. Energy Dev.

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Biodiesel can be produced from various vegetable oils and animal fat. Abundant sources of vegetable oil in Indonesia, such as Calophyllum inophyllum, Ricinus communis, palm oil, and waste cooking oil, were used as raw materials. Multi-feedstock biodiesel was used to increase the flexibility operation of biodiesel production. This study was conducted to determine the effect of a combination of vegetable oils on biodiesel characteristics. Degumming and two steps of esterification were applied for high free fatty acid feedstock before trans-esterification in combination with other vegetable oils. Potassium hydroxide was used as a homogenous catalyst and methanol as another raw material. The acid value of C. inophyllum decreased from 54 mg KOH/gr oil to 2.15 mg KOH/gr oil after two steps of esterification. Biodiesel yield from multi-feedstock was 87.926% with a methanol-to-oil molar ratio of 6:1, temperature of 60 ℃, and catalyst of 1%wt.

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Acid Esterification-Alkaline Transesterification Process for Methyl Ester Production from Crude Rubber Seed Oil

Cited by 33 publications

References 19 publications

Gene silencing of Sugar-dependent 1 (JcSDP1), encoding a patatin-domain triacylglycerol lipase, enhances seed oil accumulation in Jatropha curcas

Gene silencing of Sugar-dependent 1 (JcSDP1), encoding a patatin-domain triacylglycerol lipase, enhances seed oil accumulation in Jatropha curcas

Optimization and Kinetic Studies on Biodiesel Production from Kusum (<i>Schleichera triguga</i>) Oil Using Response Surface Methodology

Multi-Feedstocks Biodiesel Production from Esterification of Calophyllum inophyllum Oil, Castor Oil, Palm Oil and Waste Cooking Oil

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