Acinetobacter baumannii Multidrug-Resistant Strain Occurrence in Liver Recipients With Reference to Other High-Risk Groups

Netsvyetayeva, I.; Sikora, Magdalena; Gołaś, Marlena; Swoboda-Kopeć, Ewa; Walthoffen, Szymon Walter de; Dembicka, O.; Frączek, Mariusz; Młynarczyk, A; Pacholczyk, Marek; Chmura, A.; Młynarczyk, G.

doi:10.1016/j.transproceed.2011.07.008

Cited by 7 publications

(5 citation statements)

References 17 publications

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“…Frequent occurrence of hospital infections with A. baumannii is often related to antibiotic misuse or overuse and also due to its ability to persist in hospital environment. In case of A. baumannii infections, its long life span in hospital premises renders its eradication very difficult [13]. The clinical site of infection is an important epidemiological indicator [9].…”

Section: Discussionmentioning

confidence: 99%

The Increased Frequency of Carbapenem Resistant Non Fermenting Gram Negative Pathogens as Causes of Health Care Associated Infections in Adult Cancer Patients

El-Mahallawy¹,

Hamid²,

Hassan³

et al. 2015

JCT

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Background and Aim: Multi drug resistant Non fermenting gram negative bacilli (NFGNB) have emerged as a major cause of health-care associated infections especially in immunocompromised hosts. The aim of the study was to investigate the prevalence of NFGNB as a cause of health-care associated infections (HAI) in cancer patients and determine their resistance pattern. Patients and Methods: During the study period, 158 NFGNB isolates were collected. Microscan Walk Away 9 was used for identification and testing for the metallo-β-lactamases (MBLs) was done by Imipenem-EDTA combined disk synergy test (CDST-IPM). Results: NFGNB represented 29.0% of infections caused by gram negative organisms. Carbapenem resistance, the multi-drug resistant (MDR) phenotype, and MBL production were documented in 70%, 63%, and 59% of NFGNB isolates, respectively. MDR-NFGNB rates were significantly higher among hospitalized patients, medical department and those with longer duration of hospital stay (p = 0.034, 0.026, 0.019; respectively) than non MDR-NFGNB. Conclusion: A high level of carbapenem and multi-drug resistance were detected among the non-fermenter pathogens isolated from hospitalized cases and were more frequently encountered in high risk adult cancer patients requiring longer duration of hospitalization. The MDR-NFGNB are constituting important causes of health-care associated infections in cancer patients.

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Section: Discussionmentioning

confidence: 99%

The Increased Frequency of Carbapenem Resistant Non Fermenting Gram Negative Pathogens as Causes of Health Care Associated Infections in Adult Cancer Patients

El-Mahallawy¹,

Hamid²,

Hassan³

et al. 2015

JCT

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“…The genes encoding carbapenemases class A [e.g., Klebsiella pneumoniae carbapenemase gene, bla KPC ( Li et al, 2014 )], class B [e.g., the metallo-β-lactamases, bla IMP ( Valenzuela et al, 2007 ), bla V IM-1 ( Tsakris et al, 2000 ), bla SIM ( Ellington et al, 2007 ), and bla NDM-1 ( Yong et al, 2009 )], class C [e.g., bla AmpC ( Bou and Martinez-Beltran, 2000 )], and class D [e.g., bla OXA-23 ( Woodford et al, 2006 ), bla OXA-24 ( Woodford et al, 2006 ), bla OXA-51 ( Fu et al, 2010 ), bla OXA-58 ( Netsvyetayeva et al, 2011 ), and bla OXA-235 ( Higgins et al, 2013 )] were investigated by polymerase chain reaction (PCR).…”

Section: Methodsmentioning

confidence: 99%

Characterization of carbapenem-resistant Acinetobacter baumannii isolates in a Chinese teaching hospital

et al. 2015

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Carbapenem-resistant Acinetobacter baumannii (CRAB) presents a serious therapeutic and infection control challenge. In this study, we investigated the epidemiological and molecular differences of CRAB and the threatening factors for contributing to increased CRAB infections at a hospital in western China. A total of 110 clinical isolates of A. baumannii, collected in a recent 2-year period, were tested for carbapenem antibiotic susceptibility, followed by a molecular analysis of carbapenemase genes. Genetic relatedness of the isolates was characterized by multilocus sequence typing. Sixty-seven of the 110 isolates (60.9%) were resistant to carbapenems, 80.60% (54/67) of which carried the blaOXA-23 gene. Most of these CRAB isolates (77.62%) were classified as clone complex 92 (CC92), and sequence type (ST) 92 was the most prevalent STs, followed by ST195, ST136, ST843, and ST75. One CRAB isolate of ST195 harbored plasmid pAB52 from a Chinese patient without travel history. This plasmid contains toxin–antitoxin elements related to adaptation for growth, which might have emerged as a common vehicle indirectly mediating the spread of OXA-23 in CRAB. Thus, CC92 A. baumannii carrying OXA-23 is a major drug-resistant strain spreading in China. Our findings indicate that rational application of antibiotics is indispensable for minimizing widespread of drug resistance.

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“…The genes encoding class A, such as Klebsiella pneumoniae carbapenemase gene ( bla KPC ) (40), class B, such as the metallo-β-lactamase genes [ bla IMP (41), bla VIM-1 (42), bla SIM (43) and bla NDM-1 (44)] and class D, such as CHDLs [ bla OXA-23 (45), bla OXA-24 (45), bla OXA-51 (46) and bla OXA-58 (47)], were also analyzed using PCR. Reaction conditions of PCR were as follows: 94°C for 5 min; and 30 cycles of 94°C for 30 sec, 56°C for 30 sec and 72°C for 30 sec; followed by a final extension at 72°C for 5 min.…”

Section: Methodsmentioning

confidence: 99%

Prevalence of carbapenemases among high-level aminoglycoside-resistant Acinetobacter baumannii isolates in a university hospital in China

Wang

Shen

Yang

et al. 2016

Experimental and Therapeutic Medicine

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The prevalence of aminoglycoside resistant enzymes has previously been reported and extended-spectrum β-lactamase among Acinetobacter baumannii. To track the risk of multidrug-resistant A. baumannii, the present study aimed to determine the prevalence of carbapenemases in high-level aminoglycoside resistant A. baumannii over two years. A total of 118 strains of A. baumannii were consecutively collected in the First Affiliated Hospital of Chengdu Medical College, Chengdu, China. These isolates were investigated on the genetic basis of their resistance to aminoglycosides. The results showed that 75 (63.56%) isolates were high-level resistant to aminoglycosides, including gentamicin and amikacin (minimum inhibitory concentration, ≥256 µg/ml). Aminoglycoside-resistant genes ant(2″)-Ia, aac(6′)-Ib, aph(3′)-Ia, aac(3)-Ia, aac(3)-IIa, armA, rmtA, rmtB, rmtC, rmtD, rmtE, rmtF, rmtG, rmtH and npmA, and carbapenem-resistant genes blaOXA-23, blaOXA-24, blaOXA-51, blaOXA-58, blaSIM, blaIMP, blaNDM-1 and blaKPC, were analyzed using polymerase chain reaction. The positive rate of ant(2″)-Ia, aac(6′)-Ib, aph(3′)-Ia, aac(3)-Ia and aac(3)-IIa was 66.95, 69.49, 42.37, 39.83 and 14.41%, respectively. armA was present in 72.0% (54/75) of A. baumannii isolates with high-level resistance to aminoglycosides. The remaining nine 16S ribosomal RNA methlyase genes (rmtA, rmtB, rmtC, rmtD, rmtE, rmtF, rmtG, rmtH and npmA) and aminoglycoside-modifying enzyme gene aac(6′)-Ib-cr were not detected. Among the 54 armA-positive isolates, the prevalence of the carbapenem resistant blaOXA-23 and blaOXA-51 genes was 79.63 and 100%, respectively. armA, ant(2″)-Ia and aac(6′)-Ib were positive in 43 isolates. The results of multilocus sequence typing revealed 31 sequence types (STs) in all clinical strains. Among these STs, the high-level aminoglycoside-resistant A. baumannii ST92, which mostly harbored blaOXA-23, was the predominant clone (29/75). In conclusion, A. baumannii harboring carbapenemases and aminoglycoside-resistant enzymes are extremely prevalent in western China, emphasizing the need to adopt surveillance programs to solve the therapeutic challenges that this presents.

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Acinetobacter baumannii Multidrug-Resistant Strain Occurrence in Liver Recipients With Reference to Other High-Risk Groups

Cited by 7 publications

References 17 publications

The Increased Frequency of Carbapenem Resistant Non Fermenting Gram Negative Pathogens as Causes of Health Care Associated Infections in Adult Cancer Patients

The Increased Frequency of Carbapenem Resistant Non Fermenting Gram Negative Pathogens as Causes of Health Care Associated Infections in Adult Cancer Patients

Characterization of carbapenem-resistant Acinetobacter baumannii isolates in a Chinese teaching hospital

Prevalence of carbapenemases among high-level aminoglycoside-resistant Acinetobacter baumannii isolates in a university hospital in China

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