Acinetobacter equi sp. nov., isolated from horse faeces

Poppel, Marie T.; Skiebe, Evelyn; Laue, Michael; Bergmann, Holger; Ebersberger, Ingo; Garn, Thomas; Fruth, Angelika; Baumgardt, Sandra; Busse, Hans‐Jürgen; Wilharm, Gottfried

doi:10.1099/ijsem.0.000806

Cited by 26 publications

(15 citation statements)

References 38 publications

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“…Then, oxidative damage of the cluster could trigger disordering or structural change of the C‐terminal end and alter the DNA binding, which in turn can act as a photoreactive agent, in a similar way to that proposed to the W‐triad . This hypothesis may be sustained as FeS‐BCP from Rhodobacter sphaeroides showed [6‐4] photolyase activity ; this kind of proteins had initially been thought to be absent in prokaryotes and in most eukaryotic organisms . Photolyase‐like genes with FeS clusters were confirmed in the two strains of HAAL analyzed in this article, suggesting a possible [6‐4] photolyase activity for them.…”

Section: Resultsmentioning

confidence: 69%

“…(B) Level of amino acid conservation for same alignment, using a Logo image with the distribution Kullback-Leibler, where the triad of tryptophan showed its conserved nature. (73); this kind of proteins had initially been thought to be absent in prokaryotes and in most eukaryotic organisms (75,76). Photolyase-like genes with FeS clusters were confirmed in the two strains of HAAL analyzed in this article, suggesting a possible photolyase activity for them.…”

Section: Conserved Domains and Functional Residues Of Photolyases Andmentioning

confidence: 54%

“…Ver3 presents two CPF sequences: Ver3Phr, a photolyase class I showing 67% identity with the photolyase from Acinetobacter sp. VT 114 (WP_054581756) and Ver3Cry, a putative cryptochrome displaying 95% identity with a cryptochrome from Acinetobacter sp. VT 511 (WP_048881868).…”

Section: Resultsmentioning

confidence: 99%

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Photolyases and Cryptochromes in UV‐resistant Bacteria from High‐altitude Andean Lakes

Portero

Alonso-Reyes

Zannier

et al. 2019

Photochem & Photobiology

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“High‐altitude Andean Lakes” (HAAL) are pristine environments harboring poly‐extremophilic microbes that show combined adaptations to physical and chemical stress such as large daily ambient thermal amplitude, extreme solar radiation levels, intense dryness, alkalinity, high concentrations of arsenic (up to 200 ppm) and dissolved salts. In this work, we compared the UV resistance profiles, pigment content and photoreactivation abilities of three UV‐resistant bacteria isolated from distinct niches from HAALs, that is Acinetobacter sp. Ver3 (water, Lake Verde; 4400 m), Exiguobacterium sp. S17 (stromatolite, Lake Socompa, 3570 m) and Nesterenkonia sp. Act20 (soil, Lake Socompa, 3570 m). UV resistance ability of HAAL's strains indicate a clear adaptation to high radiation exposure encountered in their original habitat, which can be explained by genetic and physiological mechanisms named as the UV‐resistome. Thus, the UV‐resistome depends on the expression of a diverse set of genes devoted to evading or repairing the damage it provoked direct or indirectly. As pigment extraction and photoreactive assays indicate the presence of photoactive molecules, we characterized more in detail proteins with homology to photolyases/cryptochromes members (CPF). Phylogenetic analyses, sequence comparison and 3D modeling with bona fide CPF members were used to prove the presence of functional domains and key residues in the novel proteins.

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Section: Resultsmentioning

confidence: 69%

Section: Conserved Domains and Functional Residues Of Photolyases Andmentioning

confidence: 54%

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Photolyases and Cryptochromes in UV‐resistant Bacteria from High‐altitude Andean Lakes

Portero

Alonso-Reyes

Zannier

et al. 2019

Photochem & Photobiology

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“…Species outside the Acinetobacter calcoaceticus-baumannii complex are collapsed into a single taxon named "Other Acinetobacter". The tree topology follows Poppel et al[62]. (b) Phylogenetic profile of the A. baumannii ATCC 19606 gene cluster encoding the following five proteins: 23S rRNA methyltransferase -Lon protease -Ata -TpgA -Cyclo-ligase.…”

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confidence: 99%

TheAcinetobactertrimeric autotransporter adhesin Ata controls key virulence traits ofAcinetobacter baumannii

et al. 2019

Self Cite

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Acinetobacter baumannii is a Gram-negative pathogen that causes a multitude of nosocomial infections. The Acinetobacter trimeric autotransporter adhesin (Ata) belongs to the superfamily of trimeric autotransporter adhesins which are important virulence factors in many Gram-negative species. Phylogenetic profiling revealed that ata is present in 78% of all sequenced A. baumannii isolates but only in 2% of the closely related species A. calcoaceticus and A. pittii. Employing a markerless ata deletion mutant of A. baumannii ATCC 19606 we show that adhesion to and invasion into human endothelial and epithelial cells depend on Ata. Infection of primary human umbilical cord vein endothelial cells (HUVECs) with A. baumannii led to the secretion of interleukin (IL)-6 and IL-8 in a time- and Ata-dependent manner. Furthermore, infection of HUVECs by WT A. baumannii was associated with higher rates of apoptosis via activation of caspases-3 and caspase-7, but not necrosis, in comparison to ∆ata. Ata deletion mutants were furthermore attenuated in their ability to kill larvae of Galleria mellonella and to survive in larvae when injected at sublethal doses. This indicates that Ata is an important multifunctional virulence factor in A. baumannii that mediates adhesion and invasion, induces apoptosis and contributes to pathogenicity in vivo.

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“…The nucleotide sequence of the PCR amplicon was 99 % homologous to Acinetobacter equi sp. nov. strain 114, submitted to NCBI with the reference sequence: NZ_CP012808.1 [3].…”

Section: Investigationmentioning

confidence: 99%

Challenges in the veterinary microbiology diagnostic laboratory: a novel Acinetobacter species as presumptive cause for feline unilateral conjunctivitis

Vale

Leggett

Smyth³

et al. 2020

Access Microbiology

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The present study highlights challenges in the veterinary microbiology diagnostic laboratory in the identification of bacteria responsible for infections in veterinary settings, particularly when evidence-based data is lacking. A 1.8-year-old neutered male domestic cat (FIV/FeLV negative) was presented to a veterinary practice in April 2016 with a history of left unilateral mild conjunctivitis that was empirically treated with fusidic acid and chloramphenicol. In January 2017, the same animal was presented with chronic left unilateral conjunctivitis and an eye swab was submitted for microbiological culture and susceptibility testing. Significant growth was not detected in two samples tested. Finally, in February 2017 another eye swab produced a slow growing pure culture identified by VITEK 2 as Neisseria cinerea (94 % confidence). Given the morphology and multidrug resistance profile of the isolate a 16S rRNA PCR was performed for definitive identification. The nucleotide sequence of the PCR amplicon was 99 % homologous to Acinetobacter equi sp. nov. strain 114. Veterinary microbiology diagnostic laboratories play an important role worldwide, not only in preserving animal health and welfare but also in controlling the spread of zoonotic pathogens. The lack of evidence-based information on the ocular microbiome of healthy cats and the complexity of bacterial ecosystems renders the interpretation of results difficult. A further problem for both the laboratory and the clinician is the lack of interpretive criteria for antibiotic susceptibility test results for some types of infections in animals (including those caused by Acinetobacter ) and the complete unavailability of criteria for topical antibiotic preparations.

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Acinetobacter equi sp. nov., isolated from horse faeces

Cited by 26 publications

References 38 publications

Photolyases and Cryptochromes in UV‐resistant Bacteria from High‐altitude Andean Lakes

Photolyases and Cryptochromes in UV‐resistant Bacteria from High‐altitude Andean Lakes

TheAcinetobactertrimeric autotransporter adhesin Ata controls key virulence traits ofAcinetobacter baumannii

Challenges in the veterinary microbiology diagnostic laboratory: a novel Acinetobacter species as presumptive cause for feline unilateral conjunctivitis

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