ACL injuries and stem cell therapy

Hirzinger, Corinna; Tauber, Mark; Korntner, Stefanie; Quirchmayr, Michael; Bauer, Hans-Christian; Traweger, Andreas; Tempfer, Herbert

doi:10.1007/s00402-014-2060-2

Cited by 27 publications

(19 citation statements)

References 46 publications

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“…In more recent times, mesenchymal stem cells from different sources have been proposed to augment ACL reconstruction or repair. In this case, flourishing pre-clinical literature suggests that stem cell administration could stimulate tissue maturation, improve histological appearance, and favor bone-to-tendon integration[ 15 , 16 ]. Therefore, the implementation of experimental techniques in the clinical practice seems reasonable.…”

Section: Introductionmentioning

confidence: 99%

Biologic agents for anterior cruciate ligament healing: A systematic review

Matteo¹,

Loibl²,

Andriolo³

et al. 2016

WJO

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AIMTo systematically review the currently available literature concerning the application of biologic agents such as platelet-rich plasma (PRP) and stem cells to promote anterior cruciate ligament (ACL) healing.METHODSA systematic review of the literature was performed on the use of biologic agents (i.e., PRP or stem cells) to favor ACL healing during reconstruction or repair. The following inclusion criteria for relevant articles were used: Clinical reports of any level of evidence, written in English language, on the use of PRP or stem cells during ACL reconstruction/repair. Exclusion criteria were articles written in other languages, reviews, or studies analyzing other applications of PRP/stem cells in knee surgery not related to promoting ACL healing.RESULTSThe database search identified 394 records that were screened. A total of 23 studies were included in the final analysis: In one paper stem cells were applied for ACL healing, in one paper there was a concomitant application of PRP and stem cells, whereas in the remaining 21 papers PRP was used. Based on the ACL injury pattern, two papers investigated biologic agents in ACL partial tears whereas 21 papers in ACL reconstruction. Looking at the quality of the available literature, 17 out of 21 studies dealing with ACL reconstruction were randomized controlled trials. Both studies on ACL repair were case series.CONCLUSIONThere is a paucity of clinical trials investigating the role of stem cells in promoting ACL healing both in case of partial and complete tears. The role of PRP is still controversial and the only advantage emerging from the literature is related to a better graft maturation over time, without documenting beneficial effects in terms of clinical outcome, bone-graft integration and prevention of bony tunnel enlargement.

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Section: Introductionmentioning

confidence: 99%

Biologic agents for anterior cruciate ligament healing: A systematic review

Matteo¹,

Loibl²,

Andriolo³

et al. 2016

WJO

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“…Stem cells, due to their strong self-renewal ability and tissue regeneration capacity, have been studied for the treatment of tissue defect and dysfunction in vitro (5,6). Tendon stem cells have been identified recently and considered an optimal cell source for tendon repair.…”

Section: Introductionmentioning

confidence: 99%

Isolation, culture and biological characteristics of multipotent porcine tendon-derived stem cells

et al. 2018

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Tendon-derived stem cells (TDSCs), a postulated multi-potential stem cell population, play significant role in the postnatal replenishment of tendon injuries. However, the majority of experimental materials were obtained from horse, rat, human and rabbit, but rarely from pig. In this research, 1‑day‑old pig was chosen as experimental sample source to isolate and culture TDSCs in vitro. Specific markers of TDSCs were then characterized by immunofluorescence and reverse transcription polymerase chain reaction (RT‑PCR) assays. The results showed that TDSCs could be expanded for 11 passages in vitro. The expression of specific markers, such as collagen Ⅰ, collagen Ⅲ, α‑smooth muscle actin (α‑SMA), CD105 and CD90 were observed by immunofluorescence and RT‑PCR. TDSCs were induced to differentiate into adipocytes, osteoblasts and chondrocytes, respectively. These results suggest that TDSCs isolated from porcine tendon exhibit the characteristics of multipotent stem cells. TDSCs, therefore, may be potential candidates for cellular transplantation therapy and tissue engineering in tendon injuries.

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“…Furthermore, ACL injury is often associated with an increased risk of early osteoarthritis of the knee [5, 6]. Several methods of ACL reconstruction using autografts or allografts of the hamstrings or patellar tendon have been under investigation in the last decades for the management of ACL tears [1, 7]. However, the long-term outcomes of current reconstruction methods remain unsatisfactory with high failure rates, thus revisions are necessary [8].…”

Section: Introductionmentioning

confidence: 99%

“…The anterior cruciate ligament (ACL) plays an important role in stabilization of the knee joints and is one of the most commonly injured ligaments, particularly in young athletes [1]. In response to injury, the ACL has limited regenerative potential due to its limited blood supply and unique intra-articular environment [2-4].…”

Section: Introductionmentioning

confidence: 99%

Mechanical Loading Improves Tendon-Bone Healing in a Rabbit Anterior Cruciate Ligament Reconstruction Model by Promoting Proliferation and Matrix Formation of Mesenchymal Stem Cells and Tendon Cells

Song

Jiang

Wang

et al. 2017

Cell Physiol Biochem

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Background/Aims: This study investigated the effect of mechanical stress on tendon-bone healing in a rabbit anterior cruciate ligament (ACL) reconstruction model as well as cell proliferation and matrix formation in co-culture of bone-marrow mesenchymal stem cells (BMSCs) and tendon cells (TCs). Methods: The effect of continuous passive motion (CPM) therapy on tendon-bone healing in a rabbit ACL reconstruction model was evaluated by histological analysis, biomechanical testing and gene expressions at the tendon-bone interface. Furthermore, the effect of mechanical stretch on cell proliferation and matrix synthesis in BMSC/TC co-culture was also examined. Results: Postoperative CPM therapy significantly enhanced tendon-bone healing, as evidenced by increased amount of fibrocartilage, elevated ultimate load to failure levels, and up-regulated gene expressions of Collagen I, alkaline phosphatase, osteopontin, Tenascin C and tenomodulin at the tendon-bone junction. In addition, BMSC/TC co-culture treated with mechanical stretch showed a higher rate of cell proliferation and enhanced expressions of Collagen I, Collagen III, alkaline phosphatase, osteopontin, Tenascin C and tenomodulin than that of controls. Conclusion: These results demonstrated that proliferation and differentiation of local precursor cells could be enhanced by mechanical stimulation, which results in enhanced regenerative potential of BMSCs and TCs in tendon-bone healing.

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ACL injuries and stem cell therapy

Cited by 27 publications

References 46 publications

Biologic agents for anterior cruciate ligament healing: A systematic review

Biologic agents for anterior cruciate ligament healing: A systematic review

Isolation, culture and biological characteristics of multipotent porcine tendon-derived stem cells

Mechanical Loading Improves Tendon-Bone Healing in a Rabbit Anterior Cruciate Ligament Reconstruction Model by Promoting Proliferation and Matrix Formation of Mesenchymal Stem Cells and Tendon Cells

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