Acoustic ejection mass spectrometry: Development, applications, and future perspective

Shou, Wilson Z.

doi:10.1002/bmc.5278

Cited by 9 publications

(2 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Echo ® MS system is compatible with standard 384-well or 1536-well injection plates, which are suitable for rapid, high-throughput sample testing. The Echo ® MS system eliminates the need for conventional liquid chromatography consumables such as columns and pre-columns [24][25][26]. Wastewater samples are commonly thought to be difficult to analyze because of their complex matrix.…”

Section: Introductionmentioning

confidence: 99%

Application of Magnetic Materials Combined with Echo® Mass Spectrometry System in Analysis of Illegal Drugs in Sewage

Yang,

Ma,

Cao

et al. 2024

Molecules

View full text Add to dashboard Cite

The aim of this study is to solve the problems of the complicated pretreatment and high analytical cost in the detection technology of trace drugs and their metabolites in municipal wastewater. A high-performance magnetic sorbent was fsynthesized for the enrichment of trace drugs and their metabolites in wastewater to develop a magnetic solid-phase extraction pretreatment combined with the acoustic ejection mass spectrometry (AEMS) analytical method. The magnetic nanospheres were successfully prepared by magnetic nanoparticles modified with divinylbenzene and vinylpyrrolidone. The results showed that the linear dynamic range of 17 drugs was 1–500 ng/mL, the recovery was 44–100%, the matrix effect was more than 51%, the quantification limit was 1–2 ng/mL, and the MS measurement was fast. It can be seen that the developed magnetic solid-phase extraction (MSPE) method is a good solution to the problems of the complicated pretreatment and analytical cost in the analysis of drugs in wastewater. The developed magnetic material and acoustic excitation pretreatment coupled with mass spectrometry analysis method can realize the low-cost, efficient enrichment, and fast analysis of different kinds of drug molecules in urban sewage.

show abstract

Section: Introductionmentioning

confidence: 99%

Application of Magnetic Materials Combined with Echo® Mass Spectrometry System in Analysis of Illegal Drugs in Sewage

Yang,

Ma,

Cao

et al. 2024

Molecules

View full text Add to dashboard Cite

show abstract

“…Recently, ADE-OPI-MS has emerged as a high-throughput method for large-scale screening, − which replaces the part of liquid-phase separation by swiftly introducing the sample into MS using acoustic energy in a contactless manner. This feature helps prevent carryover. , And the analysis time for one sample ranges from 1 to 3 s, and with a minimal sample volume of 2.5 nL.…”

Section: Introductionmentioning

confidence: 99%

High-Throughput Screening for Oligonucleotide Detection by ADE-OPI-MS

Hu,

Zhang,

et al. 2024

Anal. Chem.

View full text Add to dashboard Cite

Oligonucleotides represent a class of shorter DNA or RNA nucleic acid polymers extensively applied in the biomedical field. Despite progress in detecting and analyzing oligonucleotides, high-throughput analysis of the samples remains challenging. In this work, a high-throughput analysis method for oligonucleotide analysis was developed based on acoustic droplet ejection-open port interface-mass spectrometry (ADE-OPI-MS) technology. This approach was applied to determine the enzymatic activity of terminal deoxynucleotide transferase (TdT) for DNA synthesis, with a rate of 3 s/sample, which enhanced single-sample analysis efficiency approximately 60-fold over the previous gel analysis. After testing approximately 10,000 TdT mutants, we obtained three new variants with higher catalytic activities. Finally, by integrating these mutants, the catalytic activity of TdT was improved about 4 times compared to the starting mutant. Our results successfully established a high-throughput screening method for oligonucleotide analysis, which not only provides a foundation to engineer highly efficient TdT for ab initio synthesis of DNA but also paves the way for the potential application of oligonucleotide analysis in biomedical fields.

show abstract

Forty‐Year Evolution of High‐Throughput Mass Spectrometry

Covey

2023

High‐Throughput Mass Spectrometry in Drug Discovery

View full text Add to dashboard Cite

Acoustic ejection mass spectrometry: Development, applications, and future perspective

Cited by 9 publications

References 40 publications

Application of Magnetic Materials Combined with Echo® Mass Spectrometry System in Analysis of Illegal Drugs in Sewage

Application of Magnetic Materials Combined with Echo® Mass Spectrometry System in Analysis of Illegal Drugs in Sewage

High-Throughput Screening for Oligonucleotide Detection by ADE-OPI-MS

Forty‐Year Evolution of High‐Throughput Mass Spectrometry

Contact Info

Product

Resources

About